High-quality genome assemblies depend not only on sequencing depth and read length, but also on careful attention to upstream sample handling and library preparation. This webinar provides a practical, end-to-end overview of working with the Dovetail LinkPrep Kit for genome assembly applications, with a focus on generating robust proximity-ligation data that maximizes scaffolding performance. We will begin by discussing best practices for sample collection, preservation, and transportation to ensure the integrity of high–molecular weight DNA across a range of organisms and sample types. The session will then move into a detailed walkthrough of the Dovetail LinkPrep workflow, highlighting key tips and common pitfalls during chromatin fixation, library construction, and hands-on execution. Guidance on library quality control metrics and interpretation will be provided, along with recommended sequencing parameters to support efficient and accurate scaffolding. This webinar is designed for researchers and core facilities seeking practical guidance to optimize their genome assembly workflows using Dovetail LinkPrep, from sample intake through to final scaffolded assemblies.

High-quality genome assemblies depend not only on sequencing depth and read length, but also on careful attention to upstream sample handling and library preparation. This webinar provides a practical, end-to-end overview of working with the Dovetail LinkPrep Kit for genome assembly applications, with a focus on generating robust proximity-ligation data that maximizes scaffolding performance. We will begin by discussing best practices for sample collection, preservation, and transportation to ensure the integrity of high–molecular weight DNA across a range of organisms and sample types. The session will then move into a detailed walkthrough of the Dovetail LinkPrep workflow, highlighting key tips and common pitfalls during chromatin fixation, library construction, and hands-on execution. Guidance on library quality control metrics and interpretation will be provided, along with recommended sequencing parameters to support efficient and accurate scaffolding. This webinar is designed for researchers and core facilities seeking practical guidance to optimize their genome assembly workflows using Dovetail LinkPrep, from sample intake through to final scaffolded assemblies.