So can we find a signature of this effect in our inference? Yes. We introduce line tension inference and find that apical and basal line tensions seem to be increased. Especially the basal line tensions can only be explained through additional intracellular physics.

No! Tensions decrease afterwards and, in fact, apical tension and the apico-basal tension difference in buds decrease. We even find anti-correlation of tissue-scale
mean curvature H and cell-scale tension difference. Thus a ratchet-like mechanism has to maintain bud shape.

We next looked at budding intestinal organoids and found that at the time point where budding occurs (48h) apico-basal tension differences are increased, leading to spontaneous curvature. Buds form through apical contraction. Are they stabilized through this mechanism though?

Our method has been extensively tested on in-silico organoids, which we have simulated with our recently introduced bubbly vertex model. Organoids are modeled through cell interfaces subject to surface and line tensions and inference results are tested against ground truth data.

We developed a 3D reconstruction method, where we perform segmentation of organoid image stacks with subsequent geometrical reconstruction in order to use force balance to infer underlying surface and line tensions.

📝 New preprint! 📝
Happy to share a new preprint from @kimboonekamp.bsky.social and me: We developed a 3D force inference method for budding organoids!
biorxiv.org/content/10.1...
From the labs of @ulrichschwarz.bsky.social and
@michaelboutros.bsky.social with help from U. Engel.
Here the details.

Spring brings new beginnings.. Happy to start my new position @istaresearch.bsky.social as a post doc with @ehannezo.bsky.social. Looking forward to learn new things and study new problems in the realm of multicellular structures. Also: Vienna is beautiful.

Time to say goodbye, or rather hello.
A while ago I did a few simulations of plate compression in the 3D vertex model for a publication in Physical Review Research.
This plate, which was compressed a bit too much, nicely resembles the Bsky logo, so let's start the discussion appropriately!