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deriverylab.bsky.social
Derivery Lab - MRC LMB
@deriverylab.bsky.social
820 followers 690 following 24 posts
Asymmetric cell division | Microscopy tech development | Synthetic biology | Fly models |

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deriverylab.bsky.social
During revisions, we've added lots of exciting new data, so be sure to check out the paper! Most importantly, we now demonstrate that Elongator regulates polyglutamylation levels of spindle microtubules, both in cells and in vivo.
Figure 5. Elongator is a tubulin polymerase selective for polyglutamylated monomers. (A, B) Elp456 binds preferentially to polyglutamylated tubulin. (A) Affinity measurement between eGFP-Elp456 and indicated αβ-tubulin heterodimers using microscale thermophoresis. Estimated dissociation constant (Kd) values are indicated (mean ± s.d.; n = 3). (B) Estimated dissociation constants for the indicated tubulins. Elp456 binds with similar affinity to recombinant Drosophila α1β1-tubulin, pig brain tubulin, mouse brain tubulin and mouse brain tubulin lacking K40 acetylation. However, the binding to tubulin purified from TTLL1/7-double KO mouse brains (lacking polyglutamylation) and HeLa S3 cells (lacking polyglutamylation, K40 acetylation and Δ2 deletion is similar and ~18 times lower than other tubulins. (C) Experimental assay to detect polyglutamylation in polymerized microtubules in vitro. (D) Microtubules polymerized in the presence of Elongator are significantly enriched in polyglutamylated tubulin when using a 50:50 pig brain tubulin:HeLa S3 tubulin mix. Correspondingly, when using HeLa S3 tubulin, which is not polyglutamylated, no effect is observed. P values for an Ordinary one-way ANOVA test followed by two-tailed Turkey multiple comparison test are indicated (E–G) Elongator depletion results in decreased polyglutamylation levels at the spindle. mean ± standard deviation. P values for an Ordinary one-way ANOVA test on independent experiments (N = 3) followed by two-tailed Turkey multiple comparison test are indicated. (E) Drosophila S2 cells depleted for Elongator have significantly decrease levels of polyglutamylated tubulin at the mitotic spindle.
January 17, 2025 at 9:46 AM
deriverylab.bsky.social
In the original draft, we showed that Elongator binds to microtubules irrespective of their PTMs, but selectively elongates them using polyglutamylated tubulin subunits. As a result, Elongator rewrites the tubulin code of dynamic microtubules. How cool is that?
Elongator, an established tRNA-modifying complex, can also stabilize microtubules during asymmetric cell division. This study shows that Elongator subcomplexes achieve this by binding to microtubule ends as well as tubulin dimers, and enriching microtubules with polyglutamylated tubulin. • Dual binding mechanism: Elongator binds microtubule ends and free αβ-tubulin heterodimers via its Elp123 and Elp456 subcomplexes, respectively. • Microtubule stabilization: Coupled activity of Elp123 and Elp456 reduces the tubulin concentration required for microtubule elongation, increasing microtubule growth speed and reducing their catastrophe rate. • Selective tubulin recognition: Elp456 binds with higher affinity to polyglutamylated tubulin, resulting in preferential incorporation of polyglutamylated tubulin into Elongator-assembled microtubules. • Tubulin code rewriting: By enriching microtubules with polyglutamylated tubulin, Elongator modifies the tubulin code in vitro, in cells and in vivo in Drosophila sensory organ precursor cells.
January 17, 2025 at 9:46 AM
deriverylab.bsky.social
An overview of Akaash's multispectral camera tree can be seen in the graphical abstract (which you can read more about here: www.physbiol.cam.ac.uk/prizes ). Peek at some colourful cells!
‪Derivery Lab - MRC LMB‬ ‪@deriverylab.bsky.social‬ · 3m Congrats to Akaash, a postdoc in the lab, for winning the PhD Award from the Cambridge Centre for Physical Biology. His PhD research worked on the development of a multispectral imaging system capable of imaging up to 8 fluorophores similtaneously and tackling complex biological questions with this! Photograph of Akaash in posed infront of a computer monitor with a colourful cell imaged using his multispectral camera tree which is to the right of him. ALT Derivery Lab - MRC LMB ‪@deriverylab.bsky.social‬ An overview of Akaash's multispectral camera tree can be seen in the graphical abstract (which you can read more about here: www.physbiol.cam.ac.uk/prizes ). Peek at some colourful cells! 1a) Schematic of multispectral imaging hardware, b) photo of multispectral imaging hardware. 2a) Raw recorded data, b) emission spectra of fluorophores used in sample, c) unmixed data using novel algorithm, d) merge of data in c, e-f) other examples of simultaneous multispectral live cell imaging ALT December 11, 2024 at 11:20 AM Home Search Notifications Chat Feeds Lists Profile Settings Getting started Like 10 posts Teach our algorithm what you like Follow 7 accounts Bluesky is better with friends! Discover Following Extracellular Vesicles More feeds Feedback · Privacy · Terms · Help 1a) Schematic of multispectral imaging hardware, b) photo of multispectral imaging hardware. 2a) Raw recorded data, b) emission spectra of fluorophores used in sample, c) unmixed data using novel algorithm, d) merge of data in c, e-f) other examples of simultaneous multispectral live cell imaging
December 11, 2024 at 11:24 AM
deriverylab.bsky.social
And now we welcome our new PhD student @paddyod.bsky.social !
November 22, 2024 at 1:24 PM
deriverylab.bsky.social
Then, only last week, post-doc @vicenteph.bsky.social was promoted to Senior Investigator Scientist and will remain in the group to develop new methods in protein design, in vitro reconstitutions, multispectral imaging assays and his work on Elongator!
Vicente stands behind a table holding a dinosaur plush toy. In front of him is a table which contains the blue-green box from which the dinosaur plush appeared, and a minions celebration cake which has dinosaur decorations on cocktail sticks on top of it.
November 22, 2024 at 1:23 PM
deriverylab.bsky.social
Later in October, the LMB had their yearly annual symposium with Post-doc Lara Kruger winning the Steitz Prize for her talk and @akaashkumar.bsky.social winning the Perutz Prize for his PhD work!
On the bottom left is Akaash Kumar, bottom right is Lara Kruger, behind lara is Manu Derivery and behind Akaash is James Manton, all holding glasses of champagne.
November 22, 2024 at 1:23 PM
deriverylab.bsky.social
Then, in September @akaashkumar.bsky.social passed his PhD viva, becoming the latest doctor to emerge from the lab! He then won the RMS Early Career Award 2024 in October.
Akaash stands in front of some stairs, holding a bottle of champagne whilst wearing a suit.
November 22, 2024 at 1:23 PM
deriverylab.bsky.social
In June, @akaashkumar.bsky.social and @jamesdmanton.bsky.social had their Multispectral live-cell imaging paper released on to the BioRxiv www.biorxiv.org/content/10.1...
Multispectral live-cell imaging with uncompromised spatiotemporal resolution
Multispectral imaging is an established method to extend the number of colours usable in fluorescence imaging beyond the typical limit of three or four, but standard approaches are poorly suited to li...
www.biorxiv.org
November 22, 2024 at 1:23 PM