We used TARDIS to look at the in vivo movement of RNA polymerase, and show that we can easily lower the measurement time by a factor of ~5. This was limited by the required high-density localization, NOT by TARDIS performance. (5/8)

This concept accurately obtains either an analytically fitted distribution (anything is possible here), or a jump-distance histogram. It is far more robust than any tracking algorithm (at high complexity), and constantly surprised us with its robustness. (3/8)

TARDIS is a conceptually new method to perform single-particle tracking analysis: all localizations are compared to themselves with a time-shift. The intraparticle population is separated from the interparticle distribution by observing time delays longer than track lengths: (2/8)