We are excited to report:
Cerebral blood flow is modulated by astrocytic cAMP elevation independently of IP3R2-mediated Ca2+ signaling in mice
Vittani et al. PNAS (2025)
pnas.org/doi/10.1073/...

Membrane AAV jelly beans deposited at addgene.org
pAAV-CAG-Lck-mTagBFP2/mCerulean3/mStayGold/mScarlet3

Releasing several AAV plasmids used in the Alb-mScarlet-SpyTag003 knockin mouse study (doi.org/10.1101/2025...).

Addgene:
addgene.org/browse/artic...

Our red fluorescent blood mouse is available from RIKEN BRC (RBRC12137)
knowledge.brc.riken.jp/resource/ani...
It's highly versatile!
Vittani, Lee, Wang, Hiraoka et al (2025) doi.org/10.1101/2025...

Suppl. Video 5. Prefrontal cortex (red: alb-mScarlet-SpyTag003, green: neuronal GCaMP; kudos to @thomasbienvenu.bsky.social )
www.biorxiv.org/content/10.1...

Suppl. Video 4. Nucleus accumbens (compressed)

Vittani, Lee, Wang, Hiraoka, et al.
KI mouse expressing red fluorescent blood
In situ SpyTag/Catcher attachment of biosensor
ECS SpyCatcher to capture BBB leak
doi.org/10.1101/2025...
We hope this method is useful to a wide range of the biology community.