Matyas Bubna-Litic
@matyasbl.bsky.social
Interested in mechanobiology, morphogenesis, organisers and synthetic embryo models.
Research Fellow in Mongera lab UCL | PhD in Mayor and Charras labs | YEN Committee
Research Fellow in Mongera lab UCL | PhD in Mayor and Charras labs | YEN Committee
Thanks! 🦓🐟 are great for that
November 21, 2025 at 6:08 PM
Thanks! 🦓🐟 are great for that
That's such a great book cover
October 4, 2025 at 10:05 PM
That's such a great book cover
Animal caps are back in fashion!
September 22, 2025 at 9:40 PM
Animal caps are back in fashion!
This worked for me:
155 mM NaCl, 10 mM Tris-HCl at pH 7.5, 50 mM NaBH4 > in refrigerator 2 days before use.
Use: After permeabilising apply AF-reducing agent overnight at 4˚C
It's possible to do it faster at room temp and higher NaBH4 conc but it's less gentle to the sample.
Hope it works!
155 mM NaCl, 10 mM Tris-HCl at pH 7.5, 50 mM NaBH4 > in refrigerator 2 days before use.
Use: After permeabilising apply AF-reducing agent overnight at 4˚C
It's possible to do it faster at room temp and higher NaBH4 conc but it's less gentle to the sample.
Hope it works!
August 10, 2025 at 10:51 PM
This worked for me:
155 mM NaCl, 10 mM Tris-HCl at pH 7.5, 50 mM NaBH4 > in refrigerator 2 days before use.
Use: After permeabilising apply AF-reducing agent overnight at 4˚C
It's possible to do it faster at room temp and higher NaBH4 conc but it's less gentle to the sample.
Hope it works!
155 mM NaCl, 10 mM Tris-HCl at pH 7.5, 50 mM NaBH4 > in refrigerator 2 days before use.
Use: After permeabilising apply AF-reducing agent overnight at 4˚C
It's possible to do it faster at room temp and higher NaBH4 conc but it's less gentle to the sample.
Hope it works!
Looks like an exciting meeting. Would you know if it's possible to attend online? Thanks
August 10, 2025 at 9:23 AM
Looks like an exciting meeting. Would you know if it's possible to attend online? Thanks
I'm not sure how bacteria on oysters are but in early Xenopus cells sodium borohydride works well to remove autofluorescence from sources like PFA fixation. There's a good protocol on CSH I can try look for it.
August 9, 2025 at 10:50 PM
I'm not sure how bacteria on oysters are but in early Xenopus cells sodium borohydride works well to remove autofluorescence from sources like PFA fixation. There's a good protocol on CSH I can try look for it.