Jan Binovsky
@jbinovsky.bsky.social
🇸🇰PhD student at Plevka Lab, CEITEC_Brno🇨🇿 | biochemistry & structural biology 🧬🧫🧪🔬
December 17, 2024 at 9:40 AM
The tail sheath contraction pushes the tail tube's tip into the host cytoplasm, where the genome is ejected 🧬 and infection occurs.
Our structural study of phi812 cell wall binding opens up the possibility of designing phi812 to target specific #SAureus strains 🎯
9/11
Our structural study of phi812 cell wall binding opens up the possibility of designing phi812 to target specific #SAureus strains 🎯
9/11
September 23, 2024 at 4:36 PM
The tail sheath contraction pushes the tail tube's tip into the host cytoplasm, where the genome is ejected 🧬 and infection occurs.
Our structural study of phi812 cell wall binding opens up the possibility of designing phi812 to target specific #SAureus strains 🎯
9/11
Our structural study of phi812 cell wall binding opens up the possibility of designing phi812 to target specific #SAureus strains 🎯
9/11
Changes in the baseplate arms' positions are relayed to the wedge modules and tail sheath initiator proteins, causing their iris-like expansion 👁️ and triggering a gradual tail sheath contraction. This 🌊-like process drives the tail tube through the host cell wall 💉.
8/11
8/11
September 23, 2024 at 4:32 PM
Changes in the baseplate arms' positions are relayed to the wedge modules and tail sheath initiator proteins, causing their iris-like expansion 👁️ and triggering a gradual tail sheath contraction. This 🌊-like process drives the tail tube through the host cell wall 💉.
8/11
8/11
The transformation of tripod complexes frees the hub protein domains to degrade peptidoglycan ✂️ and facilitate penetration of the tail tube through the cell membrane.
But how does the tail tube move towards the cell❓
7/11
But how does the tail tube move towards the cell❓
7/11
September 23, 2024 at 4:30 PM
The transformation of tripod complexes frees the hub protein domains to degrade peptidoglycan ✂️ and facilitate penetration of the tail tube through the cell membrane.
But how does the tail tube move towards the cell❓
7/11
But how does the tail tube move towards the cell❓
7/11
The central spike protein of phi812 protrudes 22 nm from the baseplate and degrades teichoic acids✂️, enabling the tripod complexes to anchor the phage on the cell wall ⚓️.
6/11
6/11
September 23, 2024 at 4:28 PM
The central spike protein of phi812 protrudes 22 nm from the baseplate and degrades teichoic acids✂️, enabling the tripod complexes to anchor the phage on the cell wall ⚓️.
6/11
6/11
The tripod complexes undergo an intra-molecular change. They then “hold hands” together 🤝, stabilizing the post-attachment (contracted) baseplate structure.
But how can they reach peptidoglycan covered by teichoic acids❓
5/11
But how can they reach peptidoglycan covered by teichoic acids❓
5/11
September 23, 2024 at 4:26 PM
The tripod complexes undergo an intra-molecular change. They then “hold hands” together 🤝, stabilizing the post-attachment (contracted) baseplate structure.
But how can they reach peptidoglycan covered by teichoic acids❓
5/11
But how can they reach peptidoglycan covered by teichoic acids❓
5/11
Upon interaction with the host, the baseplate changes structure, starting with the arms 🦾. These changes include reorienting receptor-binding proteins and tripod complexes that bind to 🦠 via high-avidity interactions with teichoic acids and peptidoglycan.
4/11
4/11
September 23, 2024 at 4:25 PM
Upon interaction with the host, the baseplate changes structure, starting with the arms 🦾. These changes include reorienting receptor-binding proteins and tripod complexes that bind to 🦠 via high-avidity interactions with teichoic acids and peptidoglycan.
4/11
4/11
We used #cryoEM #Xray #NMR #AlphaFold to reconstruct and interpret the phi812 baseplate and tail in detail 🔎. The baseplate of phi812 comprises a core, wedge modules, and baseplate arms that carry receptor-binding proteins 1 & 2 and large tripod complexes.
3/11
3/11
September 23, 2024 at 4:23 PM
We used #cryoEM #Xray #NMR #AlphaFold to reconstruct and interpret the phi812 baseplate and tail in detail 🔎. The baseplate of phi812 comprises a core, wedge modules, and baseplate arms that carry receptor-binding proteins 1 & 2 and large tripod complexes.
3/11
3/11
Contractile-tailed phages use baseplates to attach to 🦠 and initiate infection. How phages target and penetrate Gram+ 🦠 is poorly understood.
Meet phage phi812, a #PhageTherapy contender infecting #AntibioticResistant S. aureus.
2/11
Meet phage phi812, a #PhageTherapy contender infecting #AntibioticResistant S. aureus.
2/11
September 23, 2024 at 4:20 PM
Contractile-tailed phages use baseplates to attach to 🦠 and initiate infection. How phages target and penetrate Gram+ 🦠 is poorly understood.
Meet phage phi812, a #PhageTherapy contender infecting #AntibioticResistant S. aureus.
2/11
Meet phage phi812, a #PhageTherapy contender infecting #AntibioticResistant S. aureus.
2/11
Just finished registration for the Gordon Research Seminar (GRS), complementing the conference (GRC) on 3D #ElectronMicrocopy 🔥
Looking forward to attending the GRC/S for the first time! Am I gonna meet someone from my BlueSky bubble? 🙌
Looking forward to attending the GRC/S for the first time! Am I gonna meet someone from my BlueSky bubble? 🙌
March 21, 2024 at 2:01 PM
Just finished registration for the Gordon Research Seminar (GRS), complementing the conference (GRC) on 3D #ElectronMicrocopy 🔥
Looking forward to attending the GRC/S for the first time! Am I gonna meet someone from my BlueSky bubble? 🙌
Looking forward to attending the GRC/S for the first time! Am I gonna meet someone from my BlueSky bubble? 🙌
POV: renaming protein chains in your model. Symmetry mates not included.
February 7, 2024 at 12:00 PM
POV: renaming protein chains in your model. Symmetry mates not included.