Erh-Min Lai-IPMB
@erhminlai.bsky.social
Research Fellow and Professor at the Institute of Plant and Microbial Biology, Academia Sinica, Taipei, #Taiwan. #Agrobacterium, #MPMI., #T4SS, #T6SS. https://ipmb.sinica.edu.tw/en/people/ipmb_researchers/lai-erh-min
Reposted by Erh-Min Lai-IPMB
(🧵 8/8) By integrating floral-stage selection, immune evasion, and Agrobacterium engineering, the team established a practical platform to boost plant genetic engineering efficiency in Arabidopsis.
December 2, 2025 at 8:43 PM
(🧵 8/8) By integrating floral-stage selection, immune evasion, and Agrobacterium engineering, the team established a practical platform to boost plant genetic engineering efficiency in Arabidopsis.
Reposted by Erh-Min Lai-IPMB
(🧵 6/8) The team achieved several milestone discoveries for improving Agrobacterium-mediated transient expression, stable transformation, and genome-editing efficiencies in plants.
December 2, 2025 at 8:43 PM
(🧵 6/8) The team achieved several milestone discoveries for improving Agrobacterium-mediated transient expression, stable transformation, and genome-editing efficiencies in plants.
Reposted by Erh-Min Lai-IPMB
(🧵 5/8) ... work to advance solutions to overcoming barriers in Agrobacterium-mediated transformation (AMT) through an Institutional Grand Challenge Project.
December 2, 2025 at 8:43 PM
(🧵 5/8) ... work to advance solutions to overcoming barriers in Agrobacterium-mediated transformation (AMT) through an Institutional Grand Challenge Project.
Reposted by Erh-Min Lai-IPMB
🌱 The authors behind the paper 🌱
(🧵 4/8) A research team at the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan, led by Project Director Erh-Min Lai, with co-PIs Chih-Horng Kuo and Chih-Hang Wu...
(🧵 4/8) A research team at the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan, led by Project Director Erh-Min Lai, with co-PIs Chih-Horng Kuo and Chih-Hang Wu...
December 2, 2025 at 8:43 PM
🌱 The authors behind the paper 🌱
(🧵 4/8) A research team at the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan, led by Project Director Erh-Min Lai, with co-PIs Chih-Horng Kuo and Chih-Hang Wu...
(🧵 4/8) A research team at the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan, led by Project Director Erh-Min Lai, with co-PIs Chih-Horng Kuo and Chih-Hang Wu...
Reposted by Erh-Min Lai-IPMB
(🧵 3/8) These improvements significantly enhanced transient gene expression and CRISPR/Cas-mediated genome editing, enabling efficient recovery of edited plants without selection. This work offers a practical, high-efficiency platform to advance plant genome engineering.
December 2, 2025 at 8:43 PM
(🧵 3/8) These improvements significantly enhanced transient gene expression and CRISPR/Cas-mediated genome editing, enabling efficient recovery of edited plants without selection. This work offers a practical, high-efficiency platform to advance plant genome engineering.
Reposted by Erh-Min Lai-IPMB
(🧵 2/8) In this Methods paper Liu et al. explain how they developed an optimized Agrobacterium-mediated floral transformation in Arabidopsis thaliana by identifying the most receptive floral stage and engineering agrobacterial strains to evade plant immunity.
December 2, 2025 at 8:43 PM
(🧵 2/8) In this Methods paper Liu et al. explain how they developed an optimized Agrobacterium-mediated floral transformation in Arabidopsis thaliana by identifying the most receptive floral stage and engineering agrobacterial strains to evade plant immunity.
Reposted by Erh-Min Lai-IPMB
(🧵 7/8) This study was primarily conducted by co-first authors Mao-Sen Liu (Project Research Scientist) and Teng-Kuei Huang (Postdoctoral Fellow), with support from Technicians Yi-Chieh Wang and Si-Chong Wang.
December 2, 2025 at 8:43 PM
(🧵 7/8) This study was primarily conducted by co-first authors Mao-Sen Liu (Project Research Scientist) and Teng-Kuei Huang (Postdoctoral Fellow), with support from Technicians Yi-Chieh Wang and Si-Chong Wang.
Reposted by Erh-Min Lai-IPMB
✨ Paper spotlight ✨
(🧵 1/8) Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis
nph.onlinelibrary.wiley.com/doi/10.1111/...
(🧵 1/8) Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis
nph.onlinelibrary.wiley.com/doi/10.1111/...
December 2, 2025 at 8:43 PM
✨ Paper spotlight ✨
(🧵 1/8) Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis
nph.onlinelibrary.wiley.com/doi/10.1111/...
(🧵 1/8) Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis
nph.onlinelibrary.wiley.com/doi/10.1111/...
Reposted by Erh-Min Lai-IPMB
Now we have Chih-hang Wu @IPMBSinica Taiwan 🇹🇼 at #PPATH2025 — Fast and Furious: What happens after helper NLR immune receptors are activated to cause hypersensitive cell death.
See paper doi.org/10.1101/2025...
See paper doi.org/10.1101/2025...
September 11, 2025 at 1:29 PM
Now we have Chih-hang Wu @IPMBSinica Taiwan 🇹🇼 at #PPATH2025 — Fast and Furious: What happens after helper NLR immune receptors are activated to cause hypersensitive cell death.
See paper doi.org/10.1101/2025...
See paper doi.org/10.1101/2025...
Reposted by Erh-Min Lai-IPMB
Connections are in action in Cologne! At #2025ISMPMI, the poster sessions aren’t just about groundbreaking research—they’re about meeting peers, collaboration, and sharing ideas that move plant-microbe science forward.
Full poster hours here: bit.ly/44LqCvc
#plantscience #networking
Full poster hours here: bit.ly/44LqCvc
#plantscience #networking
July 14, 2025 at 4:25 PM
Connections are in action in Cologne! At #2025ISMPMI, the poster sessions aren’t just about groundbreaking research—they’re about meeting peers, collaboration, and sharing ideas that move plant-microbe science forward.
Full poster hours here: bit.ly/44LqCvc
#plantscience #networking
Full poster hours here: bit.ly/44LqCvc
#plantscience #networking
Reposted by Erh-Min Lai-IPMB
It was an amazing sciences day at #2025ISMPMI!
Right after the “Population, biology, ecology and genomics” session, I had the opportunity to have lunch and discuss about this topic with the co-chair @honour.bsky.social
Right after the “Population, biology, ecology and genomics” session, I had the opportunity to have lunch and discuss about this topic with the co-chair @honour.bsky.social
July 14, 2025 at 9:19 PM
It was an amazing sciences day at #2025ISMPMI!
Right after the “Population, biology, ecology and genomics” session, I had the opportunity to have lunch and discuss about this topic with the co-chair @honour.bsky.social
Right after the “Population, biology, ecology and genomics” session, I had the opportunity to have lunch and discuss about this topic with the co-chair @honour.bsky.social
Great pleasure hosting @fuquaclay.bsky.social visiting IPMB, Academia Sinica. Enjoy scientific discussion, casual chatting over delicious meals and outing. #Agrobacterium #Taiwan
June 7, 2025 at 1:51 AM
Great pleasure hosting @fuquaclay.bsky.social visiting IPMB, Academia Sinica. Enjoy scientific discussion, casual chatting over delicious meals and outing. #Agrobacterium #Taiwan
Reposted by Erh-Min Lai-IPMB
Check out the ASM 10th Conference on Biofilms Bluesky page @asmbiofilms2025.bsky.social
December 18, 2024 at 12:33 PM
Check out the ASM 10th Conference on Biofilms Bluesky page @asmbiofilms2025.bsky.social
Reposted by Erh-Min Lai-IPMB
Happy to share a new publication on a collaboration with Xindan Wang and @fuquaclay.bsky.social labs. We identified and characterized naturally occurring Agrobacterium strains with fused chromosomes. Did mobile genetic elements play a role in fusion? journals.asm.org/doi/10.1128/...
Linear dicentric chromosomes in bacterial natural isolates reveal common constraints for replicon fusion | mBio
Most bacterial genomes are monopartite with a single, circular chromosome. However,
some species, like Agrobacterium tumefaciens, carry multiple chromosomes. Emergence of multipartite genomes is often...
journals.asm.org
May 20, 2025 at 2:37 PM
Happy to share a new publication on a collaboration with Xindan Wang and @fuquaclay.bsky.social labs. We identified and characterized naturally occurring Agrobacterium strains with fused chromosomes. Did mobile genetic elements play a role in fusion? journals.asm.org/doi/10.1128/...
Reposted by Erh-Min Lai-IPMB
Interesting MS on Agrobacterium cooperation and antagonism as well as development of dual binary system that mitigates plant immune antagonism. www.nature.com/articles/s41...
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Quantitative dissection of Agrobacterium T-DNA expression in single plant cells reveals density-dependent synergy and antagonism - Nature Plants
Alamos et al. show that Agrobacterium engages in density-dependent interactions that dictate transformation efficiency and transgene expression. These findings enable a quantitative model to improve m...
www.nature.com
May 16, 2025 at 6:19 PM
Interesting MS on Agrobacterium cooperation and antagonism as well as development of dual binary system that mitigates plant immune antagonism. www.nature.com/articles/s41...
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Happy to write this News & Views highlighting a groundbreaking study on #Agrobacterium. By combining mathematical modelling with quantitative live-cell imaging, the study uncovered a density-dependent synergistic and antagonistic effect on #AMT
New OA Article: "Quantitative dissection of Agrobacterium T-DNA expression in single plant cells reveals density-dependent synergy and antagonism" rdcu.be/elJ5z
News & Views: "Cooperation and antagonism in Agrobacterium-mediated transformation" rdcu.be/elJ5F
News & Views: "Cooperation and antagonism in Agrobacterium-mediated transformation" rdcu.be/elJ5F
May 13, 2025 at 2:22 PM
Happy to write this News & Views highlighting a groundbreaking study on #Agrobacterium. By combining mathematical modelling with quantitative live-cell imaging, the study uncovered a density-dependent synergistic and antagonistic effect on #AMT
Reposted by Erh-Min Lai-IPMB
Our Research Briefing on the tandem kinase proteins paper is online now!
Curious about the story behind our TKP paper?
📄 Research Briefing: www.nature.com/articles/s41...
📄 Research article: www.nature.com/articles/s41...
Curious about the story behind our TKP paper?
📄 Research Briefing: www.nature.com/articles/s41...
📄 Research article: www.nature.com/articles/s41...
A plant dual-kinase protein traps a pathogen effector to trigger immunity - Nature Genetics
Tandem kinase proteins (TKPs) are key regulators of plant immunity. We found that RWT4, a wheat TKP, confers resistance to the fungal pathogen Magnaporthe oryzae by directly recognizing the effector A...
www.nature.com
April 25, 2025 at 6:30 PM
Our Research Briefing on the tandem kinase proteins paper is online now!
Curious about the story behind our TKP paper?
📄 Research Briefing: www.nature.com/articles/s41...
📄 Research article: www.nature.com/articles/s41...
Curious about the story behind our TKP paper?
📄 Research Briefing: www.nature.com/articles/s41...
📄 Research article: www.nature.com/articles/s41...
Reposted by Erh-Min Lai-IPMB
👀 EFR-mediated PTI restricts transient transformation and genome editing, but not T-DNA integration.
This study combines floral stage targeting 🌷, immune evasion 🛡️, and Agrobacterium engineering 🔧 to boost plant genome editing efficiency.
#Agrobacterium
#GenomeEditing
www.biorxiv.org/content/10.1...
This study combines floral stage targeting 🌷, immune evasion 🛡️, and Agrobacterium engineering 🔧 to boost plant genome editing efficiency.
#Agrobacterium
#GenomeEditing
www.biorxiv.org/content/10.1...
Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis
Agrobacterium-mediated transformation via floral inoculation (AMT-FI) enables genetic engineering without tissue culture. It is widely used in the model plant Arabidopsis thaliana, yet its efficiency ...
www.biorxiv.org
May 9, 2025 at 8:24 PM
👀 EFR-mediated PTI restricts transient transformation and genome editing, but not T-DNA integration.
This study combines floral stage targeting 🌷, immune evasion 🛡️, and Agrobacterium engineering 🔧 to boost plant genome editing efficiency.
#Agrobacterium
#GenomeEditing
www.biorxiv.org/content/10.1...
This study combines floral stage targeting 🌷, immune evasion 🛡️, and Agrobacterium engineering 🔧 to boost plant genome editing efficiency.
#Agrobacterium
#GenomeEditing
www.biorxiv.org/content/10.1...
Reposted by Erh-Min Lai-IPMB
Nice preprint from Erh-Min Lai's lab. "By integrating floral stage selection, immune evasion, and Agrobacterium engineering, this study provides a practical and versatile platform to advance plant genome engineering." vhttps://www.biorxiv.org/content/10.1101/2025.05.07.652770v1
Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis
Agrobacterium-mediated transformation via floral inoculation (AMT-FI) enables genetic engineering without tissue culture. It is widely used in the model plant Arabidopsis thaliana, yet its efficiency ...
www.biorxiv.org
May 9, 2025 at 5:30 PM
Nice preprint from Erh-Min Lai's lab. "By integrating floral stage selection, immune evasion, and Agrobacterium engineering, this study provides a practical and versatile platform to advance plant genome engineering." vhttps://www.biorxiv.org/content/10.1101/2025.05.07.652770v1
Reposted by Erh-Min Lai-IPMB
Tweeps, please spread the word. We're still looking at recruiting a structural biologist (any level) to study plant-pathogen protein complexes especially in the context of coevolution. Direct inquiries welcome.
Please include a cover letter.
Applicant from US welcome? medium.com/@kamounlab/2...
Please include a cover letter.
Applicant from US welcome? medium.com/@kamounlab/2...
May 9, 2025 at 1:13 PM
Tweeps, please spread the word. We're still looking at recruiting a structural biologist (any level) to study plant-pathogen protein complexes especially in the context of coevolution. Direct inquiries welcome.
Please include a cover letter.
Applicant from US welcome? medium.com/@kamounlab/2...
Please include a cover letter.
Applicant from US welcome? medium.com/@kamounlab/2...