Fabio Lolicato
@fabiololicato.com
Group Leader at the Heidelberg University Biochemistry Center (BZH)
A computational physicist lost into a biochemistry Lab.
Find me on Mastodon:
@[email protected]
https://fabiololicato.com
A computational physicist lost into a biochemistry Lab.
Find me on Mastodon:
@[email protected]
https://fabiololicato.com
@bzh-hd.bsky.social @uniheidelberg.bsky.social @sfb1638.bsky.social @manpreet0700.bsky.social @walter-nickel-bzh.bsky.social
September 16, 2025 at 2:57 PM
Too generous :) Thanks!
July 21, 2025 at 10:31 PM
Too generous :) Thanks!
This work is the result of a shared @dfg.de grant between @walter-nickel-bzh.bsky.social and me, carried out by our joint PhD student @manpreet0700.bsky.social at @uniheidelberg.bsky.social @bzh-hd.bsky.social . A great team effort! 🙌
April 21, 2025 at 11:59 PM
This work is the result of a shared @dfg.de grant between @walter-nickel-bzh.bsky.social and me, carried out by our joint PhD student @manpreet0700.bsky.social at @uniheidelberg.bsky.social @bzh-hd.bsky.social . A great team effort! 🙌
FGF2 secretion depends on PI(4,5)P₂ asymmetry across the plasma membrane. Disrupting this asymmetry with exogenous PI(4,5)P₂ blocks FGF2 translocation—an effect that reverses as cells restore asymmetry over time
April 21, 2025 at 11:59 PM
FGF2 secretion depends on PI(4,5)P₂ asymmetry across the plasma membrane. Disrupting this asymmetry with exogenous PI(4,5)P₂ blocks FGF2 translocation—an effect that reverses as cells restore asymmetry over time
To quantify FGF2 translocation kinetics, we developed a new single-cell confocal assay. Following doxycycline induction, we removed surface FGF2-GFP with heparin wash and tracked its rapid reappearance—capturing a fresh wave of secretion at different incubation times
April 21, 2025 at 11:59 PM
To quantify FGF2 translocation kinetics, we developed a new single-cell confocal assay. Following doxycycline induction, we removed surface FGF2-GFP with heparin wash and tracked its rapid reappearance—capturing a fresh wave of secretion at different incubation times
If our hypothesis holds, disrupting PI(4,5)P₂ asymmetry in cells should slow FGF2 translocation. To test this, @manpreet0700.bsky.social introduced PI(4,5)P₂ micelles in the media—enriching the outer leaflet and disturbing the natural asymmetry. And yes, it made a difference.
April 21, 2025 at 11:59 PM
If our hypothesis holds, disrupting PI(4,5)P₂ asymmetry in cells should slow FGF2 translocation. To test this, @manpreet0700.bsky.social introduced PI(4,5)P₂ micelles in the media—enriching the outer leaflet and disturbing the natural asymmetry. And yes, it made a difference.
Cells vs. reconstitution systems: Asymmetric PI(4,5)P₂ distribution in living cells accelerates FGF2 translocation—something symmetric GUVs can’t replicate. But when we enzymatically introduced asymmetry into GUVs, the magic finally happened 🪄🎩🐇
April 21, 2025 at 11:59 PM
Cells vs. reconstitution systems: Asymmetric PI(4,5)P₂ distribution in living cells accelerates FGF2 translocation—something symmetric GUVs can’t replicate. But when we enzymatically introduced asymmetry into GUVs, the magic finally happened 🪄🎩🐇
FGF2 translocates in 200ms in cells—but takes ~1hr in reconstitution systems. What drives this rapid kinetics in cells? 🏃♂️⏩⏩
Many auxiliary factors are missing in reconstituted systems. We pinpoint transbilayer PI(4,5)P₂ asymmetry as a key contributor to efficient membrane translocation.
Many auxiliary factors are missing in reconstituted systems. We pinpoint transbilayer PI(4,5)P₂ asymmetry as a key contributor to efficient membrane translocation.
April 21, 2025 at 11:59 PM
FGF2 translocates in 200ms in cells—but takes ~1hr in reconstitution systems. What drives this rapid kinetics in cells? 🏃♂️⏩⏩
Many auxiliary factors are missing in reconstituted systems. We pinpoint transbilayer PI(4,5)P₂ asymmetry as a key contributor to efficient membrane translocation.
Many auxiliary factors are missing in reconstituted systems. We pinpoint transbilayer PI(4,5)P₂ asymmetry as a key contributor to efficient membrane translocation.