Thanks, Jo! 🤩

We hypothesize the expansions of:
1) HSP20 = good at surviving heat 🔥

2) GST = good at dealing with ROS generated during stress & in response to metabolism of PyOm ⚠️

3) ACD = good at metabolising PyOm 🍽

Taken together, R. undulata survives fire and then thrives in post-fire environments!

3 protein family expansions were discovered in R. undulata. The HSP20 expansion was present in the other fire-associates, but the 2 others were unique to R. undulata! This included GST and ACD enzymes. See below! 🔽

Aww, that's bad timing! But good luck in the lab, I hope it goes well ✨️🧪

🧪🧬 We acknowledge support from FABI, UCPH, The Novo Nordisk Foundation, and the NRF! And I thank my co-authors for their contributions to this manuscript 🍄🤩

🧪🧬 We also provide a schematic and lots of in text details on how you can optimize this protocol for use in your own species! 🧬🧪

🧪🧬 Protocol 5 is for Sclerotinia sclerotiorum- the fungus with a host range of more than 400 plant species! 🌾🍀🌿🌳 This protocol uses immature mycelium as a starting point

🧪🧬 Protocol 4 is for Ophiostoma novo-ulmi, the Dutch elm disease pathogen 🌲🍄 This protocol uses a DTT-EDTA pre-treatment and uses conidia as the starting material ⚗️

🧪🧬 Protocol 3 is for Metarhizium species- fungi that infect and cause death in insects, including various agricultural pests 🦗 The protocol uses mature mycelium as a starting point.

🧪🧬 Protocol 2 is for Fusarium circinatum- the notorious pine pathogen that causes devastation in natural and managed forests 🌲 This protocol describes extraction from a mixture of mycelium and conidia

🧪🧬 Protocol 1 is for Ceratocystis species and describes the extraction of protoplasts from mycelium, using a density gradient to purify the protoplasts 🍄🌲

We present 5 protocols, each optimized in a different fungal genus, for species important in agriculture 🌾 & forestry 🌲

We use an enzyme from the wine industry, #Extralyse, to overcome the reliance on cost-prohibitive lab-grade enzymes whose availability is unreliable 🥲🧪🧬

Wednesday was the first sampling day and... my root and locust media was contaminated by this bacteria left 🧫

But after some tears and a restless night, I know what went wrong and how to fix it 💪

Hopefully with the added experience, setting up Round 2 won't take as long! 🤞