André Dias
@dias-andre.bsky.social
Developmental biologist @AMAlab @upf. Playing with cells & genes to understand the role of signalling during mammalian gastrulation. PhD @IGCiencia
Pinned
André Dias
@dias-andre.bsky.social
· Jan 13
A temporal coordination between Nodal and Wnt signalling governs the emergence of the mammalian body plan
Nodal and Wnt signalling play an important role in the emergence of the mammalian body plan, primarily by orchestrating gastrulation and primitive streak formation. While the literature suggests that ...
www.biorxiv.org
www.biorxiv.org/content/10.1...
Here we explore the role of #Wnt and #Nodal signalling during mammalian gastrulation using #gastruloids. Building on the work of many labs, we propose that antagonistic signalling-dependent #modularity in the PS establishes the early mammalian body plan.
Here we explore the role of #Wnt and #Nodal signalling during mammalian gastrulation using #gastruloids. Building on the work of many labs, we propose that antagonistic signalling-dependent #modularity in the PS establishes the early mammalian body plan.
Reposted by André Dias
The power of the media: core culture conditions direct gastruloid development
This Research Highlight showcases the work from Tina Balayo, André Dias @dias-andre.bsky.social, David Turner @gastruloids.bsky.social and colleagues:
journals.biologists.com/dev/article/...
This Research Highlight showcases the work from Tina Balayo, André Dias @dias-andre.bsky.social, David Turner @gastruloids.bsky.social and colleagues:
journals.biologists.com/dev/article/...
![NDiff227 gastruloids display a developmental delay in comparison to HM-N2B27 gastruloids. (A) Quantitative analysis of the roundness (perimeter2/4π×area) of gastruloids cultured in either HM-N2B27 or NDiff227 showed that HM-N2B27 gastruloids are significantly less ‘Round’ than their NDiff227 counterparts at 96 h [unpaired two-tailed t-test; t(4)=−4.9541, P=0.008]. Each datapoint is the average roundness of an independent replicate experiment containing between ∼40 and 90 individual gastruloids (see Fig. S4B). (B) The frequency of morphologies from the data in A, with examples of gastruloids that would fall in each category (bottom; the left 'Protrusions' image is also shown in Fig. S4). (C) Light-sheet representative images (maximum projection images) highlighting the expression patterns of Cdh1 (magenta) and Cdh2 (cyan) between HM-N2B27 gastruloids at 96 h and NDiff227 gastruloids at 90 h and 96 h. Gastruloids developed in HM-N2B27 have upregulated Cdh2 by 90 h, unlike NDiff227 gastruloids, which show enhanced Cdh2 only at 96 h. Scale bars: 100 μm. (Ci) Qualitative analysis of the average expression pattern of Cdh2 (N-cadherin). The expression patterns were grouped into two categories (phenotypes I and II) and represent the average of three independent replicate experiments, each containing at least seven individual gastruloids per condition. Examples of the two categories are shown below. The leftmost example for ‘Phenotype I’ is shown in the middle row of 90 h HM-N2B27 (Cdh2) in C. For the box and whisker plot, the central line in the box is the median, the lower edge of the box is the first quartile, the upper edge of the box is the third quartile, and the box height is the IQR. The lower and upper whiskers indicate the smallest datapoint within 1.5xIQR below Q1 or above Q3, respectively.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:btitwyhiegkb6f62oguxt6m6/bafkreiakq7wvgnsnajj2g3bpn5k5p4yu7vznwibobhgzu2o5ttsmjq5ylq@jpeg)
November 26, 2025 at 4:13 PM
The power of the media: core culture conditions direct gastruloid development
This Research Highlight showcases the work from Tina Balayo, André Dias @dias-andre.bsky.social, David Turner @gastruloids.bsky.social and colleagues:
journals.biologists.com/dev/article/...
This Research Highlight showcases the work from Tina Balayo, André Dias @dias-andre.bsky.social, David Turner @gastruloids.bsky.social and colleagues:
journals.biologists.com/dev/article/...
Reposted by André Dias
To find out more about this work and the people behind it, we spoke to first author Tina Balayo, and to co-corresponding authors André Dias @dias-andre.bsky.social and David Turner @gastruloids.bsky.social:
journals.biologists.com/dev/article/...
journals.biologists.com/dev/article/...
November 26, 2025 at 4:13 PM
To find out more about this work and the people behind it, we spoke to first author Tina Balayo, and to co-corresponding authors André Dias @dias-andre.bsky.social and David Turner @gastruloids.bsky.social:
journals.biologists.com/dev/article/...
journals.biologists.com/dev/article/...
We would like to thank the reviewers for their inputs, the editor, and @dev-journal.bsky.social for the interview journals.biologists.com/dev/article/... and the highlight journals.biologists.com/dev/article/...
November 26, 2025 at 9:01 AM
We would like to thank the reviewers for their inputs, the editor, and @dev-journal.bsky.social for the interview journals.biologists.com/dev/article/... and the highlight journals.biologists.com/dev/article/...
Reposted by André Dias
I do like the @dev-journal.bsky.social "Research Report" format for shortish papers
This one is valuable. "Is it the N2B27?" is the stem cell biologist version of "Is it the magnesium?"
Congrats Tina Balayo @dias-andre.bsky.social David Turner & all authors
journals.biologists.com/dev/article/...
This one is valuable. "Is it the N2B27?" is the stem cell biologist version of "Is it the magnesium?"
Congrats Tina Balayo @dias-andre.bsky.social David Turner & all authors
journals.biologists.com/dev/article/...
N2B27 media formulations influence gastruloid development
Highlighted Article: This work explores the cellular and molecular sensitivity of the mouse gastruloid model system to variations in N2B27 media formulations, highlighting the need for careful media s...
journals.biologists.com
November 25, 2025 at 11:22 AM
I do like the @dev-journal.bsky.social "Research Report" format for shortish papers
This one is valuable. "Is it the N2B27?" is the stem cell biologist version of "Is it the magnesium?"
Congrats Tina Balayo @dias-andre.bsky.social David Turner & all authors
journals.biologists.com/dev/article/...
This one is valuable. "Is it the N2B27?" is the stem cell biologist version of "Is it the magnesium?"
Congrats Tina Balayo @dias-andre.bsky.social David Turner & all authors
journals.biologists.com/dev/article/...
Now out in @dev-journal.bsky.social
Lots of new results! Check it out!!
journals.biologists.com/dev/article/...
Lots of new results! Check it out!!
journals.biologists.com/dev/article/...
November 25, 2025 at 10:37 AM
Now out in @dev-journal.bsky.social
Lots of new results! Check it out!!
journals.biologists.com/dev/article/...
Lots of new results! Check it out!!
journals.biologists.com/dev/article/...
Reposted by André Dias
Happy to present the final version of our Cadherin and gastruloid manuscript, online at @cp-cellreports.bsky.social.
www.cell.com/cell-reports...
Initially in BiorXiv (www.biorxiv.org/content/10.1...) 1/12
www.cell.com/cell-reports...
Initially in BiorXiv (www.biorxiv.org/content/10.1...) 1/12
Cadherins modulate the self-organizing potential of pseudo-embryos
Gastruloids derived from ES cells mimic embryonic patterning through robust self-organization.
Mayran et al. uncover that this competence depends on a cadherin switch regulated
by Snai1-driven E-cadhe...
www.cell.com
November 17, 2025 at 10:37 AM
Happy to present the final version of our Cadherin and gastruloid manuscript, online at @cp-cellreports.bsky.social.
www.cell.com/cell-reports...
Initially in BiorXiv (www.biorxiv.org/content/10.1...) 1/12
www.cell.com/cell-reports...
Initially in BiorXiv (www.biorxiv.org/content/10.1...) 1/12
Reposted by André Dias
🚨JOB ALERT🚨
FRIENDS PLEASE SPREAD and RT
We are building a small expert team within the @bokelab.bsky.social at @crg.eu @prbb.org to investigate fundamental questions in oocyte cell biology, focusing on how proteostasis regulation influences dormancy and fertility.
See below 2 calls👇
FRIENDS PLEASE SPREAD and RT
We are building a small expert team within the @bokelab.bsky.social at @crg.eu @prbb.org to investigate fundamental questions in oocyte cell biology, focusing on how proteostasis regulation influences dormancy and fertility.
See below 2 calls👇
October 2, 2025 at 11:00 AM
🚨JOB ALERT🚨
FRIENDS PLEASE SPREAD and RT
We are building a small expert team within the @bokelab.bsky.social at @crg.eu @prbb.org to investigate fundamental questions in oocyte cell biology, focusing on how proteostasis regulation influences dormancy and fertility.
See below 2 calls👇
FRIENDS PLEASE SPREAD and RT
We are building a small expert team within the @bokelab.bsky.social at @crg.eu @prbb.org to investigate fundamental questions in oocyte cell biology, focusing on how proteostasis regulation influences dormancy and fertility.
See below 2 calls👇
Reposted by André Dias
THANK YOU for many years of friendship, support and for sharing your great Science
La Pedrera #Barcelona
#NotInTheGenes #CellsRUs #Cambridge
La Pedrera #Barcelona
#NotInTheGenes #CellsRUs #Cambridge
September 22, 2025 at 9:01 PM
THANK YOU for many years of friendship, support and for sharing your great Science
La Pedrera #Barcelona
#NotInTheGenes #CellsRUs #Cambridge
La Pedrera #Barcelona
#NotInTheGenes #CellsRUs #Cambridge
Reposted by André Dias
Great opportunity to join the vibrant developmental biology in Toronto (and boss around me and @wbderry.bsky.social).
jobs.sciencecareers.org/job/673968/p...
jobs.sciencecareers.org/job/673968/p...
Program Head – Developmental, Stem Cell, and Cancer Biology - Toronto (Region), Ontario (CA) job with The Hospital for Sick Children (SickKids) Research Institute | 673968
Provide leadership and mentorship to members of the Developmental, Stem Cell & Cancer and influence the scientific direction of the Research Institute
jobs.sciencecareers.org
July 27, 2025 at 11:35 PM
Great opportunity to join the vibrant developmental biology in Toronto (and boss around me and @wbderry.bsky.social).
jobs.sciencecareers.org/job/673968/p...
jobs.sciencecareers.org/job/673968/p...
Reposted by André Dias
Catch up on Development presents… July webinar on early embryogenesis
Featuring talks from @lakshmib02.bsky.social, @dias-andre.bsky.social and @nikhil-mishra.bsky.social
🎥 thenode.biologists.com/catch-up-on-...
@dev-journal.bsky.social #DevBio
Featuring talks from @lakshmib02.bsky.social, @dias-andre.bsky.social and @nikhil-mishra.bsky.social
🎥 thenode.biologists.com/catch-up-on-...
@dev-journal.bsky.social #DevBio
Catch up on Development presents… July webinar on early embryogenesis - the Node
Catch up on the Development presents... May webinar, featuring talks from the first authors of Development's 2024 outstanding paper prize finalists.
thenode.biologists.com
July 14, 2025 at 8:56 AM
Catch up on Development presents… July webinar on early embryogenesis
Featuring talks from @lakshmib02.bsky.social, @dias-andre.bsky.social and @nikhil-mishra.bsky.social
🎥 thenode.biologists.com/catch-up-on-...
@dev-journal.bsky.social #DevBio
Featuring talks from @lakshmib02.bsky.social, @dias-andre.bsky.social and @nikhil-mishra.bsky.social
🎥 thenode.biologists.com/catch-up-on-...
@dev-journal.bsky.social #DevBio
Tune in tomorrow if you are interested in understanding how stem cells can be used to make different parts of our body
Sign up to attend our next Development presents... webinar on early #embryogenesis, featuring talks from @lakshmib02.bsky.social, @dias-andre.bsky.social and @nikhil-mishra.bsky.social
📅Wed 9 July 15:00BST (UTC+1)
Register here: us02web.zoom.us/webinar/regi...
#DevBio @dev-journal.bsky.social
📅Wed 9 July 15:00BST (UTC+1)
Register here: us02web.zoom.us/webinar/regi...
#DevBio @dev-journal.bsky.social
July 8, 2025 at 9:07 AM
Tune in tomorrow if you are interested in understanding how stem cells can be used to make different parts of our body
Reposted by André Dias
Sign up to attend our next Development presents... webinar on early #embryogenesis, featuring talks from @lakshmib02.bsky.social, @dias-andre.bsky.social and @nikhil-mishra.bsky.social.
📅Wed 9 July 15:00BST (UTC+1)
Register here: us02web.zoom.us/webinar/regi...
#DevBio @dev-journal.bsky.social
📅Wed 9 July 15:00BST (UTC+1)
Register here: us02web.zoom.us/webinar/regi...
#DevBio @dev-journal.bsky.social
June 23, 2025 at 8:26 AM
Sign up to attend our next Development presents... webinar on early #embryogenesis, featuring talks from @lakshmib02.bsky.social, @dias-andre.bsky.social and @nikhil-mishra.bsky.social.
📅Wed 9 July 15:00BST (UTC+1)
Register here: us02web.zoom.us/webinar/regi...
#DevBio @dev-journal.bsky.social
📅Wed 9 July 15:00BST (UTC+1)
Register here: us02web.zoom.us/webinar/regi...
#DevBio @dev-journal.bsky.social
Reposted by André Dias
In which we discuss the homologies between amphibian and mammalian organizers from the perspective of #gastruloids with special relevance to recently described A and C gastruloids as containing Spemann/Mangold and Trunk/Tail organizers www.sciencedirect.com/science/arti...
May 23, 2025 at 10:35 AM
In which we discuss the homologies between amphibian and mammalian organizers from the perspective of #gastruloids with special relevance to recently described A and C gastruloids as containing Spemann/Mangold and Trunk/Tail organizers www.sciencedirect.com/science/arti...
Reposted by André Dias
Experimental embryology postdoc available in my lab at the @biology.ox.ac.uk @ox.ac.uk working on the evolution of vertebral counts. Reach out if you’re passionate about EvoDevo, enjoy lab work and microscopy and are into or could get into cichlid fishes. Deadline on the 16th June. Please share!
May 19, 2025 at 4:10 PM
Experimental embryology postdoc available in my lab at the @biology.ox.ac.uk @ox.ac.uk working on the evolution of vertebral counts. Reach out if you’re passionate about EvoDevo, enjoy lab work and microscopy and are into or could get into cichlid fishes. Deadline on the 16th June. Please share!
We've just updated our manuscript to a more 'publishable' version!
www.biorxiv.org/content/10.1...
New details about the role of Wnt in suppressing neural differentiation and fostering a posterior epiblast state, and the temporal effect of Nodal signalling in driving mesendodermal fates
www.biorxiv.org/content/10.1...
New details about the role of Wnt in suppressing neural differentiation and fostering a posterior epiblast state, and the temporal effect of Nodal signalling in driving mesendodermal fates
May 16, 2025 at 7:06 AM
We've just updated our manuscript to a more 'publishable' version!
www.biorxiv.org/content/10.1...
New details about the role of Wnt in suppressing neural differentiation and fostering a posterior epiblast state, and the temporal effect of Nodal signalling in driving mesendodermal fates
www.biorxiv.org/content/10.1...
New details about the role of Wnt in suppressing neural differentiation and fostering a posterior epiblast state, and the temporal effect of Nodal signalling in driving mesendodermal fates
Great work from Alexandra and Sebastian et al showing how gastrulation is governed downstream of Wnt and Nodal. Two competing transcriptional modules - two opposing signalling activities - orchestrate the emergence of the mammalian body plan.
moving forward, together
moving forward, together
Two transcriptional modules that control mammalian #gastrulation that are functionally linked through their GRNs www.biorxiv.org/content/10.1... and paced by interactions between Wnt and Nodal signalling
Great collaboration with S Arnold and K McDole's labs #gastruloids
Great collaboration with S Arnold and K McDole's labs #gastruloids
May 16, 2025 at 6:34 AM
Great work from Alexandra and Sebastian et al showing how gastrulation is governed downstream of Wnt and Nodal. Two competing transcriptional modules - two opposing signalling activities - orchestrate the emergence of the mammalian body plan.
moving forward, together
moving forward, together
Reposted by André Dias
Difficult to believe that such a community has emerged @ #gastruloids as a model system to explore many aspects of #DevBio. Great to see a room so full of people, curiosity and good Science @prbb.org @upf.edu Thank you to all that have made this possible & of course to the participants
March 21, 2025 at 6:19 AM
Difficult to believe that such a community has emerged @ #gastruloids as a model system to explore many aspects of #DevBio. Great to see a room so full of people, curiosity and good Science @prbb.org @upf.edu Thank you to all that have made this possible & of course to the participants
Reposted by André Dias
There's increasing evidence that in organoid/embryoids conditions of cells at start of experiments is crucial/ determines outcome. Much to be learnt still. Taking advantage of mouse #gastruloids, two groups explore this here. Important work to calibrate the field www.biorxiv.org/content/10.1...
March 19, 2025 at 12:01 PM
There's increasing evidence that in organoid/embryoids conditions of cells at start of experiments is crucial/ determines outcome. Much to be learnt still. Taking advantage of mouse #gastruloids, two groups explore this here. Important work to calibrate the field www.biorxiv.org/content/10.1...
Reposted by André Dias
N2B27 media formulations influence gastruloid development https://www.biorxiv.org/content/10.1101/2025.03.15.643474v1
March 17, 2025 at 4:30 AM
N2B27 media formulations influence gastruloid development https://www.biorxiv.org/content/10.1101/2025.03.15.643474v1
Fun collaboration! All the nitty-gritty about N2B27 and gastruloids. Kudos to all the authors, particularly Tina Balayo (¡que va de primero!)
New Gastruloid preprint just landed... nice collaboration between our lab, @dias-andre.bsky.social, and @amartinezarias.bsky.social www.biorxiv.org/content/10.1...
N2B27 media formulations influence gastruloid development
Gastruloids are 3D aggregates of pluripotent stem cells grown in suspension culture that mimic many aspects of gastrulation and early axial elongation. The N2B27 basal medium in which mouse gastruloid...
www.biorxiv.org
March 17, 2025 at 12:09 PM
Fun collaboration! All the nitty-gritty about N2B27 and gastruloids. Kudos to all the authors, particularly Tina Balayo (¡que va de primero!)
Reposted by André Dias
🧫Looking for a PhD to start Autumn 2025? We're recruiting! 🧪
The project will focus on using #embryomodels to explore #development & could cover a range of specific questions. Please RT! 🙏
Apply via the portal, and get more details here: www.crick.ac.uk/careers-stud...
The project will focus on using #embryomodels to explore #development & could cover a range of specific questions. Please RT! 🙏
Apply via the portal, and get more details here: www.crick.ac.uk/careers-stud...
February 20, 2025 at 1:21 PM
🧫Looking for a PhD to start Autumn 2025? We're recruiting! 🧪
The project will focus on using #embryomodels to explore #development & could cover a range of specific questions. Please RT! 🙏
Apply via the portal, and get more details here: www.crick.ac.uk/careers-stud...
The project will focus on using #embryomodels to explore #development & could cover a range of specific questions. Please RT! 🙏
Apply via the portal, and get more details here: www.crick.ac.uk/careers-stud...
Reposted by André Dias
The Lanner lab is recruiting a postdoctoral fellow to continue developing our strategy to generate pluripotent stem cell derived photoreceptors for treatment of blindness. Looking for talented colleague to join our team.
@ki.se
ki.varbi.com/what:job/job...
@ki.se
ki.varbi.com/what:job/job...
Postdoktor för framställning av fotoreceptorer från pluripotenta stamceller
Vill du bidra till medicinsk forskning av toppkvalitet? Lanner labbet som tillhör institutionen för klinisk vetenskap, intervention och teknik har utvecklat en metod att producera retinala pigment e
ki.varbi.com
February 20, 2025 at 7:27 PM
The Lanner lab is recruiting a postdoctoral fellow to continue developing our strategy to generate pluripotent stem cell derived photoreceptors for treatment of blindness. Looking for talented colleague to join our team.
@ki.se
ki.varbi.com/what:job/job...
@ki.se
ki.varbi.com/what:job/job...
Reposted by André Dias
We are looking for a new Executive Editor on the journal, with @katherine-brown.bsky.social taking on a new role at @biologists.bsky.social. If you're an experienced editor with a love of developmental biology and its community, this could be the job for you. Full details in the link below.
www.biologists.com
February 13, 2025 at 1:56 PM
We are looking for a new Executive Editor on the journal, with @katherine-brown.bsky.social taking on a new role at @biologists.bsky.social. If you're an experienced editor with a love of developmental biology and its community, this could be the job for you. Full details in the link below.
Reposted by André Dias
I am recruiting an #ERC funded PostDoc to work on the evolution of vertebral counts in cichlids. This is an experimental project & will be looking for candidates with experience generating reporter lines, live-imaging and experimental embryology.
Application deadline: 25th Feb.
tinyurl.com/33jbu8fa
Application deadline: 25th Feb.
tinyurl.com/33jbu8fa
February 5, 2025 at 4:50 PM
I am recruiting an #ERC funded PostDoc to work on the evolution of vertebral counts in cichlids. This is an experimental project & will be looking for candidates with experience generating reporter lines, live-imaging and experimental embryology.
Application deadline: 25th Feb.
tinyurl.com/33jbu8fa
Application deadline: 25th Feb.
tinyurl.com/33jbu8fa