Sam Golden
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goldenneuron.bsky.social
Sam Golden
@goldenneuron.bsky.social
Neuroscientist, interested in all things motivation. UW Neurobiology and Biophysics. UW NAPE Center. Personal, unaffiliated, account.

https://www.goldenneurolab.com/
ArgiNLS in SHIELD cleared sample on LSFM.
YouTube video by Sam Golden
youtu.be
December 12, 2024 at 11:53 PM
Clip from her talk: AAVretro-ArgiNLS-DIO-oScarlet in red, AAV-ArgiNLS-GFP in green (both in the NAc) of a vlgut2-Cre transgenic. Imaged at 1.6x with a SmartSPIM LSFM. Rendered with Arivis.

This is the native (non stained) signal following SHIELD clearing.

Constructs at www.addgene.org/Sam_Golden/
December 12, 2024 at 11:49 PM
A simple one, but we had never tried HA Tag with iDisco+ before. Worked well!
December 6, 2024 at 3:21 PM