Thanks!

Yeah, we thought same--that it might be something similar to what happens with Vero passaging. It's an epithelial cell organoid (low cell growth) with replication >2 months. However, there's TMPRSS2 expression and R682W is very consistently gained in our BA.5 but not WA-1 replicates 🤷

Same. I needed to build better writing habits before I got a ton of value from it

We are getting evolution of an FCS weakening mutation in a persistent infection model. Noticed anything about R682W specifically?

No, haven’t seen any. I vaguely remember a few professional level dancers posting about lingering issues years ago on Instagram but that’s the extent

Unfortunately, persistent SARS-CoV-2 seems to be confined to tissues. If a good cell/animal model for SARS-CoV-2 persistence was available, parameters from models could be adapted to understand dynamics and feedback loops required for persistence. Until then, the focus is understandable.

Will the choroid plexus be sampled alongside other CNS tissue being examined? Despite substantial evidence for potential infection, most non-LC autopsy studies skip sampling it.

I've actually been learning Rust over this past year in large part because I kept seeing you post about it on twitter lol. Your evangelizing is working

People keep telling me I should monetize it but I have no idea where I want to go with this now. Ballet was 10 years ago now. As I get more into medicine and try to wrap up my PhD, I'm finding I have less and less time and I'm finding it hard to let it die.

I love the quote "You can just do things." This was the biggest shift in thinking from this. I felt like this was my first step from being a consumer to a do-er.

In roughly 3 years, I was a leader in this niche thing of ballet analytics because no one else really did it. I've met tons of people online and it's helped my science career directly. The project website popped off and receives way more traffic than any journal article I've written (so far 🙃).

Once I learned R, I decided I was going to study ballet trajectories as a side project. To be honest, it felt trivial at the time. I learned shiny, the tidyverse, and Quarto because of it.

I just kept doing it for years and people started to notice. I shared the data freely, making some friends.

After some injuries, I read the signals--moved back home, loafed around for a year, and went to college. Yet, I had this idea that I had mismanaged my short ballet career. When I was in, I didn't research companies well and generally had no real sense of what my chances of progression were.