Here's your (fluid) ounce, and just one tube is a tablespoon!

I think this organoid is upset with me 😠

Cool work from Sarah Bray's lab at Cambridge looking at Notch-induced transcription hubs:
www.biorxiv.org/content/10.1...

from Fig 5: hubs are stabilized/strengthened when initiation is inhibited and diminished when P300 is inhibited.

Hey NC/Triangle area folks: registration is now open for the annual Chromatin and Epigenetics Symposium at UNC on March 6th! This year's program will feature a keynote talk from Cigall Kadoch and short talks selected from abstracts. Click to register: unclineberger.org/event/2025-c...

Choose 20 books that have stayed with you or influenced you. No explanations, no reviews, just covers. #Books #BookSky #BookChallenge

Days 7+8
The Master and Margarita - Mikhail Bulgakov
The Satanic Verses - Salman Rushdie

Choose 20 books that have stayed with you or influenced you. No explanations, no reviews, just covers. #Books #BookSky #BookChallenge

Day 5+6
A memory called empire - Arkady Martine
A desolation called peace - Arkady Martine

Choose 20 books that have stayed with you or influenced you. No explanations, no reviews, just covers. #Books #BookSky #BookChallenge

Day 4
The New Wilderness - Diane Cook

Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers. #Books #BookSky #BookChallenge

Day 3
Children of Memory - Adrian Tchaikovsky

Day 2
Slow River by Nicola Griffith

Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

#Books
#BookSky
#BookChallenge

Day 1
The Sparrow by Mary Doria Russell

Finally, we tested the HAT/HDAC role in this. TSA treatment blocked/masked the increase in acetylation whereas p300 inhibition didn't prevent the triptolide effect. Thus, we propose that HDAC activity at TSSs is coordinated by RNAP2 and txn initiation

Blocking pause release with Flavopiridol did not trigger the same increase in acetylation, suggesting that loss of RNAP2 and initiation were the critical steps here. Indeed, using a HCT116-AID-RPB1 cells to degrade RNAP2 yielded similar results as triptolide

Not just K27ac. Every acetyl mark we checked was increased at active TSSs. H2AZ was also increased at TSSs, but H3K4me3 and ATAC accessibility are unchanged

This was all done in T47D A12 breast cancer cells, so we then confirmed these patterns of K27ac, K4me3, and H2AZ in 7 additional cell lines

We used triptolide to test whether transcription was required for hormone-induced histone acetylation. It was not... in fact, acetylation was increased following inhibition of initiation