Thanks Matt, didn't realize you were in the e-audience!

I'm certain there are Etsy shops that can do this!

This matches a bunch of data we generated showing that knocking down or inhibiting acyltransferases does not impact global protein lactylation levels. Read the paper (open access!) to get more detail about why we think this pathway is so interesting and physiologically relevant! And more to come!

Cultured cells contain lactylated proteins but adding HDAC inhibitors reduces this. Importantly, this inhibition is independent of intracellular lactate or lactyl-CoA concentrations (thank you @mzspectrum.bsky.social for helping with this!). We uncoupled Kla from lactyl-CoA!

We established biochemically that HDACs can indeed catalyze the lactylation of proteins. A lysine protection assay with fluorescent peptide also revealed the reaction can occur well within physiological lactate levels, making this basally more likely than the proposed lactyl-CoA mechanism

This is a follow up to our prior work where we published our discovery about HDACs beta-hydroxybutyrylating proteins

www.nature.com/articles/s41...