Whew the most amazing I've ever seen the sea. Off the coast of Kerry, Ireland

Giant fiancée took me out for my nearly-birthday

Looking forward to the Spa GP this weekend. Ferrari sharing some cool artwork this week

Great evening of talks at #pint25 in Oxford Tech Me Out theme. Very interesting talks from Alex and Jorge from @alpinef1.bsky.social followed by Muhammed Waqas from JLB.

Alpine even brought a front wing from their 2024 F1 car!
@pintofscience.uk

Great weekend celebrating @emmarose64.bsky.social's graduation with friends and family. Very proud! 😊

Some great images in this years @the-node.bsky.social image competition. Including my own "Thymus in the Spotlight" image taken in the @idrm.ox.ac.uk Zeiss CoE.

Vote for your favorite here: thenode.biologists.com/vote-for-you...

Taking @emmarose64.bsky.social and my mum to their live first rugby games was great. Especially with it being such a good game and Gloucester winning 🍒⚪

Finally, our proposed model showing LEC differentiation through in situ specification from ETV2+PROX1+ paraxial mesoderm-derived lymphangioblasts

HCR (@hcrimaging.bsky.social) stainings suggest VEGFC/VEGFR3 signaling acts upstream of PROX1

In the absence of VEGFC/VEGFR3 signaling, specification of LECs from PXM stalls at the angioblast stage with Vegfc-/- embryos having more Etv2+Prox1- cells in the HDM.

If migrating venous ECs are the major source of LECs, a substantial increase in EC numbers would be required to maintain venous integrity.

Analysis of cell cycle phase suggests angioblast-derived LECs and not venous ECs, are proliferative enough to support the rapid expansion of LECs.

Conditional lineage tracing analyses using an inducible Pax3-CreERT2 line further highlighted the transient nature of this progenitor source, and demonstrated that lymphangioblasts give rise to LECs in various tissues.

To better characterise lymphangioblast specification from PXM we performed snMultiome (RNA & ATAC) on Pax3-lineage progenitors and ECs at E9.5.

SCENIC+ analysis showed GRNs formed by known (ETV2/SOX18) and novel (EBF1/2) regulators of EC/LEC specification & differentiation in lymphangioblasts

IF and HCR (@hcrimaging.bsky.social) staining of wholemount and vibratome sectioned E9.5 embryos identified lymphangioblasts as residing immediately dorsal to the cardinal vein in the Lbx1+ hypaxial dermomyotome in a pattern reminiscent of LECs migrating from the cardinal vein

Surprisingly, we identified a population of cells that express both the angioblast marker Etv2 and the master regulator of LEC fate, Prox1. We have termed these cells lymphangioblasts.

The prevailing model was that PXM-derived cells contribute to venous endothelium and then transdifferentiate to LECs

Irina Lupu's postdoc in Ollie's newly established lab aimed to define molecular features that may drive PXM's LEC competence using scRNA-seq on sorted EC and progenitor populations

I'm not saying I spend too much time on the microscope but I can't help seeing blood vessels in christmas lights