@kimboonekamp.bsky.social
(6/6) Our new pipeline now allows us to dissect how such a complex multicellular system like a budding intestinal organoid achieves its mechanical stability. Thanks a lot to @omdrozdowski.bsky.social and @ulrichschwarz.bsky.social for the amazing collaboration.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Fully three-dimensional force inference in intestinal organoids reveals ratchet-like bud stabilization
The intestinal epithelium in vertebrates has a characteristic architecture of protruding villi and receding crypts that enables nutrient absorption and cellular turnover. Intestinal organoids recapitu...
www.biorxiv.org
April 10, 2025 at 12:26 PM
(6/6) Our new pipeline now allows us to dissect how such a complex multicellular system like a budding intestinal organoid achieves its mechanical stability. Thanks a lot to @omdrozdowski.bsky.social and @ulrichschwarz.bsky.social for the amazing collaboration.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
(5/6) Moreover, we found that luminal pressure decreases and basal line tensions increase during budding, suggesting that budding has to be facilitated and stabilized by different mechanical processes.
April 10, 2025 at 12:26 PM
(5/6) Moreover, we found that luminal pressure decreases and basal line tensions increase during budding, suggesting that budding has to be facilitated and stabilized by different mechanical processes.
(4/6) Looking at different time points, we found that apico-basal tension differences increase after 48h, when budding occurs, as expected. However, we also found that afterwards they decrease again, suggesting a ratchet-like irreversibility of budding.
April 10, 2025 at 12:26 PM
(4/6) Looking at different time points, we found that apico-basal tension differences increase after 48h, when budding occurs, as expected. However, we also found that afterwards they decrease again, suggesting a ratchet-like irreversibility of budding.
(3/6) The method was verified with simulated data based on the bubbly vertex model.
April 10, 2025 at 12:26 PM
(3/6) The method was verified with simulated data based on the bubbly vertex model.
(2/6) Image stacks of mouse intestinal organoids were segmented using the statistics of point clouds to obtain a fully 3D reconstruction of the cells, including all surface normals. Then force balance was used to infer surface and line tensions.
April 10, 2025 at 12:26 PM
(2/6) Image stacks of mouse intestinal organoids were segmented using the statistics of point clouds to obtain a fully 3D reconstruction of the cells, including all surface normals. Then force balance was used to infer surface and line tensions.