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molecularmicro.bsky.social
Molecular Microbiology Editors
@molecularmicro.bsky.social
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molecularmicro.bsky.social
Trussler et al. use comparative RNA-seq to explore how an sRNA derived from a transposon affects >200 genes and acts as global regulator of Salmonella invasion and flagellar genes.
onlinelibrary.wiley.com/doi/10.1111/...
#MicroSky #sRNA
A Small RNA Derived From the 5′ End of the IS200 tnpA Transcript Regulates Multiple Virulence Regulons in Salmonella typhimurium
We show deletion of an IS200-encoded sRNA (5′tnpA) in Salmonella impacts the expression of SPI-1, flagellar, cysteine, and phsABC regulons. Evidence is presented that, with regard to the SPI-1 regulo...
onlinelibrary.wiley.com
August 25, 2025 at 11:41 AM
molecularmicro.bsky.social
Dong, Zou, and Cronan report the fatty acid preferences of the multiple FakB fatty acid binding proteins that function with FakB to convert exogenous fatty acids to acyl phosphates in Enterococcus faecalis.
onlinelibrary.wiley.com/doi/10.1111/...
#MicroSky
Defining the Enterococcus faecalis Fatty Acid Kinase System of Exogeneous Fatty Acid Utilization
The enteric bacterium Enterococcus faecalis incorporates fatty acids from the medium and elongates any short chains to lengths suitable for membrane lipids. We have delineated the pathway.
onlinelibrary.wiley.com
August 25, 2025 at 11:36 AM
molecularmicro.bsky.social
Fig4 is a lipid phosphatase conserved in all eukaryotes and is associated with human neurological diseases. Using S. cerevisiae, Reeves et al. explore how Fig4 disease-related mutants alter TORC1 signaling.
onlinelibrary.wiley.com/doi/10.1111/...
#MicroSky
Homeostatic Influence of Fig4 Outside of the Fab1‐Vac14‐Fig4 Complex in Saccharomyces cerevisiae
Disease-related Fig4 mutants displaying impaired association with the Fab1-Vac14-Fig4 complex confer rapamycin tolerance in Saccharomyces cerevisiae independent of Vac14 and Fig4 catalytic function. ...
onlinelibrary.wiley.com
August 25, 2025 at 11:33 AM
molecularmicro.bsky.social
RibR regulatory proteins bind to FMN-sensing riboswitches, override their genetic decisions, and stimulate riboflavin gene expression. Hübenthal and Mack show that RibR-like regulator proteins may be more widespread than anticipated.
onlinelibrary.wiley.com/doi/10.1111/...
#MicroSky
The Monofunctional FMN Riboswitch‐Binding Regulator RibR From Bacillus amyloliquefaciens Promotes Riboflavin Biosynthesis
Riboswitches are RNA molecules that control gene expression and have been hypothesized to function independently of proteins. The monofunctional regulator RibR from the bacterium Bacillus amyloliquef...
onlinelibrary.wiley.com
August 25, 2025 at 11:30 AM
molecularmicro.bsky.social
Zhang et al. show that adaptation of the plant pathogenic fungus Botrytis cinerea to mild heat stress requires both autophagy and the mitochondrial Lon1 protease.
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
Autophagy and the Mitochondrial Lon1 Protease Are Necessary for Botrytis cinerea Heat Adaptation
The destabilization of mitochondria due to the disruption of the Lon1 protease induces cellular damage, including reactive oxygen species (ROS) accumulation, impaired germination, and growth arrest. ....
onlinelibrary.wiley.com
July 21, 2025 at 11:40 AM
molecularmicro.bsky.social
Rev1, a translation synthesis DNA polymerase of Candida albicans, binds to PCNA via its BRCT domain and plays a critical role in DNA damage response, but is dispensable for systemic candidiasis.
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
July 21, 2025 at 11:38 AM
molecularmicro.bsky.social
Candida albicans is a major fungal pathogen whose virulence is linked to filamentation. Its relative, C. dubliniensis, filaments less and rarely causes disease . Meza-Davalos et al. explore the regulatory basis for this difference.
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
July 21, 2025 at 11:36 AM
molecularmicro.bsky.social
Yeh, Kelly, and Misra show that mutations in RNA polymerase (rpoB) mimic the stringent response in the absence of (p)ppGpp and help maintain a healthy proteome under extreme growth temperatures and without molecular chaperones or proteases.
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
Characterization of a Novel RNA Polymerase Mutant of Escherichia coli That Confers Thermotolerance and Chaperone Independence
Protein homeostasis is perturbed at extremely high growth temperatures and without molecular chaperones or proteases. The protein synthesis-inhibiting branch of the stringent response involves elevat...
onlinelibrary.wiley.com
July 15, 2025 at 11:50 AM
molecularmicro.bsky.social
Becam et al. report unexpected anaerobic phenotypes associated with CueO, a periplasmic multicopper oxidase that contributes to copper resistance. This suggests that CueO may use an alternative electron acceptor in the absence of oxygen.
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
Role of the Multicopper Oxidase CueO in Copper Homeostasis Under Anaerobic Conditions in Enterobacteria
The periplasmic multicopper oxidase CueO oxidizes cuprous ions (Cu+) to cupric ions (Cu2+), a well-known process typically coupled with oxygen reduction. In this study, we report unexpected anaerobic...
onlinelibrary.wiley.com
July 15, 2025 at 11:47 AM
molecularmicro.bsky.social
Amidotransferases are essential for protein lipoylation in diverse eukaryotes. The selective inhibition by 8-bromooctanoate of amidotransferases from Capsaspora owczarzaki and Plasmodium falciparum reveals a conserved and druggable mechanism.
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
graphical abstract showing role of amidotransferase and inhibition by BrO.
July 15, 2025 at 11:43 AM
molecularmicro.bsky.social
Diverse fungal chitin synthases affect the growth and secondary metabolism of Monascus purpureus.
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
Multifaceted Roles of chs Genes in Regulating Cell Growth, Mycelial Morphology, Monascus Pigments and Citrinin Biosynthesis in Monascus purpureus
Chitin synthases exhibit distinct protein structures and functional diversity, resulting in differential regulatory effects on the growth and secondary metabolism of M. purpureus. An integrated appro...
onlinelibrary.wiley.com
July 15, 2025 at 11:38 AM
molecularmicro.bsky.social
Tiffany Zarrella et al. provide new insights into the role of the bacterial second messenger cyclic di-adenosine monophosphate (c-di-AMP) in maintaining cell envelope homeostasis in Streptococcus pneumoniae.
#MicroSky #cyclic-di-AMP
onlinelibrary.wiley.com/doi/10.1111/...
Cyclic Di‐AMP Affects Cell Membrane Integrity of Streptococcus pneumoniae
When Streptococcus pneumoniae produces low amounts of the second messenger cyclic di-AMP (c-di-AMP), they do not survive in the presence of competence-stimulating peptide (CSP), which is used for tra...
onlinelibrary.wiley.com
July 2, 2025 at 3:01 PM
molecularmicro.bsky.social
Xingru Chen et al. describe the role of the PhyK orphan kinease in Brucella obis. PhyK is a key regulator of cell envelope properties that can modulate host immune interactions.

#MicroSky #BLAST
onlinelibrary.wiley.com/doi/10.1111/...
An HWE‐Family Histidine Kinase Modulates Brucella Cell Envelope Properties and Host Innate Immune Response
The HWE-family histidine kinase PhyK regulates the cell envelope structure of the intracellular pathogen Brucella ovis and affects how the host immune system responds to this bacterium. Deletion of p...
onlinelibrary.wiley.com
July 2, 2025 at 2:56 PM
molecularmicro.bsky.social
Natalia de Miguel et al. present a new Review on how protozoan parasites communicate with each other and these processes affect social activities, physiological processes, and disease progression.
#MicroSky #protozoa #parasitology
onlinelibrary.wiley.com/doi/10.1111/...
Understanding Cell Communication Among Protozoan Parasites
Growing evidence points to the vital role of communication among parasites in coordinating behavior and promoting functional specialization. These interactions significantly impact drug resistance, c...
onlinelibrary.wiley.com
July 2, 2025 at 2:52 PM
molecularmicro.bsky.social
Li et al. characterize two ArsR-type transcriptional repressors (sHdrR and SoxR) that use conserved cysteines to sense sulfate sulfur!

#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
In Hyphomicrobium denitrificans Two Related Sulfane‐Sulfur Responsive Transcriptional Repressors Regulate Thiosulfate Oxidation and Have a Deep Impact on Nitrate Respiration and Anaerobic Biosyntheses
In Hyphomicrobium denitrificans, the use of thiosulfate as a supplemental electron donor is regulated by two homologous sulfane-sulfur responsive ArsR-type transcriptional repressors, sHdrR and SoxR,...
onlinelibrary.wiley.com
July 2, 2025 at 2:49 PM
Reposted by Molecular Microbiology Editors
cbarry3.bsky.social
Super useful - thanks all!! Just spent three hours playing with this - really nice to have that data accessible!!
June 25, 2025 at 5:02 PM
molecularmicro.bsky.social
Jinich et al. present an important new resource for the Mtb community!
The Mycobacterium tuberculosis Transposon Sequencing Database (MtbTnDB) is an interactive web resource summarizing 150+ published screens.
#MicroSky #Mycobacterium #tuberculosis
onlinelibrary.wiley.com/doi/10.1111/...
The Mycobacterium tuberculosis Transposon Sequencing Database (MtbTnDB): A Large‐Scale Guide to Genetic Conditional Essentiality
Mtb transposon sequencing database is a unified resource for exploring conditional gene essentiality in Mycobacterium tuberculosis. By standardizing over 150 transposon sequencing screens, it enables...
onlinelibrary.wiley.com
June 24, 2025 at 11:27 AM
molecularmicro.bsky.social
Stevens et al. describe the molecular basis for increased antibiotic tolerance in S. aureus COL: a prs mutation that reduces PRPP synthetase activity (leading to downregulation of pyrimidine, histidine, and tryptophan synthesis) plays a major role.
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
Staphylococcus aureus COL: An Atypical Model Strain of MRSA That Exhibits Slow Growth and Antibiotic Tolerance due to a Mutation in PRPP Synthetase
COL is an unusual “model” strain of Staphylococcus aureus that exhibits slow growth and multidrug antibiotic tolerance. This phenotype is primarily due to a mutation in Prs, which synthesizes the cor...
onlinelibrary.wiley.com
June 24, 2025 at 11:25 AM
molecularmicro.bsky.social
Sze et al. describe the role of FliS in post-transcriptional regulatory mechanisms governing flagellar assembly in B. burgdorferi, a spirochete with periplasmic flagella.

#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
The Flagellin‐Specific Chaperone FliS of Borrelia burgdorferi Controls the Cytoplasmic Pool of Flagellins at the Level of Translation Initiation, Secretion, and Proteolysis
FliS, a flagellin protein chaperon of the Lyme disease pathogen Borrelia burgdorferi, controls the cytoplasmic pool of flagellins (FlaB) at the level of translation initiation, secretion, and proteol...
onlinelibrary.wiley.com
June 13, 2025 at 4:39 PM
Reposted by Molecular Microbiology Editors
freddyfrischknecht.bsky.social
Nice cover @molmicrounihoh.bsky.social features Blastocrithidia nonstop, a trypanosomatid parasite with a non‐canonical genetic code. Associated paper by Slava Yurchenko, Julius Lukes and colleagues describes its genetic transformation: onlinelibrary.wiley.com/doi/10.1111/...
June 11, 2025 at 12:35 PM
molecularmicro.bsky.social
Experimental evolution studies reveal that Candida auris acquires amphotericin B resistance by diverse mechanisms; some dependent on and others independent of sterol content modulation.
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
Multi‐Omics Analysis of Experimentally Evolved Candida auris Isolates Reveals Modulation of Sterols, Sphingolipids, and Oxidative Stress in Acquired Amphotericin B Resistance
An overview of resistance mechanisms in two East Asian Candida auris isolates evolved in vitro. The A1.1 strain acquired resistance by enhancing oxidative stress responses through the AOX2, RTG3, and...
onlinelibrary.wiley.com
June 11, 2025 at 11:16 AM
molecularmicro.bsky.social
Dornes et al. show that Bacillus subtilis GpsB, a cell cycle regulator, can interact simultaneously with FlhG and PBP1. This supports a model in which a GpsB-FlhG interaction localizes flagella biosynthesis to regions of cell wall biosynthesis.
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
FlhG Cooperates With the Cell Cycle Regulator GpsB to Confine Peritrichous Flagella in B. subtilis
FlhG (orange, with activator helix in green) regulates peritrichous flagellation in Bacillus subtilis by interacting with the C-ring protein FliY (gray, N-terminus in red) and the cell wall regulator...
onlinelibrary.wiley.com
June 11, 2025 at 11:13 AM
molecularmicro.bsky.social
Yang and Swingle show that Pseudomonas syringae swimming motility is induced by proximity to other bacteria, leading to the formation of asymmetric colonies. This induced social behavior requires Moco.

#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
Pseudomonas syringae Socially Induced Swimming Motility Requires the Molybdenum Cofactor
Pseudomonas syringae swimming motility is induced by proximity to other species of bacteria, leading to the formation of asymmetric colonies. Upper panels show examples of P. syringae and mutants gro...
onlinelibrary.wiley.com
June 10, 2025 at 1:31 PM
molecularmicro.bsky.social
In Corynebacterium glutamicum, iron and heme homeostasis are controlled by the global regulators DtxR and HrrA. Here, these two overlapping regions are explored using chromatin affinity purification and sequencing (ChAP-Seq).
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
Genome‐Wide Analysis of DtxR and HrrA Regulons Reveals Novel Targets and a High Level of Interconnectivity Between Iron and Heme Regulatory Networks in Corynebacterium glutamicum
Iron is essential for life, yet excessive levels can quickly lead to cellular damage via oxidative stress, underscoring the need for global regulators of iron and heme homeostasis. In this study, we ...
onlinelibrary.wiley.com
June 10, 2025 at 11:42 AM
molecularmicro.bsky.social
Hill et al. use the Pseudomonas syringae ice nucleation protein (INP) surface display system to present Borrelia burgdorferi lipoproteins on the surface of E. coli to reveal conservation of complement inhibitory functions.
#MicroSky
onlinelibrary.wiley.com/doi/10.1111/...
Heterologous Surface Display Reveals Conserved Complement Inhibition and Functional Diversification of Borrelia burgdorferi Elp Proteins
A novel approach using the Pseudomonas syringae ice nucleation protein (INP) system to present Borrelia burgdorferi lipoproteins on the surface of Escherichia coli reveals conservation of complement ...
onlinelibrary.wiley.com
June 10, 2025 at 11:39 AM