We had a fantastic experience @jcb.org . The editors were fair and thoughtful, and the review process significantly strengthened both papers. 🙏 Would highly recommend for any junior faculty who dont want to mess around with lengthy review processes.

Second, Wyatt Miller clarifies the downstream signaling pathway that the "Don't Eat Me" signal CD47 uses to stop phagocytosis. A very satisfying answer to a major question we've had since I started my postdoc, with lots of interesting implications doi.org/10.1083/jcb....

Thank you!! Ill be at ASCB this year, we should catch up!

Thank you!!

Cool paper, congratulations!

🎉🎉🎉

I have been trying to ID the direct targets of these phosphatases since I started studying CD47 as a postdoc, but before this had only managed to find downstream targets. I am THRILLED to have a more satisfying answer.

With these clues, Wyatt pinpointed a specific cytoskeletal regulator that is dephosphorylated by SHP-phosphatases bound to SIRPA (CD47 receptor)... Vav1, a Rac GEF! Hyperactivating Vav1 or Rac meant macrophages could phagocytose cancer cells with CD47.

If macrophages managed to eat a target, it was through a slower sinking phagocytosis...

But when we added CD47, this type of phagocytosis failed...see how the macrophage keeps trying to extend membrane but doesn't quite make it

Macrophages usually reach out to phagocytose IgG coated targets... see the cell extending membrane out and around the bead

This supports the idea that target cell's biophysical properties regulate the decision to trogocytose instead of phagocytose, as Caitlin Cornell @fletcherlabucb.bsky.social proposed in their recent preprint: doi.org/10.1101/2024.12.02.626490

Thank you!!

Thank you! 😀

Thank you! 😀