If you study phospho-signaling, make sure you check out this work!

I'm thankful to Alexis and Judit for letting me be a part of this project and I'm really excited for others to try this reagent and method. Congrats to all the authors!

Alexis does a lot of amazing work benchmarking the reagent/method. She demonstrates how powerful it is by applying it to EGF-stimulated cells, uncovering over 1600 unique pY sites including many low abundance and previously uncharacterized sites with as little as 1 mg of peptide input per sample!

We estimate our approach for enriching pY-containing peptides is ~ 10x cheaper than using pY antibody magnetic beads and our magnetic enrichment reagent can easily be prepared by any lab that can grow E. coli!