Huiqing Zhou
@huiqingzhou.bsky.social
Pinned
Huiqing Zhou
@huiqingzhou.bsky.social
· Nov 4
Directed evolution of an m6A eraser for site-selective epitranscriptome editing
N6-methyladenosine (m6A) is a critical RNA modification, but tools to manipulate it in cells remain limited. Current m6A demethylases (FTO and ALKBH5) exhibit low catalytic efficiency, hindering their...
www.researchsquare.com
Excited to share the most recent preprint from our lab. We report a directed evolution platform for m6A erasers and an FTO variant with 13-fold enhanced catalytic efficiency. The FTO variant shows promoted site-specific editing efficiency of m6A in human cells. www.researchsquare.com/article/rs-7...
Excited to share the most recent preprint from our lab. We report a directed evolution platform for m6A erasers and an FTO variant with 13-fold enhanced catalytic efficiency. The FTO variant shows promoted site-specific editing efficiency of m6A in human cells. www.researchsquare.com/article/rs-7...
Directed evolution of an m6A eraser for site-selective epitranscriptome editing
N6-methyladenosine (m6A) is a critical RNA modification, but tools to manipulate it in cells remain limited. Current m6A demethylases (FTO and ALKBH5) exhibit low catalytic efficiency, hindering their...
www.researchsquare.com
November 4, 2025 at 2:41 PM
Excited to share the most recent preprint from our lab. We report a directed evolution platform for m6A erasers and an FTO variant with 13-fold enhanced catalytic efficiency. The FTO variant shows promoted site-specific editing efficiency of m6A in human cells. www.researchsquare.com/article/rs-7...
A follow-up interview with the editor @cp-cellchembiol.bsky.social to share our journeys and perspectives, along with our recent paper on MapID-tRNA-seq.
May 16, 2025 at 3:59 AM
A follow-up interview with the editor @cp-cellchembiol.bsky.social to share our journeys and perspectives, along with our recent paper on MapID-tRNA-seq.
Happy to share a paper from our group on "MapID-tRNA-seq" for confidently identifying modifications by RT mutation signatures in human tRNAs. This method will enable future discoveries of modifications' occurrence and functions in tRNA biology and diseases. www.cell.com/cell-chemica...
MapID-based quantitative mapping of chemical modifications and expression of human transfer RNA
tRNA chemical modifications play critical roles in biology and diseases. Tepe et al.
report MapID-tRNA-seq to map m1A, m3C, and multiple bulky modifications in tRNAs from
HEK293T and breast cancer-rel...
www.cell.com
May 16, 2025 at 3:58 AM
Happy to share a paper from our group on "MapID-tRNA-seq" for confidently identifying modifications by RT mutation signatures in human tRNAs. This method will enable future discoveries of modifications' occurrence and functions in tRNA biology and diseases. www.cell.com/cell-chemica...