Max Stammnitz
@maxstammnitz.bsky.social
Postdoc at @crg.eu Barcelona,
previously PhD at Cambridge University
Genomics | Mutational Scanning | Molecular Evolution 🧬🌱
www.pyrisentinel.eu ⛰️🌊 www.puntseq.com
previously PhD at Cambridge University
Genomics | Mutational Scanning | Molecular Evolution 🧬🌱
www.pyrisentinel.eu ⛰️🌊 www.puntseq.com
These rich data allow us to fit thousands of dose-response curves.
With these we can systematically compare mutants’ key signalling parameters including basal phosphatase binding, hormone sensitivity and maximum response.
And there are lots of cool examples & correlations, surprises … 🔎🕵️💚
[5/7]
With these we can systematically compare mutants’ key signalling parameters including basal phosphatase binding, hormone sensitivity and maximum response.
And there are lots of cool examples & correlations, surprises … 🔎🕵️💚
[5/7]
June 2, 2025 at 1:36 PM
These rich data allow us to fit thousands of dose-response curves.
With these we can systematically compare mutants’ key signalling parameters including basal phosphatase binding, hormone sensitivity and maximum response.
And there are lots of cool examples & correlations, surprises … 🔎🕵️💚
[5/7]
With these we can systematically compare mutants’ key signalling parameters including basal phosphatase binding, hormone sensitivity and maximum response.
And there are lots of cool examples & correlations, surprises … 🔎🕵️💚
[5/7]
Now what does one get out of this??
A massive map of position-, amino acid-, and small-molecule concentration-dependent variant effects!
[4/7]
A massive map of position-, amino acid-, and small-molecule concentration-dependent variant effects!
[4/7]
June 2, 2025 at 1:36 PM
Now what does one get out of this??
A massive map of position-, amino acid-, and small-molecule concentration-dependent variant effects!
[4/7]
A massive map of position-, amino acid-, and small-molecule concentration-dependent variant effects!
[4/7]
To better understand this ABA receptor’s genetic encoding, we mutated every position to all 20 amino acids.
A glueable protein complementation assay (GluePCA) then allowed us to measure the relative binding strength of each receptor variant vs. phosphatase – at 12 different dosages of ABA.
[3/7]
A glueable protein complementation assay (GluePCA) then allowed us to measure the relative binding strength of each receptor variant vs. phosphatase – at 12 different dosages of ABA.
[3/7]
June 2, 2025 at 1:36 PM
To better understand this ABA receptor’s genetic encoding, we mutated every position to all 20 amino acids.
A glueable protein complementation assay (GluePCA) then allowed us to measure the relative binding strength of each receptor variant vs. phosphatase – at 12 different dosages of ABA.
[3/7]
A glueable protein complementation assay (GluePCA) then allowed us to measure the relative binding strength of each receptor variant vs. phosphatase – at 12 different dosages of ABA.
[3/7]
PYR/PYL receptors (grey) are key to plant water homeostasis and stress signalling. 💦🌱🌞
Abscisic acid ('ABA', green), is bound through a deep hydrophobic pocket. This triggers the allosteric closure of two loops which generate a new binding interface with response phosphatases (white).
[2/7]
Abscisic acid ('ABA', green), is bound through a deep hydrophobic pocket. This triggers the allosteric closure of two loops which generate a new binding interface with response phosphatases (white).
[2/7]
June 2, 2025 at 1:36 PM
PYR/PYL receptors (grey) are key to plant water homeostasis and stress signalling. 💦🌱🌞
Abscisic acid ('ABA', green), is bound through a deep hydrophobic pocket. This triggers the allosteric closure of two loops which generate a new binding interface with response phosphatases (white).
[2/7]
Abscisic acid ('ABA', green), is bound through a deep hydrophobic pocket. This triggers the allosteric closure of two loops which generate a new binding interface with response phosphatases (white).
[2/7]