David
@dav1dcg.bsky.social
All about neuronal activity: development, visual cortex, neuromodulation, interneurons, patch-clamp, and in vivo calcium imaging.
Neuroscience blog: https://spikesandbursts.wordpress.com/
Neuroscience blog: https://spikesandbursts.wordpress.com/
Reposted by David
Time, space, memory and brain–body rhythms — a Perspective by György Buzsáki
#neuroscience #neuroskyence
www.nature.com/articles/s41...
#neuroscience #neuroskyence
www.nature.com/articles/s41...
Time, space, memory and brain–body rhythms - Nature Reviews Neuroscience
Understanding how the brain represents experienced time and how representations of space and time are integrated to form episodic memories has been a goal of much neuroscientific research. In this Per...
www.nature.com
November 13, 2025 at 11:43 AM
Time, space, memory and brain–body rhythms — a Perspective by György Buzsáki
#neuroscience #neuroskyence
www.nature.com/articles/s41...
#neuroscience #neuroskyence
www.nature.com/articles/s41...
REM sleep reduces subthreshold depolarization in cortical pyramidal neurons in a region-specific manner: doi.org/10.1016/j.ce...
"NMDA receptors drive subthreshold depolarization specifically during REM sleep."
"NMDA receptors drive subthreshold depolarization specifically during REM sleep."
November 9, 2025 at 8:56 PM
REM sleep reduces subthreshold depolarization in cortical pyramidal neurons in a region-specific manner: doi.org/10.1016/j.ce...
"NMDA receptors drive subthreshold depolarization specifically during REM sleep."
"NMDA receptors drive subthreshold depolarization specifically during REM sleep."
Continuous cell-type diversification in mouse visual cortex development
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Continuous cell-type diversification in mouse visual cortex development - Nature
A high-resolution transcriptomic and epigenomic cell-type atlas of the developing mouse visual cortex from embryonic to postnatal development is presented, providing a real-time dynamic molecular map ...
www.nature.com
November 9, 2025 at 8:52 PM
Continuous cell-type diversification in mouse visual cortex development
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by David
More powerful tools for understanding the brain. 🧠🔍
Today, comprehensive, cross-species developing brain cell atlases were released to help advance health and disease research. 🧵
#studyBRAIN #BICAN
Today, comprehensive, cross-species developing brain cell atlases were released to help advance health and disease research. 🧵
#studyBRAIN #BICAN
November 5, 2025 at 5:45 PM
More powerful tools for understanding the brain. 🧠🔍
Today, comprehensive, cross-species developing brain cell atlases were released to help advance health and disease research. 🧵
#studyBRAIN #BICAN
Today, comprehensive, cross-species developing brain cell atlases were released to help advance health and disease research. 🧵
#studyBRAIN #BICAN
Reposted by David
First neurons didn’t appear overnight. We trace their roots to ancient secretory cells - showing how lifestyle & behavior shaped the evolution of first synapses.🧠🌊 #Evolution #Neuroscience
Our latest in @natrevneuro.nature.com
Link: rdcu.be/eMX3E
@jeffcolgren.bsky.social @msarscentre.bsky.social
Our latest in @natrevneuro.nature.com
Link: rdcu.be/eMX3E
@jeffcolgren.bsky.social @msarscentre.bsky.social
The evolutionary origins of synaptic proteins and their changing roles in different organisms across evolution
Nature Reviews Neuroscience - Recent studies have shed further light on the evolutionary origins of chemical synapses, In this Review, Colgren and Burkhardt explore how ancient proteins were...
rdcu.be
October 27, 2025 at 6:48 PM
First neurons didn’t appear overnight. We trace their roots to ancient secretory cells - showing how lifestyle & behavior shaped the evolution of first synapses.🧠🌊 #Evolution #Neuroscience
Our latest in @natrevneuro.nature.com
Link: rdcu.be/eMX3E
@jeffcolgren.bsky.social @msarscentre.bsky.social
Our latest in @natrevneuro.nature.com
Link: rdcu.be/eMX3E
@jeffcolgren.bsky.social @msarscentre.bsky.social
Illuminating calcium and potassium dynamics with red fluorescent sensors
journals.plos.org/plosbiology/...
journals.plos.org/plosbiology/...
Illuminating calcium and potassium dynamics with red fluorescent sensors
Despite recent advances in green genetically encoded ion indicators, developments in red-shifted indicators have been slower. This Primer explores two recent studies in PLOS Biology that engineer red ...
journals.plos.org
October 26, 2025 at 9:25 PM
Illuminating calcium and potassium dynamics with red fluorescent sensors
journals.plos.org/plosbiology/...
journals.plos.org/plosbiology/...
Reposted by David
That merely being “under review” by a Nature family journal is offered as a quality proxy for a paper is a tragic illustration of the extent to which academia is addicted to brands and outsources evaluation
October 25, 2025 at 6:37 PM
That merely being “under review” by a Nature family journal is offered as a quality proxy for a paper is a tragic illustration of the extent to which academia is addicted to brands and outsources evaluation
Reposted by David
TT faculty job opening in #Neuroscience!
We are looking for a colleague to join us in our fantastic Biology Department and Neuroscience Program at Brandeis. We are a group of *very* collaborative, supportive, and productive scientists (& humans!) so please apply
academicjobsonline.org/ajo/jobs/30961
We are looking for a colleague to join us in our fantastic Biology Department and Neuroscience Program at Brandeis. We are a group of *very* collaborative, supportive, and productive scientists (& humans!) so please apply
academicjobsonline.org/ajo/jobs/30961
Brandeis University, Biology Department
Job #AJO30961, Assistant Professor in Biology and Neuroscience Program, Biology Department, Brandeis University, Waltham, Massachusetts, US
academicjobsonline.org
October 22, 2025 at 11:28 PM
TT faculty job opening in #Neuroscience!
We are looking for a colleague to join us in our fantastic Biology Department and Neuroscience Program at Brandeis. We are a group of *very* collaborative, supportive, and productive scientists (& humans!) so please apply
academicjobsonline.org/ajo/jobs/30961
We are looking for a colleague to join us in our fantastic Biology Department and Neuroscience Program at Brandeis. We are a group of *very* collaborative, supportive, and productive scientists (& humans!) so please apply
academicjobsonline.org/ajo/jobs/30961
In case someone finds it more useful, I am starting to upload the code from the blog’s patch-clamp tutorials to GitHub. Check it out!
github.com/dav1dcg/spik...
github.com/dav1dcg/spik...
GitHub - dav1dcg/spikesandbursts: Code from the neuroscience blog Spikes and Bursts
Code from the neuroscience blog Spikes and Bursts. Contribute to dav1dcg/spikesandbursts development by creating an account on GitHub.
github.com
October 19, 2025 at 6:00 PM
In case someone finds it more useful, I am starting to upload the code from the blog’s patch-clamp tutorials to GitHub. Check it out!
github.com/dav1dcg/spik...
github.com/dav1dcg/spik...
Modulation of speed-dependent acetylcholine release in the hippocampus by spatial task engagement: www.cell.com/cell-reports...
October 19, 2025 at 5:59 PM
Modulation of speed-dependent acetylcholine release in the hippocampus by spatial task engagement: www.cell.com/cell-reports...
Distinct representations of economic variables across regions and projections of the frontal cortex: www.cell.com/neuron/fullt...
Task for expected value and risk associated with reward-predicting stimuli. Stronger expected-value coding in dorsal cortex, risk coding in medial cortex.
Task for expected value and risk associated with reward-predicting stimuli. Stronger expected-value coding in dorsal cortex, risk coding in medial cortex.
October 19, 2025 at 5:57 PM
Distinct representations of economic variables across regions and projections of the frontal cortex: www.cell.com/neuron/fullt...
Task for expected value and risk associated with reward-predicting stimuli. Stronger expected-value coding in dorsal cortex, risk coding in medial cortex.
Task for expected value and risk associated with reward-predicting stimuli. Stronger expected-value coding in dorsal cortex, risk coding in medial cortex.
Rapid adaptive optics enabling near noninvasive high-resolution brain imaging in awake behaving mice
Under 2P excitation, adaptive optics measures the aberrated point spread function in ≈0.1 s/measurement, compensating for aberrations and scattering.
www.nature.com/articles/s41...
Under 2P excitation, adaptive optics measures the aberrated point spread function in ≈0.1 s/measurement, compensating for aberrations and scattering.
www.nature.com/articles/s41...
Rapid adaptive optics enabling near noninvasive high-resolution brain imaging in awake behaving mice - Nature Communications
The authors present the adaptive optics system MD-FSS, which allows high-resolution brain imaging in awake mice with rapid aberrations correction against motion. It reveals differences in brain functi...
www.nature.com
October 19, 2025 at 5:49 PM
Rapid adaptive optics enabling near noninvasive high-resolution brain imaging in awake behaving mice
Under 2P excitation, adaptive optics measures the aberrated point spread function in ≈0.1 s/measurement, compensating for aberrations and scattering.
www.nature.com/articles/s41...
Under 2P excitation, adaptive optics measures the aberrated point spread function in ≈0.1 s/measurement, compensating for aberrations and scattering.
www.nature.com/articles/s41...
Reposted by David
As ever, beautiful #cryoEM work from the lab of Hong Zhou with field-defining insights using #cryoET:
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling
Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...
www.science.org
October 16, 2025 at 10:34 PM
As ever, beautiful #cryoEM work from the lab of Hong Zhou with field-defining insights using #cryoET:
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
Head-direction cells as a neural compass in bats navigating outdoors on a remote oceanic island
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Head-direction cells as a neural compass in bats navigating outdoors on a remote oceanic island
Animals and humans rely on their navigation skills to survive. However, spatial neurons in the brain’s “navigation circuit” had not previously been studied under real-world conditions. We conducted an...
www.science.org
October 19, 2025 at 5:42 PM
Head-direction cells as a neural compass in bats navigating outdoors on a remote oceanic island
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by David
Novel object recognition task for mice: Is it a test for memory, object neophobia or innate preference?
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Novel object recognition task for mice: Is it a test for memory, object neophobia or innate preference?
Novel object recognition task (NOR) is one of the most widely used memory tests for mice. Over the years of intensive use, the test protocol has becom…
www.sciencedirect.com
October 15, 2025 at 9:25 AM
Novel object recognition task for mice: Is it a test for memory, object neophobia or innate preference?
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Transient juvenile demyelination impairs maturation and function of parvalbumin-positive interneurons in the prefrontal cortex
Demyelination impaired high-frequency firing in PV interneurons associated with a substantial decrease in Kv3-specific currents.
doi.org/10.1371/jour...
Demyelination impaired high-frequency firing in PV interneurons associated with a substantial decrease in Kv3-specific currents.
doi.org/10.1371/jour...
Transient juvenile demyelination impairs maturation and function of parvalbumin-positive interneurons in the prefrontal cortex
What role does myelination play in the development of parvalbumin-positive (PV) interneurons in the prefrontal cortex? This study shows that juvenile demyelination disrupts PV interneuron firing and s...
doi.org
October 5, 2025 at 8:23 PM
Transient juvenile demyelination impairs maturation and function of parvalbumin-positive interneurons in the prefrontal cortex
Demyelination impaired high-frequency firing in PV interneurons associated with a substantial decrease in Kv3-specific currents.
doi.org/10.1371/jour...
Demyelination impaired high-frequency firing in PV interneurons associated with a substantial decrease in Kv3-specific currents.
doi.org/10.1371/jour...
Ultra-high-density Neuropixels probes improve detection and identification in neuronal recordings: www.cell.com/neuron/fullt...
6 μm site-to-site spacing.
Cortical cell types could be discriminated from one another with ∼80% accuracy and from other neurons with ∼85% accuracy.
6 μm site-to-site spacing.
Cortical cell types could be discriminated from one another with ∼80% accuracy and from other neurons with ∼85% accuracy.
October 5, 2025 at 8:15 PM
Ultra-high-density Neuropixels probes improve detection and identification in neuronal recordings: www.cell.com/neuron/fullt...
6 μm site-to-site spacing.
Cortical cell types could be discriminated from one another with ∼80% accuracy and from other neurons with ∼85% accuracy.
6 μm site-to-site spacing.
Cortical cell types could be discriminated from one another with ∼80% accuracy and from other neurons with ∼85% accuracy.
All-optical voltage interrogation for probing synaptic plasticity in vivo
New JEDI-2Psub GEVI to record in Purkinje cell dendrites while selectively activating their granule cell inputs using optogenetics and their climbing fiber inputs using sensory stimulation.
www.nature.com/articles/s41...
New JEDI-2Psub GEVI to record in Purkinje cell dendrites while selectively activating their granule cell inputs using optogenetics and their climbing fiber inputs using sensory stimulation.
www.nature.com/articles/s41...
All-optical voltage interrogation for probing synaptic plasticity in vivo - Nature Communications
Reliable measuring the voltage dynamics of individual neurons in the intact brain is significantly challenging. Here authors developed an all-optical method combining two-photon voltage imaging and optogenetics to measure and induce synaptic plasticity in vivo, revealing LTP of inhibition in cerebellar circuits and providing a blueprint to link synaptic changes to learning.
www.nature.com
October 5, 2025 at 8:10 PM
All-optical voltage interrogation for probing synaptic plasticity in vivo
New JEDI-2Psub GEVI to record in Purkinje cell dendrites while selectively activating their granule cell inputs using optogenetics and their climbing fiber inputs using sensory stimulation.
www.nature.com/articles/s41...
New JEDI-2Psub GEVI to record in Purkinje cell dendrites while selectively activating their granule cell inputs using optogenetics and their climbing fiber inputs using sensory stimulation.
www.nature.com/articles/s41...
Higher-order interactions in neuronal function: From genes to ionic currents in biophysical models
Models in this study show that "a small subset of ion channel genes captures essential variability across neuronal subtypes".
www.pnas.org/doi/10.1073/...
Models in this study show that "a small subset of ion channel genes captures essential variability across neuronal subtypes".
www.pnas.org/doi/10.1073/...
Higher-order interactions in neuronal function: From genes to ionic currents in biophysical models | PNAS
Neuronal firing patterns are the consequence of precise variations in neuronal membrane
potential, which are themselves shaped by multiple ionic cu...
www.pnas.org
October 5, 2025 at 8:04 PM
Higher-order interactions in neuronal function: From genes to ionic currents in biophysical models
Models in this study show that "a small subset of ion channel genes captures essential variability across neuronal subtypes".
www.pnas.org/doi/10.1073/...
Models in this study show that "a small subset of ion channel genes captures essential variability across neuronal subtypes".
www.pnas.org/doi/10.1073/...
Sensory origin of visually evoked activity in auditory cortex: www.cell.com/cell-reports...
Visual stimuli rarely evoke face movements.
Primary auditory cortex responses to visual input remain robust even in the absence of face movement, and optogenetic silencing of V1 reduces A1 visual responses.
Visual stimuli rarely evoke face movements.
Primary auditory cortex responses to visual input remain robust even in the absence of face movement, and optogenetic silencing of V1 reduces A1 visual responses.
October 5, 2025 at 7:56 PM
Sensory origin of visually evoked activity in auditory cortex: www.cell.com/cell-reports...
Visual stimuli rarely evoke face movements.
Primary auditory cortex responses to visual input remain robust even in the absence of face movement, and optogenetic silencing of V1 reduces A1 visual responses.
Visual stimuli rarely evoke face movements.
Primary auditory cortex responses to visual input remain robust even in the absence of face movement, and optogenetic silencing of V1 reduces A1 visual responses.
Added a new notebook 'dataframes_plots' with simple examples for navigating tables, plotting, and statistics in Python.
github.com/dav1dcg/neur...
github.com/dav1dcg/neur...
GitHub - dav1dcg/neuro-notebook-templates: Jupyter notebook templates for processing and analyzing neuroscience data.
Jupyter notebook templates for processing and analyzing neuroscience data. - dav1dcg/neuro-notebook-templates
github.com
September 29, 2025 at 5:59 PM
Added a new notebook 'dataframes_plots' with simple examples for navigating tables, plotting, and statistics in Python.
github.com/dav1dcg/neur...
github.com/dav1dcg/neur...
Reposted by David
Our paper on 'mini analysis' is now published in @jphysiol.bsky.social
If you are recording mPSCs or sPSCs, I hope this helps with analysis. Interpretation of these datasets is not as easy as it seems..!
Happy to discuss if you are interested
physoc.onlinelibrary.wiley.com/doi/10.1113/...
If you are recording mPSCs or sPSCs, I hope this helps with analysis. Interpretation of these datasets is not as easy as it seems..!
Happy to discuss if you are interested
physoc.onlinelibrary.wiley.com/doi/10.1113/...
‘Mini analysis’ misrepresents changes in synaptic properties due to incomplete event detection
Abstract figure legend Summary of the study where simulated recordings (left) were used to characterise the effect of incomplete detection on mini (mPSC) analysis. Recording noise levels (red) determ...
physoc.onlinelibrary.wiley.com
September 29, 2025 at 11:54 AM
Our paper on 'mini analysis' is now published in @jphysiol.bsky.social
If you are recording mPSCs or sPSCs, I hope this helps with analysis. Interpretation of these datasets is not as easy as it seems..!
Happy to discuss if you are interested
physoc.onlinelibrary.wiley.com/doi/10.1113/...
If you are recording mPSCs or sPSCs, I hope this helps with analysis. Interpretation of these datasets is not as easy as it seems..!
Happy to discuss if you are interested
physoc.onlinelibrary.wiley.com/doi/10.1113/...
A Critical Look at Critical Periods
Great review of the critical period in the visual cortex. Nice evolutionary perspective, covering the key papers and mechanisms, and proposing a continuous process of plasticity that already starts before eye opening.
www.annualreviews.org/content/jour...
Great review of the critical period in the visual cortex. Nice evolutionary perspective, covering the key papers and mechanisms, and proposing a continuous process of plasticity that already starts before eye opening.
www.annualreviews.org/content/jour...
A Critical Look at Critical Periods | Annual Reviews
Over the past decade and a half, a new understanding has emerged of the role of vision during the critical period in the primary visual cortex. Rather than driving competition for cortical space, visi...
www.annualreviews.org
September 28, 2025 at 7:58 PM
A Critical Look at Critical Periods
Great review of the critical period in the visual cortex. Nice evolutionary perspective, covering the key papers and mechanisms, and proposing a continuous process of plasticity that already starts before eye opening.
www.annualreviews.org/content/jour...
Great review of the critical period in the visual cortex. Nice evolutionary perspective, covering the key papers and mechanisms, and proposing a continuous process of plasticity that already starts before eye opening.
www.annualreviews.org/content/jour...
A real-time all-optical interface for dynamic perturbation of neural activity during behavior: www.sciencedirect.com/science/arti...
Real-time interface that integrates CaImAn for online calcium imaging analysis, and a custom hologram program for two-photon optogenetic stimulation.
Real-time interface that integrates CaImAn for online calcium imaging analysis, and a custom hologram program for two-photon optogenetic stimulation.
September 28, 2025 at 7:42 PM
A real-time all-optical interface for dynamic perturbation of neural activity during behavior: www.sciencedirect.com/science/arti...
Real-time interface that integrates CaImAn for online calcium imaging analysis, and a custom hologram program for two-photon optogenetic stimulation.
Real-time interface that integrates CaImAn for online calcium imaging analysis, and a custom hologram program for two-photon optogenetic stimulation.