Congratulations on the very nice work!

Wooops, Thank you @laurakwhite.bsky.social for poking me :) Indeed we had forgot to make it public - now fixed and should be publicly available, sorry for that!

Btw - i’d strongly recommend to run NanoConsensus through the MasterOfPores Nextflow workflow - which was already public :)

We would also thank the community for their interest in our work, which encouraged us to put the time and effort in upgrading Nano3P-seq to R10 chemistry. If you use the pipeline and/or code, please let us know how it goes - feedback very welcome! :)

6) This was a great team effort of @oguzhanbegik.bsky.social and #LeszekPryszcz.

5) PolyTailor is a new tool that can predict tail length and content without relying on the annotation of polyA sites on Nano3P-seq data. It is a user-friendly and fast, code is already available to use! github.com/novoalab/pol...

4) Deprecation of the R9 chemistry also led to the deprecation of basecalled fast5 files, which were crucial for Nano3P-seq tail length prediction analysis. So we released a new in-house analysis tool called PolyTailor!

3) We replaced the deprecated ONT kits DCS109&NBD104 with NBD114.24 barcoding kit. This kit is compatible with the new R10 chemistry and allows barcoding up to 24 libraries.

2) Template-switching oligos used for Nano3P-seq had a sequence context that was decreasing the tail prediction efficiency (due to TTC sequence at the adapter sequence end). We now changed our oligo sequence to improve tail prediction efficiency.

So, what did we change? 1) TGIRT, a group II intron RT we used for template-switching reaction of Nano3P-seq library preparation had been depracated. Fortunately, @NEBiolabs had recently released Induro, a highly processive group II intron RT, so we adapted our method to use this enzyme