Mr.Att (Attilio Anastasi)
@mr-att.bsky.social
Senior Certified Embryologist 🔬🧪 | #ESHREjc Editor 📝| Referent for Regional Cryobank of gametes and embryos (BUR) ❄️| PGT 🧬| ESHRE5 2022 🧨
Reposted by Mr.Att (Attilio Anastasi)
In the fruit fly embryos, mechanical forces modulate the expression of some genes 😎 we would need to look in humans. Maybe blastoids could help?! #ESHREjc
www.cell.com/fulltext/S15... Tissue Deformation Modulates Twist Expression to Determine Anterior Midgut Differentiation in Drosophila Embryos
www.cell.com/fulltext/S15... Tissue Deformation Modulates Twist Expression to Determine Anterior Midgut Differentiation in Drosophila Embryos
Tissue Deformation Modulates Twist Expression to Determine Anterior Midgut Differentiation in Drosophila Embryos
Mechanical deformations associated with embryonic morphogenetic movements have been
suggested to actively participate in the signaling cascades regulating developmental
gene expression. Here we develo...
www.cell.com
January 30, 2026 at 5:32 PM
In the fruit fly embryos, mechanical forces modulate the expression of some genes 😎 we would need to look in humans. Maybe blastoids could help?! #ESHREjc
www.cell.com/fulltext/S15... Tissue Deformation Modulates Twist Expression to Determine Anterior Midgut Differentiation in Drosophila Embryos
www.cell.com/fulltext/S15... Tissue Deformation Modulates Twist Expression to Determine Anterior Midgut Differentiation in Drosophila Embryos
Reposted by Mr.Att (Attilio Anastasi)
This could also be assessed through the cone formed right after ICSI #ESHREjc
www.sciencedirect.com/science/arti... Characterization of the injection funnel during intracytoplasmic sperm injection reflects cytoplasmic maturity of the oocyte - ScienceDirect
www.sciencedirect.com/science/arti... Characterization of the injection funnel during intracytoplasmic sperm injection reflects cytoplasmic maturity of the oocyte - ScienceDirect
January 30, 2026 at 5:25 PM
This could also be assessed through the cone formed right after ICSI #ESHREjc
www.sciencedirect.com/science/arti... Characterization of the injection funnel during intracytoplasmic sperm injection reflects cytoplasmic maturity of the oocyte - ScienceDirect
www.sciencedirect.com/science/arti... Characterization of the injection funnel during intracytoplasmic sperm injection reflects cytoplasmic maturity of the oocyte - ScienceDirect
Reposted by Mr.Att (Attilio Anastasi)
Q4 waiting for your comments! #ESHREjc
Q4:Given that the cortex is a part of the interface between the oocyte and cumulus cells, how might abnormalities in the follicular environment alter cortical signaling or architecture, and how could these changes be detected through non-invasive follicular fluid or cumulus cell biomarkers?
#ESHREjc
#ESHREjc
a card that says have an egg-stra egg-cellent day with a blue penguin
ALT: a card that says have an egg-stra egg-cellent day with a blue penguin
media.tenor.com
January 30, 2026 at 5:10 PM
Q4 waiting for your comments! #ESHREjc
Reposted by Mr.Att (Attilio Anastasi)
Data so far suggest embryo fragmentation can arise de novo during early cleavages: mitotic chromosome segregation problems keep DNA near the cortex long enough to trigger ectopic actomyosin contractions, essentially a misfired “polar body–like” response. #ESHREjc
pubmed.ncbi.nlm.nih.gov/37427462/
pubmed.ncbi.nlm.nih.gov/37427462/
Cell fragmentation in mouse preimplantation embryos induced by ectopic activation of the polar body extrusion pathway - PubMed
Cell fragmentation is commonly observed in human preimplantation embryos and is associated with poor prognosis during assisted reproductive technology (ART) procedures. However, the mechanisms leading to cell fragmentation remain largely unknown. Here, light sheet microscopy imaging of mouse embryos …
pubmed.ncbi.nlm.nih.gov
January 30, 2026 at 5:07 PM
Data so far suggest embryo fragmentation can arise de novo during early cleavages: mitotic chromosome segregation problems keep DNA near the cortex long enough to trigger ectopic actomyosin contractions, essentially a misfired “polar body–like” response. #ESHREjc
pubmed.ncbi.nlm.nih.gov/37427462/
pubmed.ncbi.nlm.nih.gov/37427462/
Reposted by Mr.Att (Attilio Anastasi)
In mouse, these fragments track poorer progression and lower blastocyst quality/cell number. In humans, fragmentation correlates with poorer prognosis, but the mechanistic link is still a gap. High scale perturbation/imaging is needed. #ESHREjc
January 30, 2026 at 5:09 PM
In mouse, these fragments track poorer progression and lower blastocyst quality/cell number. In humans, fragmentation correlates with poorer prognosis, but the mechanistic link is still a gap. High scale perturbation/imaging is needed. #ESHREjc
Reposted by Mr.Att (Attilio Anastasi)
Altered cumulus cell metabolism or inflammatory signalling could impact cortical actin regulators indirectly, for example via:
⚡️redox imbalance
⚡️altered lipid composition
⚡️disrupted molecule exchange
#ESHREjc
⚡️redox imbalance
⚡️altered lipid composition
⚡️disrupted molecule exchange
#ESHREjc
January 30, 2026 at 5:10 PM
Altered cumulus cell metabolism or inflammatory signalling could impact cortical actin regulators indirectly, for example via:
⚡️redox imbalance
⚡️altered lipid composition
⚡️disrupted molecule exchange
#ESHREjc
⚡️redox imbalance
⚡️altered lipid composition
⚡️disrupted molecule exchange
#ESHREjc
Reposted by Mr.Att (Attilio Anastasi)
Because the cortex mediates oocyte–cumulus communication, metabolic or endocrine stress in the follicle could rewire cortical signalling long before maturation and without obvious morphological changes. #ESHREjc
January 30, 2026 at 5:06 PM
Because the cortex mediates oocyte–cumulus communication, metabolic or endocrine stress in the follicle could rewire cortical signalling long before maturation and without obvious morphological changes. #ESHREjc
Reposted by Mr.Att (Attilio Anastasi)
Reposted by Mr.Att (Attilio Anastasi)
Question 3 on clinical markers of cortical activity is now live. Join the #ESHREjc discussion 👇🏻
Q3. Coticchio et al suggest cortical properties (stiffness, viscoelasticity) could be biomarkers of oocyte developmental competence. Which technologies 🧫🔬🤖could realistically be applied in an IVF lab 👩🏻🔬 to measure cortex mechanics and what are the major barriers to clinical 🧑🏻⚕️ translation? #ESHREjc
a circle with a blue wave in it
ALT: a circle with a blue wave in it
media.tenor.com
January 30, 2026 at 4:53 PM
Question 3 on clinical markers of cortical activity is now live. Join the #ESHREjc discussion 👇🏻
Reposted by Mr.Att (Attilio Anastasi)
There has been important studies suggesting biomechanical properties of oocytes can predict blastocyst development #ESHREjc
www.nature.com/articles/nco... Human oocyte developmental potential is predicted by mechanical properties within hours after fertilization | Nature Communications
www.nature.com/articles/nco... Human oocyte developmental potential is predicted by mechanical properties within hours after fertilization | Nature Communications
Human oocyte developmental potential is predicted by mechanical properties within hours after fertilization - Nature Communications
Reliable assessments of oocyte developmental potential are lacking, making it difficult to select the best quality embryos for transfer after in vitrofertilization. Here, the authors show that a non-i...
www.nature.com
January 30, 2026 at 4:54 PM
There has been important studies suggesting biomechanical properties of oocytes can predict blastocyst development #ESHREjc
www.nature.com/articles/nco... Human oocyte developmental potential is predicted by mechanical properties within hours after fertilization | Nature Communications
www.nature.com/articles/nco... Human oocyte developmental potential is predicted by mechanical properties within hours after fertilization | Nature Communications
Reposted by Mr.Att (Attilio Anastasi)
Cortical stiffness and viscoelasticity likely reflect the underlying actomyosin network organisation, making them interesting markers of oocyte quality that can be more informative than static morphology alone #ESHREjc
January 30, 2026 at 4:54 PM
Cortical stiffness and viscoelasticity likely reflect the underlying actomyosin network organisation, making them interesting markers of oocyte quality that can be more informative than static morphology alone #ESHREjc
Reposted by Mr.Att (Attilio Anastasi)
Techniques like micropipette aspiration or emerging optical microscopy approaches are promising, but translating them to IVF will require meeting the ‘holy grail’ of:
✅ Efficacy
✅ Safety
✅ Cost
✅ Speed of testing
#ESHREjc
✅ Efficacy
✅ Safety
✅ Cost
✅ Speed of testing
#ESHREjc
January 30, 2026 at 4:56 PM
Techniques like micropipette aspiration or emerging optical microscopy approaches are promising, but translating them to IVF will require meeting the ‘holy grail’ of:
✅ Efficacy
✅ Safety
✅ Cost
✅ Speed of testing
#ESHREjc
✅ Efficacy
✅ Safety
✅ Cost
✅ Speed of testing
#ESHREjc
Reposted by Mr.Att (Attilio Anastasi)
Q3. Coticchio et al suggest cortical properties (stiffness, viscoelasticity) could be biomarkers of oocyte developmental competence. Which technologies 🧫🔬🤖could realistically be applied in an IVF lab 👩🏻🔬 to measure cortex mechanics and what are the major barriers to clinical 🧑🏻⚕️ translation? #ESHREjc
a circle with a blue wave in it
ALT: a circle with a blue wave in it
media.tenor.com
January 30, 2026 at 4:49 PM
Q3. Coticchio et al suggest cortical properties (stiffness, viscoelasticity) could be biomarkers of oocyte developmental competence. Which technologies 🧫🔬🤖could realistically be applied in an IVF lab 👩🏻🔬 to measure cortex mechanics and what are the major barriers to clinical 🧑🏻⚕️ translation? #ESHREjc
Reposted by Mr.Att (Attilio Anastasi)
The question is if these cortical defects are already present from the MII stage, or do they emerge only after fertilisation when mitosis initiates on an already compromised cortex? #ESHREjc
January 30, 2026 at 4:35 PM
The question is if these cortical defects are already present from the MII stage, or do they emerge only after fertilisation when mitosis initiates on an already compromised cortex? #ESHREjc
Reposted by Mr.Att (Attilio Anastasi)
Much evidence comes from those models…. Is it time to shift? #ESHREjc
[out of topic, if you are curious ➡️ acd.od.nih.gov/documents/pr...
[out of topic, if you are curious ➡️ acd.od.nih.gov/documents/pr...
January 30, 2026 at 4:34 PM
Much evidence comes from those models…. Is it time to shift? #ESHREjc
[out of topic, if you are curious ➡️ acd.od.nih.gov/documents/pr...
[out of topic, if you are curious ➡️ acd.od.nih.gov/documents/pr...
Reposted by Mr.Att (Attilio Anastasi)
The paper suggests that ectopic chromosomal signalling can induce local cortical weakening or inappropriate contractility, leading to unstable cleavage furrows and fragmentation rather than symmetric cytokinesis. #ESHREjc
January 30, 2026 at 4:31 PM
The paper suggests that ectopic chromosomal signalling can induce local cortical weakening or inappropriate contractility, leading to unstable cleavage furrows and fragmentation rather than symmetric cytokinesis. #ESHREjc
Reposted by Mr.Att (Attilio Anastasi)
Fyn-null oocytes have problems in maintaining cortical integrity....they are subfertile #ESHREjc
Fyn kinase is especially interesting as a signalling integrator. It links chromatin proximity to local cortical remodelling. How direct is the chromatin to cortex signalling axis and are there specific downstream actin regulators involved? #ESHREjc
January 30, 2026 at 4:27 PM
Fyn-null oocytes have problems in maintaining cortical integrity....they are subfertile #ESHREjc
Reposted by Mr.Att (Attilio Anastasi)
The effects of cortical dysregulation in embryo development are discussed in the 2nd question of the #ESHREjc 👇🏻
Q2: The paper proposed that cortical dysregulation can lead to blastomeri fragmentation and reduced blastocyst quality. What are the proposed mechanisms by which ectopic chromosomal signaling or cortical instability leads to abnormal cleavage patterns in early embryos? #ESHREjc
January 30, 2026 at 4:27 PM
The effects of cortical dysregulation in embryo development are discussed in the 2nd question of the #ESHREjc 👇🏻
Reposted by Mr.Att (Attilio Anastasi)