Marco Payr
@marcopayr.bsky.social
PhD Candidate @embl.org | European Molecular Biology Laboratory | Duss and Hennig groups
#RNA structure and dynamics | #single-molecule | #biophysics | #structuralBiology | #translation | #biochemistry
#RNA structure and dynamics | #single-molecule | #biophysics | #structuralBiology | #translation | #biochemistry
🧵 (7/7) Combining different methodologies (smFRET, NMR & reporter gene assays) we developed a framework that can now be used to study similar processes in other biological systems! 🧬
hashtag#single-molecule hashtag#FRET hashtag#RNA hashtag#translation hashtag#Biophysics
hashtag#single-molecule hashtag#FRET hashtag#RNA hashtag#translation hashtag#Biophysics
April 11, 2025 at 12:26 PM
🧵 (7/7) Combining different methodologies (smFRET, NMR & reporter gene assays) we developed a framework that can now be used to study similar processes in other biological systems! 🧬
hashtag#single-molecule hashtag#FRET hashtag#RNA hashtag#translation hashtag#Biophysics
hashtag#single-molecule hashtag#FRET hashtag#RNA hashtag#translation hashtag#Biophysics
🧵 (6/7) Why this matters: Understanding these molecular mechanisms helps us grasp how cells regulate their genes through different complex assembly mechanisms.
April 11, 2025 at 12:26 PM
🧵 (6/7) Why this matters: Understanding these molecular mechanisms helps us grasp how cells regulate their genes through different complex assembly mechanisms.
🧵 (5/7) These mechanisms work together to achieve tight translation repression. And it’s the first time seeing this complex assembly in real-time!
April 11, 2025 at 12:26 PM
🧵 (5/7) These mechanisms work together to achieve tight translation repression. And it’s the first time seeing this complex assembly in real-time!
🧵 (4/7) Key Finding #4: A third protein (Hrp48) acts as a molecular "doorstop" - preventing RNA from displacing Sxl and Unr. 🚪
April 11, 2025 at 12:26 PM
🧵 (4/7) Key Finding #4: A third protein (Hrp48) acts as a molecular "doorstop" - preventing RNA from displacing Sxl and Unr. 🚪
🧵 (3/7) Key Finding #3: RNA-bound Sxl helps recruit another protein (Unr) 500x faster! Like a molecular speedway for protein assembly. 🏃♂️💨
April 11, 2025 at 12:26 PM
🧵 (3/7) Key Finding #3: RNA-bound Sxl helps recruit another protein (Unr) 500x faster! Like a molecular speedway for protein assembly. 🏃♂️💨
🧵 (2/7) Key Finding #2: We saw Sxl can slide along RNA between binding sites instead of just binding and falling off. This helps it stay attached longer. 🏄♂️
April 11, 2025 at 12:26 PM
🧵 (2/7) Key Finding #2: We saw Sxl can slide along RNA between binding sites instead of just binding and falling off. This helps it stay attached longer. 🏄♂️
🧵 (1/7) Key Finding #1: We discovered Sex-lethal (Sxl) alone, despite its high affinity, transiently samples its target sites
April 11, 2025 at 12:26 PM
🧵 (1/7) Key Finding #1: We discovered Sex-lethal (Sxl) alone, despite its high affinity, transiently samples its target sites
🧵 (7/7) Combining different methodologies (smFRET, NMR & reporter gene assays) we developed a framework that can now be used to study similar processes in other biological systems! 🧬
#single-molecule #FRET #RNA #translation #Biophysics
#single-molecule #FRET #RNA #translation #Biophysics
April 11, 2025 at 12:06 PM
🧵 (7/7) Combining different methodologies (smFRET, NMR & reporter gene assays) we developed a framework that can now be used to study similar processes in other biological systems! 🧬
#single-molecule #FRET #RNA #translation #Biophysics
#single-molecule #FRET #RNA #translation #Biophysics
🧵 (6/7) Why this matters: Understanding these molecular mechanisms helps us grasp how cells regulate their genes through different complex assembly mechanisms.
April 11, 2025 at 12:06 PM
🧵 (6/7) Why this matters: Understanding these molecular mechanisms helps us grasp how cells regulate their genes through different complex assembly mechanisms.
🧵 (5/7) These mechanisms work together to achieve tight translation repression. And it’s the first time seeing this complex assembly in real-time!
April 11, 2025 at 12:06 PM
🧵 (5/7) These mechanisms work together to achieve tight translation repression. And it’s the first time seeing this complex assembly in real-time!
🧵 (4/7) Key Finding #4: A third protein (Hrp48) acts as a molecular "doorstop" - preventing RNA from displacing Sxl and Unr. 🚪
April 11, 2025 at 12:06 PM
🧵 (4/7) Key Finding #4: A third protein (Hrp48) acts as a molecular "doorstop" - preventing RNA from displacing Sxl and Unr. 🚪
🧵 (3/7) Key Finding #3: RNA-bound Sxl helps recruit another protein (Unr) 500x faster! Like a molecular speedway for protein assembly. 🏃♂️💨
April 11, 2025 at 12:06 PM
🧵 (3/7) Key Finding #3: RNA-bound Sxl helps recruit another protein (Unr) 500x faster! Like a molecular speedway for protein assembly. 🏃♂️💨
🧵 (2/7) Key Finding #2: We saw Sxl can slide along RNA between binding sites instead of just binding and falling off. This helps it stay attached longer. 🏄♂️
April 11, 2025 at 12:06 PM
🧵 (2/7) Key Finding #2: We saw Sxl can slide along RNA between binding sites instead of just binding and falling off. This helps it stay attached longer. 🏄♂️
🧵 (1/7) Key Finding #1: We discovered Sex-lethal (Sxl) alone, despite its high affinity, transiently samples its target sites
April 11, 2025 at 12:06 PM
🧵 (1/7) Key Finding #1: We discovered Sex-lethal (Sxl) alone, despite its high affinity, transiently samples its target sites
Read our review for a deeper dive into the tools & insights shaping translation regulation research: doi.org/10.1042/BST2... #TranslationRegulation #RNA #SingleMolecule
Exploring the dynamics of messenger ribonucleoprotein-mediated translation repression
Translational control is crucial for well-balanced cellular function and viability of organisms. Different mechanisms have evolved to up- and down-regulate protein synthesis, including 3′ untranslated...
doi.org
November 29, 2024 at 3:24 PM
Read our review for a deeper dive into the tools & insights shaping translation regulation research: doi.org/10.1042/BST2... #TranslationRegulation #RNA #SingleMolecule
✴️ Single-molecule approaches bridge the gap between structural snapshots & real-world dynamics. As an example, smFRET can track dynamics of protein·RNA complex assembly and how it is coupled to RNA restructuring.
November 29, 2024 at 3:24 PM
✴️ Single-molecule approaches bridge the gap between structural snapshots & real-world dynamics. As an example, smFRET can track dynamics of protein·RNA complex assembly and how it is coupled to RNA restructuring.
🧪 How can we study this? Have a closer look at single-molecule fluorescence microscopy! Focusing on translation repression mechanisms, the method can reveal:
- Real-time assembly of complexes
- Rare intermediate states
- Kinetics of translation repression/activation with spatial context
- Real-time assembly of complexes
- Rare intermediate states
- Kinetics of translation repression/activation with spatial context
November 29, 2024 at 3:24 PM
🧪 How can we study this? Have a closer look at single-molecule fluorescence microscopy! Focusing on translation repression mechanisms, the method can reveal:
- Real-time assembly of complexes
- Rare intermediate states
- Kinetics of translation repression/activation with spatial context
- Real-time assembly of complexes
- Rare intermediate states
- Kinetics of translation repression/activation with spatial context
- Recruitment-blocking of the 43S PIC by 3’ UTR-bound proteins like Sex-lethal in Drosophila. 🧬
- microRNA-induced silencing complexes (miRISCs)
- eIF4E-binding proteins (4E-BPs) masking initiation factors
- microRNA-induced silencing complexes (miRISCs)
- eIF4E-binding proteins (4E-BPs) masking initiation factors
November 29, 2024 at 3:24 PM
- Recruitment-blocking of the 43S PIC by 3’ UTR-bound proteins like Sex-lethal in Drosophila. 🧬
- microRNA-induced silencing complexes (miRISCs)
- eIF4E-binding proteins (4E-BPs) masking initiation factors
- microRNA-induced silencing complexes (miRISCs)
- eIF4E-binding proteins (4E-BPs) masking initiation factors
🎯 Spotlight on the 3’ UTR! This region binds regulatory factors that can interfere with the 43S pre-initiation complex (PIC)—a key player during translation initiation. But how do RNA-binding proteins interfere with initiation? 🤔 Mechanisms of action can be:
November 29, 2024 at 3:24 PM
🎯 Spotlight on the 3’ UTR! This region binds regulatory factors that can interfere with the 43S pre-initiation complex (PIC)—a key player during translation initiation. But how do RNA-binding proteins interfere with initiation? 🤔 Mechanisms of action can be:
🔑 A key focus is translation repression: halting protein synthesis when & where needed. We explore different mechanisms that contribute to translation repression such as:
- RNA-binding proteins
- microRNAs
- RNA dynamics
- RNA-binding proteins
- microRNAs
- RNA dynamics
November 29, 2024 at 3:24 PM
🔑 A key focus is translation repression: halting protein synthesis when & where needed. We explore different mechanisms that contribute to translation repression such as:
- RNA-binding proteins
- microRNAs
- RNA dynamics
- RNA-binding proteins
- microRNAs
- RNA dynamics