Aggrecan is a key component of perineuronal nets (PNNs), extracellular structures that surround certain neuronal sub-populations and regulate neuroplasticity. This video shows aggrecan in an intact spinal cord, processed & imaged with our pipeline. #FluorescenceFriday

The New England biologist impulse to do science on a tick you pulled off of yourself – this is ours from this summer!

Neutrophil Extracellular Traps (NETs) are fibrous structures released by immune cells to bind and kill pathogens. In collaboration with Dr. Mark Looney's lab at @ucsfhealth.bsky.social, we used #DALISPIM to visualize 3D NET formation (magenta) triggered by inflammation in a mouse lung. 🫁

Glomeruli, the filtering parts of nephrons, are visible in cyan in this mouse kidney section imaged with DALISPIM. In mice, kidneys have over 10,000 nephrons!

Our new open-top #lightsheet microscope's unique design allowed us to image this 1-mm thick sample directly in a 12-well plate.

Dr. Khalil Ramadi's lab at NYU Abu Dhabi used the LifeCanvas pipeline to develop CORAL, an ingestible device to non-invasively sample the small intestine #microbiome. The #SmartSPIM video below shows blood vessels (pink) & epithelial cells (blue) in rat duodenum. Check out the paper: bit.ly/3JncRfA

At first, these may look like individually mounted samples. But all 12 datasets were captured at once, directly in a 12-well plate, using our new open-top #DALISPIM light sheet microscope.🔬

Vasculature (CD31) in magenta, parvalbumin cells (endogenous tdTomato) in yellow, and nuclei (DAPI) in cyan.