This is actually my specialty. We've got an array of tricks and tools for working with DNA sequences that E. coli hates. Happy to discuss more.

In America, they have a Mason-Dixon Line. In Canada, it’s the Swiss Chalet-St-Hubert Line.

Ever run your plasmids through plannotate.barricklab.org ? Those blank spaces often hold the ghosts of projects passed. I've discovered unannotated strong bacterial promoters pointing right at my potentially toxic ORFs.

There is no justification for a higher bar of fees/taxes/anti-gentrification policy for apartments. Low density is the most gentrifying build environment in a city, lived in by the richest. Higher density is what lower income and less wealthy people live in.