DjuranovicLab at Brown University RNA Center
@djuranoviclab.bsky.social
RNA, Ribosome, miRNA, mRNA, translation, translational control, sometimes splicing
On the way back from one week of great science, technology and therapeutic potentials of RNA in organization of Immagina Biotechnology S.r.l. and Massimiliano Clamer. Thanks for the great meeting to Max and the Immagina team. Looking forward to future collaborations.
#RNA #tRNA #ribosome
#RNA #tRNA #ribosome
October 24, 2025 at 7:29 AM
Four days of science at
@events.embl.org
in Heidelberg for "Protein Synthesis and Translational Control" meeting.
@events.embl.org
in Heidelberg for "Protein Synthesis and Translational Control" meeting.
September 3, 2025 at 5:15 PM
Four days of science at
@events.embl.org
in Heidelberg for "Protein Synthesis and Translational Control" meeting.
@events.embl.org
in Heidelberg for "Protein Synthesis and Translational Control" meeting.
First RAPPL ribosome isolation at the new lab space at Brown University. Purification from a small culture of parasitic Eukaryote. Soon out...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
July 15, 2025 at 5:36 PM
First RAPPL ribosome isolation at the new lab space at Brown University. Purification from a small culture of parasitic Eukaryote. Soon out...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Last month @uspto.bsky.social
issued yet another patent to Slavica Pavlovic Djuranovic, Jessey Erath and @djuranoviclab.bsky.social for use of polybasic peptide or non-peptide moieties against malaria parasites. More soon...
issued yet another patent to Slavica Pavlovic Djuranovic, Jessey Erath and @djuranoviclab.bsky.social for use of polybasic peptide or non-peptide moieties against malaria parasites. More soon...
May 8, 2025 at 8:19 PM
Last month @uspto.bsky.social
issued yet another patent to Slavica Pavlovic Djuranovic, Jessey Erath and @djuranoviclab.bsky.social for use of polybasic peptide or non-peptide moieties against malaria parasites. More soon...
issued yet another patent to Slavica Pavlovic Djuranovic, Jessey Erath and @djuranoviclab.bsky.social for use of polybasic peptide or non-peptide moieties against malaria parasites. More soon...
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (6/6). Archaea #1
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
April 28, 2025 at 3:15 PM
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (6/6). Archaea #1
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (5/6). Eukaryotic #3
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
April 28, 2025 at 3:13 PM
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (5/6). Eukaryotic #3
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (4/6). Eukaryotic #2
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
April 28, 2025 at 3:11 PM
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (4/6). Eukaryotic #2
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (3/6). Eukaryotic #1
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
April 28, 2025 at 3:09 PM
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (3/6). Eukaryotic #1
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (1/6). Bacterial #2
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
April 28, 2025 at 3:08 PM
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (1/6). Bacterial #2
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (1/6). Bacterial #1
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
April 28, 2025 at 3:06 PM
Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (1/6). Bacterial #1
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Ready for the next RNA session in @asbmb.bsky.social meeting. We start soon...
April 14, 2025 at 6:35 PM
Ready for the next RNA session in @asbmb.bsky.social meeting. We start soon...
Ribosome data collected from a new and precious sample of 10,000,000 cells from a strange organism. All done using RAPPL, more soon...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
March 28, 2025 at 9:20 PM
Ribosome data collected from a new and precious sample of 10,000,000 cells from a strange organism. All done using RAPPL, more soon...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
This is how squirrel's nest looked like in our car many years ago.
February 23, 2025 at 3:20 AM
This is how squirrel's nest looked like in our car many years ago.
One of the best single step purification preparations of protozoan ribosomes I've ever seen. Combination of RAPPL (biorxiv.org/content/10.1...) and a new technique. More at rna.ucsc.edu/rnacenter/ri...
February 21, 2025 at 4:34 PM
One of the best single step purification preparations of protozoan ribosomes I've ever seen. Combination of RAPPL (biorxiv.org/content/10.1...) and a new technique. More at rna.ucsc.edu/rnacenter/ri...
Ok after @cryoclem.bsky.social we also tried Archaeal ribosomes (and some proteasomes) with RAPPL (www.biorxiv.org/content/10.1...) as well as some specific translation cycle complexes with new method.
February 14, 2025 at 2:06 AM
Ok after @cryoclem.bsky.social we also tried Archaeal ribosomes (and some proteasomes) with RAPPL (www.biorxiv.org/content/10.1...) as well as some specific translation cycle complexes with new method.
After RAPPL (www.biorxiv.org/content/10.1...) we found another way to get ribosomes and other macromolecular complexes easier to the cryoEM grids. This time besides ribosomes p97 complexes were purified (back to the PhD times). Stayed tuned...
February 12, 2025 at 1:10 AM
After RAPPL (www.biorxiv.org/content/10.1...) we found another way to get ribosomes and other macromolecular complexes easier to the cryoEM grids. This time besides ribosomes p97 complexes were purified (back to the PhD times). Stayed tuned...
As I noted more to come from RAPPL (www.biorxiv.org/content/10.1...)
January 31, 2025 at 5:49 PM
As I noted more to come from RAPPL (www.biorxiv.org/content/10.1...)
In addition to today's posted work by @cryoclem.bsky.social on Archaeal ribosomes we further expanded RAPPL (www.biorxiv.org/content/10.1...) on multiple other complexes that work with ribosomes, subunits or even off the ribosome. Soon more...
January 30, 2025 at 4:06 PM
In addition to today's posted work by @cryoclem.bsky.social on Archaeal ribosomes we further expanded RAPPL (www.biorxiv.org/content/10.1...) on multiple other complexes that work with ribosomes, subunits or even off the ribosome. Soon more...
Some translating but stalled bacterial ribosomes. Tandem purification using RAPPL. Ready for cryoEM...
January 18, 2025 at 12:19 PM
Some translating but stalled bacterial ribosomes. Tandem purification using RAPPL. Ready for cryoEM...
We are down to 2.32A and 2.61A for these specific ribosome complexes using tandem purification using RAPPL and tagged protein enrichment. In this case it was and hour and 45 minutes prep and 20-50 ml bacterial culture.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
January 12, 2025 at 10:56 PM
We are down to 2.32A and 2.61A for these specific ribosome complexes using tandem purification using RAPPL and tagged protein enrichment. In this case it was and hour and 45 minutes prep and 20-50 ml bacterial culture.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
When your cryoEM run and data collection is not finished but still shows what you were looking for. Another one from 2ml culture.
December 20, 2024 at 6:00 PM
When your cryoEM run and data collection is not finished but still shows what you were looking for. Another one from 2ml culture.
More ribosome structures solved by RAPPL (collaboration with Pavlovic Djuranovic and Hashem Labs). This one is bacterial from 2ml culture and using method described in our preprint.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
December 16, 2024 at 3:34 AM
More ribosome structures solved by RAPPL (collaboration with Pavlovic Djuranovic and Hashem Labs). This one is bacterial from 2ml culture and using method described in our preprint.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Congratulations to Dr.Courtney Jungers for successful PhD defense. Another PhD from
@djuranoviclab.bsky.social
@djuranoviclab.bsky.social
December 11, 2024 at 1:44 AM
Congratulations to Dr.Courtney Jungers for successful PhD defense. Another PhD from
@djuranoviclab.bsky.social
@djuranoviclab.bsky.social
Bye Tokyo and Japan till next meeting. This was such a great experience. Greetings to all great scientists that we met over the last three weeks in Japan.
December 5, 2024 at 6:24 AM
Bye Tokyo and Japan till next meeting. This was such a great experience. Greetings to all great scientists that we met over the last three weeks in Japan.
Second day of Ribosome meeting at the University of Tokyo. With Slavica Pavlovic Djuranovic.
December 3, 2024 at 12:27 AM
Second day of Ribosome meeting at the University of Tokyo. With Slavica Pavlovic Djuranovic.