Kent Boles
@kentboles.bsky.social
Synthetic biologist working on cell-free DNA
www.bespoke.bio
www.bespoke.bio
October 28, 2025 at 1:49 PM
Now that's a hairpin!
October 11, 2025 at 9:53 PM
Now that's a hairpin!
I didn't see a patent app.
October 2, 2025 at 12:50 AM
I didn't see a patent app.
Original paper www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
August 25, 2025 at 8:02 PM
Original paper www.pnas.org/doi/10.1073/...
Roger Tsien's work on FPs was invaluable. The original pub where they evolved mRFP1 is a great read. Here's the associated method. www.tsienlab.ucsd.edu/Publications...
www.tsienlab.ucsd.edu
August 25, 2025 at 6:17 PM
Roger Tsien's work on FPs was invaluable. The original pub where they evolved mRFP1 is a great read. Here's the associated method. www.tsienlab.ucsd.edu/Publications...
You can upload a document with up to 0.5M words to notebooklm.google.com
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notebooklm.google.com
August 14, 2025 at 9:37 PM
You can upload a document with up to 0.5M words to notebooklm.google.com
Did you say Falco? youtu.be/8-bgiiTxhzM?...
Too bad it's not a rocker for branding.
Too bad it's not a rocker for branding.
Falco - Der Kommissar (U.S. Official Video) (VOD)
YouTube video by FalcoVEVO
youtu.be
August 14, 2025 at 3:16 PM
Did you say Falco? youtu.be/8-bgiiTxhzM?...
Too bad it's not a rocker for branding.
Too bad it's not a rocker for branding.
It's nice the lids are shared. You could develop circulation, light or sensors on the lids that could fit multiple depth / volumes.
August 12, 2025 at 1:07 AM
It's nice the lids are shared. You could develop circulation, light or sensors on the lids that could fit multiple depth / volumes.
My Walmart has a pack for $6
July 13, 2025 at 7:41 PM
My Walmart has a pack for $6
I use this one in my garden. It's quite the zoo of species. plantrevolution.com/products/sol...
Plant Success Organics Soluble® Mycorrhizae - with Bacteria, Kelp and Humic Acids
Plant Success Organics Soluble™ will enhance established plants with beneficial soil microbes. Description It contains mycorrhizae, bacteria and trichoderma. It is easily watered in and will instantly...
plantrevolution.com
July 13, 2025 at 2:00 AM
I use this one in my garden. It's quite the zoo of species. plantrevolution.com/products/sol...
It sounds backwards, but institutes could pay a subscription for access so their members could publish for free.
July 10, 2025 at 4:26 AM
It sounds backwards, but institutes could pay a subscription for access so their members could publish for free.
Do you have the patent?
July 4, 2025 at 12:13 AM
Do you have the patent?
I agree, but you're talking about a 50% pay cut, at least. Also, one can expect automation to slowly take over the simple work. There's always a niche for hand-crafted, but industrialization usually takes over. Oligos and gene fragments are mostly automated...
June 30, 2025 at 4:29 AM
I agree, but you're talking about a 50% pay cut, at least. Also, one can expect automation to slowly take over the simple work. There's always a niche for hand-crafted, but industrialization usually takes over. Oligos and gene fragments are mostly automated...
Tolerance for variability has to be taught early on. Had a PhD physicist in a group, the bio lab meeting would frustrate him w all the bad design. I finally explained that biology is a stochastic system with millions of doors opened and shut leading to our system. He was depressed after that.
June 30, 2025 at 4:20 AM
Tolerance for variability has to be taught early on. Had a PhD physicist in a group, the bio lab meeting would frustrate him w all the bad design. I finally explained that biology is a stochastic system with millions of doors opened and shut leading to our system. He was depressed after that.
Design is cheap and scales since it's digital. Build and test are the hard parts being largely analog. We are getting better at automating, but the methods aren't universal. Hands on still competes since it's so versatile, but it doesn't scale and takes years to learn. Where to retrain and enter?
June 30, 2025 at 4:11 AM
Design is cheap and scales since it's digital. Build and test are the hard parts being largely analog. We are getting better at automating, but the methods aren't universal. Hands on still competes since it's so versatile, but it doesn't scale and takes years to learn. Where to retrain and enter?
Sorry, 50bp to keep the promoter from being active on the arm.
June 3, 2025 at 3:49 AM
Sorry, 50bp to keep the promoter from being active on the arm.
I've done this doing serial knockouts in a haploid yeast. 500bp on one arm and only 50bp to preserve the promoter. Yeast are fairly recombinogenic, though. In eukaryotes, NHEJ competes so you ko those genes or inhibit ligases, etc.
June 3, 2025 at 3:31 AM
I've done this doing serial knockouts in a haploid yeast. 500bp on one arm and only 50bp to preserve the promoter. Yeast are fairly recombinogenic, though. In eukaryotes, NHEJ competes so you ko those genes or inhibit ligases, etc.
Yes, I'm sure you'll find an activating mutation. There are quite a few in the literature since those variants lend themselves to structural studies.
May 23, 2025 at 5:03 AM
Yes, I'm sure you'll find an activating mutation. There are quite a few in the literature since those variants lend themselves to structural studies.
Oh good, I wonder why it's so active?
May 23, 2025 at 4:55 AM
Oh good, I wonder why it's so active?
I meant to type autoclave. Weird.
I aliquot SOC into 50ml tubes and autoclave them. The polypropylene is usually fine. My latest batch of tubes from China had some diffent plastic in the lids and they melted.
I aliquot SOC into 50ml tubes and autoclave them. The polypropylene is usually fine. My latest batch of tubes from China had some diffent plastic in the lids and they melted.
May 5, 2025 at 12:06 AM
I meant to type autoclave. Weird.
I aliquot SOC into 50ml tubes and autoclave them. The polypropylene is usually fine. My latest batch of tubes from China had some diffent plastic in the lids and they melted.
I aliquot SOC into 50ml tubes and autoclave them. The polypropylene is usually fine. My latest batch of tubes from China had some diffent plastic in the lids and they melted.
I'd like to be there when you open the microwave
Bill Hader Eating Popcorn GIF
ALT: Bill Hader Eating Popcorn GIF
media.tenor.com
May 4, 2025 at 6:41 PM
I'd like to be there when you open the microwave