Thanks, will try it ! We use cells for unstained/autofluorescence and viability, for the other markers we use beads

Thanks a lot for the informative thread! Looks very exciting

Are single stained cellular controls necessary or only better ? We are using rare human samples so it's difficult to cells for single color controls, can we still hope to see improvements with AutoSpectral unmixing ?

6. found just enough strength to relay dread while being funny

That is no tiny victory.

I hope you find support until you are strong enough to support those who need it !