Intestinal villus cells extrude living, following a calcium pulse. Unlike from extruding apoptotic cells, Ca2+ messaging stops at the first neighbors! #FluorescenceFriday

Part of our TrepatLab preprint: www.biorxiv.org/content/10.1...

Video by @ronjahoutekamer.bsky.social @gloerichlab.bsky.social

Great to see our conclusions align with the beautiful work from @marija-matejcic.bsky.social in the Trepat lab 3D force mapping, Ca²⁺-triggered myosin-IIA meshwork dissolution, and neighbour lamellipodia generating traction. Great synergy, highlighting the role of tension in extrusion regulation.

Thanks for highlighting this! My background in fly morphogenesis shaped how I think about tissue forces, their interplay with signaling, and experimental design. An experience I’d wholeheartedly recommend — Drosophila remains a rich source of inspiration, continuing to reveal fundamental concepts.

This is very complementary with the recent preprint of @xaviertrepat.bsky.social and Maria Matejcic using 2D organoids and providing 3D mechanical characterisation of the process

Important story dissecting the mechanism of gut cell extrusion using organoids. Local heterogeneity of tension promotes live extrusion and basal relaxation is sufficient to kick the cell out. Nice combination of optogenetics and live imaging to demonstrate this
www.science.org/doi/abs/10.1...

👉 Check out our key findings in this detailed thread by Hans Clevers.

Out in @science.org! We found that the intestine doesn’t just push out cells when crowded. Instead, cells are in a mechanical tug-of-war, and weaker ones are extruded. A new view of how our gut keeps its balance and stays healthy.
www.science.org/doi/10.1126/...

📣 It is my pleasure to announce our recent review with Margherita on the current approaches, challenges, and perspectives in studying human maternal-fetal interface using in vitro models www.cell.com/cell-stem-ce... 🚀✨️

Jeey! @nfdi4bioimage.bsky.social published their #RDM-related postcards FAIRly (made by Henning Falk, licensed CC-BY 4.0) 🔬💾😍
github.com/NFDI4BIOIMAG...

First time that my cartoons are printed on postcards! Have a look 👇

Exciting study by Amanda Andersson Rolf et al: In vitro expansion of a human fetal #pancreas #stemcell that generates all three pancreatic cell lineages”. www.cell.com/cell/fulltex...
New #organoid protocol allows the seemingly indefinite expansion of human fetal pancreatic Lgr5+ stem cell 1/

"The state of German-Polish relations is a European tragedy in the same way that the Warschauer Kniefall was a moment of European reconciliation."
Brilliant analysis by @JeremyCliffe and also an important read before the Polish elections this weekend.

There is so much at stake.

Anhören!
Wie sich große branchenfremde Investoren (ALDI, Immobilien etc) riesengroße Ackerflächen 🚜 in Ostdeutschland kaufen.
Am Staat vorbei, ohne Steuern zu zahlen, mit dem Segen des Bauernverbandes und zum Schaden der kleinen Landwirtschaftsbetriebe.
👇

Jugendarbeitslosigkeit in der EU, August 2023:

🇪🇸26.8%
🇪🇪23.5%
🇬🇷22.5%
🇷🇴22.3%
🇮🇹22.0%
🇸🇪21.1%
🇵🇹20.3%
🇭🇷20.0%
🇸🇰19.8%
🇱🇺19.2%
🇨🇾16.3%
🇫🇮16.1%
🇫🇷16.0%
🇧🇪14.2%
🇪🇺14.0%
🇭🇺12.8%
🇧🇬11.8%
🇩🇰11.6%
🇦🇹11.4%
🇮🇪11.2%
🇵🇱11.1%
🇸🇮10.4%
🇱🇻9.0%
🇲🇹9.0%
🇳🇱8.7%
🇱🇹8.6%
🇨🇿8.5%
🇩🇪5.7%

via: ec.europa.eu/eurostat/doc...

BlueSkye, let's go!