Jana Kroll
@janakroll.bsky.social
Postdoc at MDC Berlin
cryo-electron tomography, neuronal synaptic transmission, optogenetics
cryo-electron tomography, neuronal synaptic transmission, optogenetics
Thank you so much! 😊
October 1, 2025 at 4:08 PM
Thank you so much! 😊
In the left image, I see a queen with a crown🫅 But I guess that's not what you meant? 😅
May 13, 2025 at 5:46 PM
In the left image, I see a queen with a crown🫅 But I guess that's not what you meant? 😅
The video shows more than 50 snapshots of vesicle fusion events in synapses, sorted by fusion progression 😎
February 15, 2025 at 8:16 PM
The video shows more than 50 snapshots of vesicle fusion events in synapses, sorted by fusion progression 😎
... but also between vesicles and cytoskeletal fibers likely resembling actin.
February 12, 2025 at 10:34 PM
... but also between vesicles and cytoskeletal fibers likely resembling actin.
We did not only observe filamentous connectors at vesicles but also between vesicles and fusion events....
February 12, 2025 at 10:32 PM
We did not only observe filamentous connectors at vesicles but also between vesicles and fusion events....
We further quantified distributions and tethering of synaptic vesicles at the active zone. Tether numbers closely correlated to the distance between vesicle and membrane, but not to their respective fusion readiness.
February 12, 2025 at 10:31 PM
We further quantified distributions and tethering of synaptic vesicles at the active zone. Tether numbers closely correlated to the distance between vesicle and membrane, but not to their respective fusion readiness.
Overall, we analyzed more than 400 tomograms of synapses and identified more than 50 vesicle fusion intermediates. We classified and morphometrically characterized them.
February 12, 2025 at 10:23 PM
Overall, we analyzed more than 400 tomograms of synapses and identified more than 50 vesicle fusion intermediates. We classified and morphometrically characterized them.
Beyond that, we were able to correlate the cryo-fluorescent signal of iGluSnFR3 with a tomogram of a neuronal synapse. The arrowhead points to a putative vesicle fusion event.
February 12, 2025 at 10:22 PM
Beyond that, we were able to correlate the cryo-fluorescent signal of iGluSnFR3 with a tomogram of a neuronal synapse. The arrowhead points to a putative vesicle fusion event.
We confirmed successful stimulation via cryo-confocal microscopy of the glutamate sensor iGluSnFR3. With this experiment, we also proved that fast fluorescent biosensors can be used under cryogenic conditions.
February 12, 2025 at 10:20 PM
We confirmed successful stimulation via cryo-confocal microscopy of the glutamate sensor iGluSnFR3. With this experiment, we also proved that fast fluorescent biosensors can be used under cryogenic conditions.
We developed a workflow for time-resolved in situ cryo-ET by coupling optogenetics with subsequent plunge freezing of neurons. Our setup allowed us to stimulate neurons only a few milliseconds before cryofixation.
February 12, 2025 at 10:14 PM
We developed a workflow for time-resolved in situ cryo-ET by coupling optogenetics with subsequent plunge freezing of neurons. Our setup allowed us to stimulate neurons only a few milliseconds before cryofixation.
First and foremost, thank you @icecoldsynapse.bsky.social, @mohsensadeghi.bsky.social, @mishakudryashev.bsky.social, Christoph, Lia, Gosia, Thiemo, Magdalena and Christian for your contributions!
February 12, 2025 at 10:09 PM
First and foremost, thank you @icecoldsynapse.bsky.social, @mohsensadeghi.bsky.social, @mishakudryashev.bsky.social, Christoph, Lia, Gosia, Thiemo, Magdalena and Christian for your contributions!