Michael Grange
@michaelgrange.bsky.social
Group Leader at the Rosalind Franklin Institute. In situ imaging/cryo tomography of complex biological systems.
July 21, 2025 at 7:15 PM
So, what's next?
In the lab, we are already applying this workflow to different systems and tissues reproducibly.
Whatever cell biology questions drive your research, we hope you can implement our approach to gain molecular level insights within the native tissue environment.
In the lab, we are already applying this workflow to different systems and tissues reproducibly.
Whatever cell biology questions drive your research, we hope you can implement our approach to gain molecular level insights within the native tissue environment.
June 17, 2025 at 10:59 AM
So, what's next?
In the lab, we are already applying this workflow to different systems and tissues reproducibly.
Whatever cell biology questions drive your research, we hope you can implement our approach to gain molecular level insights within the native tissue environment.
In the lab, we are already applying this workflow to different systems and tissues reproducibly.
Whatever cell biology questions drive your research, we hope you can implement our approach to gain molecular level insights within the native tissue environment.
finally, using plasma allows you to reel in WHOPPERS you can see from space!
@cryingem.bsky.social 🎣🎣🎣🐟🐟🐟
@cryingem.bsky.social 🎣🎣🎣🐟🐟🐟
June 17, 2025 at 10:59 AM
finally, using plasma allows you to reel in WHOPPERS you can see from space!
@cryingem.bsky.social 🎣🎣🎣🐟🐟🐟
@cryingem.bsky.social 🎣🎣🎣🐟🐟🐟
Geometry was key - FIBs are flexible!
Having the ability to image the dendritic network from alternate perspectives using planar lift out gives us different insights.
In this case, molecular variabilities in the microtubule network could be teased out.
Having the ability to image the dendritic network from alternate perspectives using planar lift out gives us different insights.
In this case, molecular variabilities in the microtubule network could be teased out.
June 17, 2025 at 10:59 AM
Geometry was key - FIBs are flexible!
Having the ability to image the dendritic network from alternate perspectives using planar lift out gives us different insights.
In this case, molecular variabilities in the microtubule network could be teased out.
Having the ability to image the dendritic network from alternate perspectives using planar lift out gives us different insights.
In this case, molecular variabilities in the microtubule network could be teased out.
Assessing hundreds of sections, they optimised targeting, producing numerous snapshots of synaptic organisation in tissues.
June 17, 2025 at 10:59 AM
Assessing hundreds of sections, they optimised targeting, producing numerous snapshots of synaptic organisation in tissues.
Obviously, if you want native proteins the sample needs to be vitreous.
It was a heroic effort to establish proper vitreous cryo-fixation, testing many cryoprotectants - lifting out and imaging non vitreous samples is not fun!
Here's our blooper reel.
It was a heroic effort to establish proper vitreous cryo-fixation, testing many cryoprotectants - lifting out and imaging non vitreous samples is not fun!
Here's our blooper reel.
June 17, 2025 at 10:59 AM
Obviously, if you want native proteins the sample needs to be vitreous.
It was a heroic effort to establish proper vitreous cryo-fixation, testing many cryoprotectants - lifting out and imaging non vitreous samples is not fun!
Here's our blooper reel.
It was a heroic effort to establish proper vitreous cryo-fixation, testing many cryoprotectants - lifting out and imaging non vitreous samples is not fun!
Here's our blooper reel.
Proud to share our latest paper. doi.org/10.1016/j.cr...
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
June 17, 2025 at 10:59 AM
Proud to share our latest paper. doi.org/10.1016/j.cr...
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
When your colleague is french:
November 28, 2024 at 2:04 PM
When your colleague is french:
See, definitely helps!
November 19, 2024 at 8:44 PM
See, definitely helps!
Been thinking how to open up this account, and while science is fun, have you ever seen such epic side eye from a doggo just because you put a raincoat on her? #pupswithsass
November 17, 2024 at 6:48 PM
Been thinking how to open up this account, and while science is fun, have you ever seen such epic side eye from a doggo just because you put a raincoat on her? #pupswithsass