Molecular Microbiology
@molmicrounihoh.bsky.social
https://mikrobiologie.uni-hohenheim.de
Dept. of Molecular Microbiology, University of Hohenheim, Stuttgart
Dept. of Molecular Microbiology, University of Hohenheim, Stuttgart
Pinned
👉 A fascinating example of metabolic plasticity:
Three pathways feed the folate-dependent one carbon pool for growth and virulence of Listeria monocytogenes
doi.org/10.7554/eLif...
@elife.bsky.social #Listeria #folate
Three pathways feed the folate-dependent one carbon pool for growth and virulence of Listeria monocytogenes
doi.org/10.7554/eLif...
@elife.bsky.social #Listeria #folate
doi.org
November 26, 2025 at 7:20 AM
👉 A fascinating example of metabolic plasticity:
Three pathways feed the folate-dependent one carbon pool for growth and virulence of Listeria monocytogenes
doi.org/10.7554/eLif...
@elife.bsky.social #Listeria #folate
Three pathways feed the folate-dependent one carbon pool for growth and virulence of Listeria monocytogenes
doi.org/10.7554/eLif...
@elife.bsky.social #Listeria #folate
what you select is what you get
November 25, 2025 at 7:31 PM
what you select is what you get
🫴 A ubiquitin-like protein controls assembly of a bacterial type VIIb secretion system:
| Science Advances www.science.org/doi/10.1126/...
#subtiwiki
| Science Advances www.science.org/doi/10.1126/...
#subtiwiki
A ubiquitin-like protein controls assembly of a bacterial type VIIb secretion system
Cryo-EM structure reveals how a ubiquitin-like protein orchestrates assembly of a bacterial weapon system.
www.science.org
November 24, 2025 at 12:31 PM
🫴 A ubiquitin-like protein controls assembly of a bacterial type VIIb secretion system:
| Science Advances www.science.org/doi/10.1126/...
#subtiwiki
| Science Advances www.science.org/doi/10.1126/...
#subtiwiki
😳 speechless. today in the lecture hall...
November 20, 2025 at 5:06 PM
😳 speechless. today in the lecture hall...
Repair of damaged intermediates in Bacillus subtilis: Three N-acetylcysteine deacetylases can catalyze the final step in S-(2-succino)cysteine breakdown!
In 👉 Journal of Biological Chemistry
www.jbc.org/article/S002...
#subtiwiki
In 👉 Journal of Biological Chemistry
www.jbc.org/article/S002...
#subtiwiki
Bacillus subtilis encodes three N-acetylcysteine deacetylase enzymes that can catalyze the final step in S-(2-succino)cysteine breakdown
Succination occurs when the TCA cycle intermediate fumarate reacts with cellular thiols,
such as cysteine, yielding the damaged metabolite S-(2-succino)cysteine (2SC). Increased
fumarate levels result...
www.jbc.org
November 20, 2025 at 7:52 AM
Repair of damaged intermediates in Bacillus subtilis: Three N-acetylcysteine deacetylases can catalyze the final step in S-(2-succino)cysteine breakdown!
In 👉 Journal of Biological Chemistry
www.jbc.org/article/S002...
#subtiwiki
In 👉 Journal of Biological Chemistry
www.jbc.org/article/S002...
#subtiwiki
👉 Growth of Corynebacterium glutamicum on 5-oxo-L-proline (pyroglutamate) as carbon and nitrogen source requires the PxpR-controlled pxpTABC genes:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Growth of Corynebacterium glutamicum on 5-oxo-L-proline (pyroglutamate) as carbon and nitrogen source requires the PxpR-controlled pxpTABC genes
5-Oxo-L-proline (5-OP) is inevitably formed in all cells by spontaneous cyclization of L-glutamate, L-glutamine, or γ-glutamyl phosphate. Its use as a substrate by bacteria has rarely been described. ...
www.biorxiv.org
November 19, 2025 at 2:48 PM
👉 Growth of Corynebacterium glutamicum on 5-oxo-L-proline (pyroglutamate) as carbon and nitrogen source requires the PxpR-controlled pxpTABC genes:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
don't forget!
November 19, 2025 at 9:39 AM
don't forget!
Great: Cross-kingdom RNA trafficking from bacteria (including Bacillus subtilis) to fungi enables plant protection against fungal pathogens!
👇
Molecular Plant www.cell.com/molecular-pl...
#subtiwiki #subtilis
👇
Molecular Plant www.cell.com/molecular-pl...
#subtiwiki #subtilis
Cross-kingdom RNA trafficking from bacteria to fungi enables plant protection against fungal pathogens
In this study we engineered gram-positive and gram-negative beneficial bacteria to
produce double-stranded RNAs targeting essential genes in important fungal pathogens.
We demonstrate that RNAs can be...
www.cell.com
November 13, 2025 at 7:21 PM
Great: Cross-kingdom RNA trafficking from bacteria (including Bacillus subtilis) to fungi enables plant protection against fungal pathogens!
👇
Molecular Plant www.cell.com/molecular-pl...
#subtiwiki #subtilis
👇
Molecular Plant www.cell.com/molecular-pl...
#subtiwiki #subtilis
Structure and encapsulation of carbonic anhydrase within the α-carboxysome from Halothiobacillus neapolitanus:
www.pnas.org/doi/10.1073/...
@selimlab.bsky.social Probably interesting for the SPP initiative.
@pnas.org
www.pnas.org/doi/10.1073/...
@selimlab.bsky.social Probably interesting for the SPP initiative.
@pnas.org
Structure and encapsulation of carbonic anhydrase within the α-carboxysome | PNAS
Carboxysomes in cyanobacteria and certain proteobacteria enable efficient CO2 fixation
by encapsulating ribulose-1,5-bisphosphate carboxylase/oxyge...
www.pnas.org
November 13, 2025 at 12:12 PM
Structure and encapsulation of carbonic anhydrase within the α-carboxysome from Halothiobacillus neapolitanus:
www.pnas.org/doi/10.1073/...
@selimlab.bsky.social Probably interesting for the SPP initiative.
@pnas.org
www.pnas.org/doi/10.1073/...
@selimlab.bsky.social Probably interesting for the SPP initiative.
@pnas.org
Fascinating: Glycogen phase-separation drives macromolecular rearrangement and asymmetric division in E. coli:
👇
| The EMBO Journal www.embopress.org/doi/full/10....
@embojournal.org #coli
👇
| The EMBO Journal www.embopress.org/doi/full/10....
@embojournal.org #coli
Glycogen phase-separation drives macromolecular rearrangement and asymmetric division in E. coli | The EMBO Journal
imageimageSubcellular organization of bacterial cells transitioning from exponential to stationary
phase has not been well investigated. This study shows that transition-phase E. coli cells exhibit as...
www.embopress.org
November 13, 2025 at 12:09 PM
Fascinating: Glycogen phase-separation drives macromolecular rearrangement and asymmetric division in E. coli:
👇
| The EMBO Journal www.embopress.org/doi/full/10....
@embojournal.org #coli
👇
| The EMBO Journal www.embopress.org/doi/full/10....
@embojournal.org #coli
wobbly Tuesday
November 11, 2025 at 8:43 AM
wobbly Tuesday
Reposted by Molecular Microbiology
S. aureus has an intrinsic rifampicin resistance mechanism! See the recent study by M. Otto's lab with important clinical implications:
Export by a quorum sensing–controlled drug exporter underlies rifampicin’s strong activity against staphylococcal biofilms | www.science.org/doi/10.1126/...
Export by a quorum sensing–controlled drug exporter underlies rifampicin’s strong activity against staphylococcal biofilms | www.science.org/doi/10.1126/...
Export by a quorum sensing–controlled drug exporter underlies rifampicin’s strong activity against staphylococcal biofilms
Rifampicin is more effective against biofilm infections because it targets biofilm-forming mutants that are highly resistant to other antibiotics.
www.science.org
November 10, 2025 at 4:03 PM
S. aureus has an intrinsic rifampicin resistance mechanism! See the recent study by M. Otto's lab with important clinical implications:
Export by a quorum sensing–controlled drug exporter underlies rifampicin’s strong activity against staphylococcal biofilms | www.science.org/doi/10.1126/...
Export by a quorum sensing–controlled drug exporter underlies rifampicin’s strong activity against staphylococcal biofilms | www.science.org/doi/10.1126/...
🫵 Our daily bread: Experimental evolution of gene essentiality in bacteria!
| mBio journals.asm.org/doi/10.1128/...
@mbio.bsky.social
| mBio journals.asm.org/doi/10.1128/...
@mbio.bsky.social
Experimental evolution of gene essentiality in bacteria | mBio
Essential genes are traditionally considered indispensable for bacterial survival,
but how they interact with other cellular processes is not well understood. Here,
we deleted essential genes from div...
journals.asm.org
November 10, 2025 at 8:13 PM
🫵 Our daily bread: Experimental evolution of gene essentiality in bacteria!
| mBio journals.asm.org/doi/10.1128/...
@mbio.bsky.social
| mBio journals.asm.org/doi/10.1128/...
@mbio.bsky.social
Conflicts between the DNA replication and repair machineries promote cell death in Gram-positive bacteria:
www.microbiologyresearch.org/content/jour...
@microbiologysociety.org
www.microbiologyresearch.org/content/jour...
@microbiologysociety.org
Conflicts between the DNA replication and repair machineries promote cell death in Gram-positive bacteria
Cellular proliferation relies on the successful coordination and completion of genome replication and segregation. To help achieve this, many bacteria utilize regulatory pathways that ensure DNA repli...
www.microbiologyresearch.org
November 10, 2025 at 8:32 AM
Conflicts between the DNA replication and repair machineries promote cell death in Gram-positive bacteria:
www.microbiologyresearch.org/content/jour...
@microbiologysociety.org
www.microbiologyresearch.org/content/jour...
@microbiologysociety.org
🫳 The stringent response does not influence ribosome pausing in Bacillus subtilis:
academic.oup.com/nar/article/...
@narjournal.bsky.social #subtiwiki
academic.oup.com/nar/article/...
@narjournal.bsky.social #subtiwiki
The stringent response does not influence ribosome pausing in Bacillus subtilis
Abstract. The stringent response represses translation and is activated when cells enter the stationary phase and intracellular amino acid levels drop. Bac
academic.oup.com
November 1, 2025 at 4:48 PM
🫳 The stringent response does not influence ribosome pausing in Bacillus subtilis:
academic.oup.com/nar/article/...
@narjournal.bsky.social #subtiwiki
academic.oup.com/nar/article/...
@narjournal.bsky.social #subtiwiki
🫴 FrlP, an ABC type I importer component of Bacillus subtilis: regulation and impact in bacterial fitness:
| Journal of Bacteriology journals.asm.org/doi/10.1128/...
@jbacteria.bsky.social
| Journal of Bacteriology journals.asm.org/doi/10.1128/...
@jbacteria.bsky.social
FrlP, an ABC type I importer component of Bacillus subtilis: regulation and impact in bacterial fitness | Journal of Bacteriology
Bacillus subtilis is widely applied in the industry as a microbial cell factory, as a biofertilizer
for sustainable agriculture, in the animal feed industry and as human probiotic. In
its natural envi...
journals.asm.org
October 31, 2025 at 5:09 PM
🫴 FrlP, an ABC type I importer component of Bacillus subtilis: regulation and impact in bacterial fitness:
| Journal of Bacteriology journals.asm.org/doi/10.1128/...
@jbacteria.bsky.social
| Journal of Bacteriology journals.asm.org/doi/10.1128/...
@jbacteria.bsky.social
Lab outing to Stuttgart Airport (STR) with interesting insights behind the scenes! 🛩️ 🛫 ✈️
October 31, 2025 at 4:26 PM
Lab outing to Stuttgart Airport (STR) with interesting insights behind the scenes! 🛩️ 🛫 ✈️
Reposted by Molecular Microbiology
Cool new paper on the structure and possible function of the type VII ABC transporter YtrEF in cell wall remodeling in Bacillus subtilis!
#SubtiWiki
www.sciencedirect.com/science/arti...
#SubtiWiki
www.sciencedirect.com/science/arti...
Structural analysis of a Gram-positive type VII ABC transporter induced by cell wall-targeting antibiotics
Bacteria utilize a variety of mechanisms to remodel the cell wall in response to environmental and antimicrobial stress. In the model organism Bacillu…
www.sciencedirect.com
October 29, 2025 at 3:28 PM
Cool new paper on the structure and possible function of the type VII ABC transporter YtrEF in cell wall remodeling in Bacillus subtilis!
#SubtiWiki
www.sciencedirect.com/science/arti...
#SubtiWiki
www.sciencedirect.com/science/arti...
🫴 Structure of Bacillus subtilis Ku-mediated DNA synaptic complex:
👇
academic.oup.com/nar/article/...
👇
academic.oup.com/nar/article/...
Structure of Bacillus subtilis Ku-mediated DNA synaptic complex
Abstract. DNA double-strand breaks (DSBs) pose a severe threat to genomic integrity, and cells rely on two major pathways for repair: homologous recombinat
academic.oup.com
October 22, 2025 at 7:01 AM
🫴 Structure of Bacillus subtilis Ku-mediated DNA synaptic complex:
👇
academic.oup.com/nar/article/...
👇
academic.oup.com/nar/article/...
Weird: A broadly conserved fungal chorismate mutase targets the plant shikimate pathway to regulate salicylic acid production and other secondary metabolites:
| mBio journals.asm.org/doi/10.1128/...
| mBio journals.asm.org/doi/10.1128/...
A broadly conserved fungal chorismate mutase targets the plant shikimate pathway to regulate salicylic acid production and other secondary metabolites | mBio
Microbial effectors are small secreted proteins that help pathogens establish disease
within the host environment. In biotrophic fungi, secreted chorismate mutases (CMs)
like Cmu1 suppress the product...
journals.asm.org
October 21, 2025 at 9:40 AM
Weird: A broadly conserved fungal chorismate mutase targets the plant shikimate pathway to regulate salicylic acid production and other secondary metabolites:
| mBio journals.asm.org/doi/10.1128/...
| mBio journals.asm.org/doi/10.1128/...
October 20, 2025 at 11:18 AM
October 20, 2025 at 11:18 AM
🫴 Short tandem gene duplications as potential agents of genetic memory:
| mBio journals.asm.org/doi/10.1128/...
@mbio.bsky.social #Pseudomonas
| mBio journals.asm.org/doi/10.1128/...
@mbio.bsky.social #Pseudomonas
Short tandem gene duplications as potential agents of genetic memory | mBio
Unstable mutations, such as tandem gene duplications, contribute significantly to
the evolution of antibiotic resistance and other traits, but their importance is often
underestimated. In this study, ...
journals.asm.org
October 17, 2025 at 4:48 AM
🫴 Short tandem gene duplications as potential agents of genetic memory:
| mBio journals.asm.org/doi/10.1128/...
@mbio.bsky.social #Pseudomonas
| mBio journals.asm.org/doi/10.1128/...
@mbio.bsky.social #Pseudomonas
Simply amazing: Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail:
| Science Advances www.science.org/doi/10.1126/...
@spp2330.bsky.social
| Science Advances www.science.org/doi/10.1126/...
@spp2330.bsky.social
Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail
This structure of an archaeal tailed virus (arTV) provides detailed insights into arTV assembly and infection mechanisms.
www.science.org
October 16, 2025 at 7:35 PM
Simply amazing: Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail:
| Science Advances www.science.org/doi/10.1126/...
@spp2330.bsky.social
| Science Advances www.science.org/doi/10.1126/...
@spp2330.bsky.social
🫴 Molecular basis for noncanonical transcription initiation from Np4A alarmones:
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Molecular basis for noncanonical transcription initiation from Np4A alarmones - Nature Chemical Biology
Duan and Kaushik et al. reveal the structural basis of how Escherichia coli and Thermus thermophilus RNA polymerases initiate transcription from Np4A alarmones producing Np4-capped transcripts. The ca...
www.nature.com
October 15, 2025 at 2:32 PM
🫴 Molecular basis for noncanonical transcription initiation from Np4A alarmones:
www.nature.com/articles/s41...
www.nature.com/articles/s41...