Marie Held
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makaefer.bsky.social
Marie Held
@makaefer.bsky.social
Image analyst at the Centre for Cell Imaging, the University of Liverpool.

didyouknowimageanalysis.wordpress.com
CCI.liv.ac.uk
And we’re off. #CBIAS2025 6th Edition, first outside of the Crick but impressed by the localities at @wellcomecollection.bsky.social
@david-j-barry.bsky.social welcoming the community
November 24, 2025 at 10:23 AM
It’s that time of year that I make my pilgrimage to London for the #CBIAS2025 and its trilogy of Ps: presentations, posters and panel discussions. But first I did some exploring…
🎶 London calling to the faraway towns 🎶
November 24, 2025 at 6:55 AM
August 9, 2025 at 11:48 AM
August 8, 2025 at 9:16 PM
Ahhhhhhhh
August 4, 2025 at 7:12 PM
Long awaited annual leave commencing
August 2, 2025 at 8:52 AM
Lots of food for thought washed down with a cold pint after todays train the trainers 2025 CRICK Partnership & DAIM workshop #trainthetrainerBIA2025 @crick.ac.uk @ucl.ac.uk @imperialcollegeldn.bsky.social @martinjones.bsky.social @lankylaste.bsky.social @giuliapaci.bsky.social @dgaboriau.bsky.social
June 13, 2025 at 7:54 PM
June 13, 2025 at 2:26 PM
If you see this, post the most recent photo of yourself from your camera roll. No cheating!
May 6, 2025 at 9:19 PM
And just like that it’s all over. @zech-lab.bsky.social wrapping up the first Celldiscoverer 7 user group meeting hosted by @cci-liv.bsky.social part of @livuni-livsrf.bsky.social & @zeiss-microscopy.bsky.social Exciting, stimulating, fun meeting w lots of interesting discussions, knowledge exchange
April 25, 2025 at 11:46 AM
Different options to address the monstrous amount of data created by @zeiss-microscopy.bsky.social equipment #CD7-UGM inclusion Arivis Pro, Arivis Cloud and Arivis Hub.
April 25, 2025 at 10:53 AM
Last talk of the event by Alan Kidger from @zeiss-microscopy.bsky.social #CD7-UGM
April 25, 2025 at 10:48 AM
Summary: The CD7 is central for the more advanced imaging says. Certainly harnessing a lot of AI tools #CD7-UGM
April 25, 2025 at 10:30 AM
Another industry presentation showcasing the usage of the Celldiscoverer 7 within a commercial context. Presenter is James Dodgson from @astrazeneca.bsky.social #CD7-UGM
April 25, 2025 at 10:12 AM
Lots of useful features on the machine but there’s also lots to teach. However, opening the doors to new experiments and new workflows for users. #CD7-UGM ready correlative imaging widefield/LSM built in.
April 25, 2025 at 8:41 AM
Next up, a double act from @uniofnottingham.bsky.social presenting their experience imaging across scales on their two CD7 machines #CD7-UGM
April 25, 2025 at 8:34 AM
Reproducible imaging with minimised number of black pixels through guided acquisition. Low magnification overview image, detecting objects via analysis pipeline followed by detailed imaging of detected objects, e.g. though LSM. #CD7-UGM
April 25, 2025 at 8:17 AM
Staining with a panel of dyes; imaging and analysis within the Zeiss ecosystem #CD7-UGM
April 25, 2025 at 8:13 AM
We have lift off on day 2 of our #CD7-UGM kicking off with a presentation of Volker Döring from @zeiss-microscopy.bsky.social Talking about Advanced workflow for CD7 - from cell profiling to 3D cell Culture screening.
April 25, 2025 at 8:08 AM
The model does the heavy lifting sifting through a massive haystack of needles and less identified events need to be checked by the human in the loop. #CD7-UGM there are some patterns to be recognised within the populations of false positives. But also, what is truly binary in biology?
April 24, 2025 at 3:23 PM
Manual annotation is time consuming and not feasible for high throughput. Fluorescent probes also have negative side effects, mo channels mo problems. Also a lot of drugs are {highly} fluorescent. #CD7-UGM
AI to scale up rare event detection? Also scalable and sustainable? A big task…
April 24, 2025 at 3:14 PM
Proliferation, that does not take cell death into account. #CD7-UGM Added bu bonus with DAPI: quick and dirty cell cycle staging.
April 24, 2025 at 3:11 PM
Next up @cellmorphosero.bsky.social talking about quantification of cell cycle dynamics, high throughput style: stain the nuclei and count them? You - hopefully- know how many cells you’ve put in at the beginning. As always in science - it depends … #CD7-UGM cell counts to calculate cell …
April 24, 2025 at 3:08 PM
From cells to whole organisms - zebrafish - the microscope does most of the work. #CD7-UGM the ultimate aim is to count septin cages within different cell types within the fish.🐟 🔬🧑‍💻 currently tracking infection of transgenically fluorescently labelled cells and shigella.
April 24, 2025 at 2:56 PM
#CD7-UGM, CD7 with Airyscan was transformative for the lab’s work for screening compounds.
April 24, 2025 at 2:47 PM