!

And they don’t have a single archaeon. I am glad that a few years back Colleen Cavanaugh gifted me a methanogen plushie for my son. You cannot get them anymore.

'PI not included'

Die Fans eines 2000 Jahre alten Fantasy Romans, dessen viele Autoren Geschichten aus der spaeten Bronze-Zeit neu interpretierten, versuchen einen anderen Fan des gleichen Fantasy Romans davon zu ueberzeugen das er ihren Superhelden falsch versteht.

Ist das up-to-date? Also inklusive abgerissenem Ostfluegel?

It is crazy that we need to write a long article for adults to tell then something that my 4 year old knowns.

That’s at least my hope

Joelie Van Beek was supported by a ERFP by the National Science Foundation.

journals.asm.org/doi/epdf/10....

Work funded by the Department of Energy and performed in collaboration with the @jgi.doe.gov Shared first authors are my grad student Joelie Van Beek and undergraduate researcher Grayson Robles.

We hope that by making these CloneFISH cultures available to the scientific community more laboratories will incorporate fluorescence in situ hybridization experiments of archaea into their technical portfolio

The 30 clones represent all four archaeal super-phyla (Asgard, DPANN, Euryarchaeota, and Thermoproteota), but in contrast to the archaea they represent, can easily be grown in LB medium without the need of specialized equipment or techniques

each carrying a plasmid for the heterologous expression of a 16S rRNA gene from one of 30 high-rank taxa of archaea. From these, 17 clones represent lineages that are currently uncultured and 13 represent lineages of which at least one member has been obtained in (pure or enrichment) culture.

Lack of access to pure cultures and other positive controls limit the wide-spread use of fluorescence in situ hybridization to visualize and quantify cells in complex environmental and human microbiome samples. My lab has produced a collection of 30 Escherichia coli cultures (CloneFISH cultures),

Contact me if you want a glycerol stock and/or PFA-fixed cells that already express the archaeal 16S rRNA! Or get the plasmid from AddGene and clone it yourself. Here's the complete list of archaeal lineages available via CloneFISH. #microsky 🦠