Constantinos Patinios
@copabio.bsky.social
Group leader at VU LSC-EMBL PI for Genome Editing Technologies, Vilnius, Lithuania
Genome engineer
CRISPR-Cas
DNA replication stall and repair
Genome engineer
CRISPR-Cas
DNA replication stall and repair
Thank you Jens
September 7, 2025 at 9:24 PM
Thank you Jens
Thank you Nicholas 😊
September 5, 2025 at 9:40 AM
Thank you Nicholas 😊
Amazing Martin!! All the best with the new lab and the amazing research you do!!!
September 5, 2025 at 9:39 AM
Amazing Martin!! All the best with the new lab and the amazing research you do!!!
Big shout-out to first authors Darshana Gupta, (myself :) ) & Harris V. Bassett, senior author Chase L. Beisel, and project initiator Scott P. Collins 🙌
September 4, 2025 at 6:51 PM
Big shout-out to first authors Darshana Gupta, (myself :) ) & Harris V. Bassett, senior author Chase L. Beisel, and project initiator Scott P. Collins 🙌
This opens up brand-new possibilities for precision editing and studying DNA repair!
September 4, 2025 at 6:51 PM
This opens up brand-new possibilities for precision editing and studying DNA repair!
We developed append editing, a genome-editing strategy that installs ADP-ribose groups onto DNA to unlock new repair outcomes:
🔹 In bacteria → precise, scar-free edits via homologous recombination
🔹 In eukaryotes → unique T→A or T→C base substitutions not possible with current editors
🔹 In bacteria → precise, scar-free edits via homologous recombination
🔹 In eukaryotes → unique T→A or T→C base substitutions not possible with current editors
September 4, 2025 at 6:51 PM
We developed append editing, a genome-editing strategy that installs ADP-ribose groups onto DNA to unlock new repair outcomes:
🔹 In bacteria → precise, scar-free edits via homologous recombination
🔹 In eukaryotes → unique T→A or T→C base substitutions not possible with current editors
🔹 In bacteria → precise, scar-free edits via homologous recombination
🔹 In eukaryotes → unique T→A or T→C base substitutions not possible with current editors
Learn more ➡️ www.gmc.vu.lt/en/lsc-embl/...
The Laboratory of Dr. Constantinos Patinios
VU Gyvybės mokslų centras jungia tris VU padalinius – Biomokslų, Biotechnologijos ir Biochemijos institutus. Centras įkurtas vystant integruotą mokslo, studijų ir verslo centrą (slėnį) „Saulėtekis“.
www.gmc.vu.lt
May 7, 2025 at 3:26 PM
Learn more ➡️ www.gmc.vu.lt/en/lsc-embl/...
Deadline: Open until a suitable candidate is found.
📧 Send your CV + Motivation Letter to: [email protected]
📧 Send your CV + Motivation Letter to: [email protected]
May 7, 2025 at 3:26 PM
Deadline: Open until a suitable candidate is found.
📧 Send your CV + Motivation Letter to: [email protected]
📧 Send your CV + Motivation Letter to: [email protected]
🧬 Our work focuses on next-generation genome editing tools for bacteria, yeasts, and phages — with a special focus on DNA replication stalling and repair!
May 7, 2025 at 3:26 PM
🧬 Our work focuses on next-generation genome editing tools for bacteria, yeasts, and phages — with a special focus on DNA replication stalling and repair!
🌍 Based in beautiful Vilnius, Lithuania
🔬 Access to state-of-the-art equipment and facilities
💼 Competitive salary, personalized mentorship, and cutting-edge research!
🔬 Access to state-of-the-art equipment and facilities
💼 Competitive salary, personalized mentorship, and cutting-edge research!
May 7, 2025 at 3:26 PM
🌍 Based in beautiful Vilnius, Lithuania
🔬 Access to state-of-the-art equipment and facilities
💼 Competitive salary, personalized mentorship, and cutting-edge research!
🔬 Access to state-of-the-art equipment and facilities
💼 Competitive salary, personalized mentorship, and cutting-edge research!
If you’re passionate about genome editing or synthetic biology…
If you want tailored mentorship to drive independent research and launch your career…
This is the place for you! ✨
If you want tailored mentorship to drive independent research and launch your career…
This is the place for you! ✨
May 7, 2025 at 3:26 PM
If you’re passionate about genome editing or synthetic biology…
If you want tailored mentorship to drive independent research and launch your career…
This is the place for you! ✨
If you want tailored mentorship to drive independent research and launch your career…
This is the place for you! ✨
Huge thanks to Simona Della Valle, Enrico Orsi and Sjoerd Creutzburg for driving this work.
Happy editing :)
Happy editing :)
November 25, 2024 at 1:47 PM
Huge thanks to Simona Della Valle, Enrico Orsi and Sjoerd Creutzburg for driving this work.
Happy editing :)
Happy editing :)
If you want to use SIBR2.0 for your gene/protein of interest, you can visit the SIBR Site Finder site: colab.research.google.com/drive/162gIZ...
Google Colab
colab.research.google.com
November 25, 2024 at 1:47 PM
If you want to use SIBR2.0 for your gene/protein of interest, you can visit the SIBR Site Finder site: colab.research.google.com/drive/162gIZ...
SIBR2.0 can virtually control the expression of any gene/protein of interest, in a wide range of (if not all) mesophilic prokaryotes
November 25, 2024 at 1:47 PM
SIBR2.0 can virtually control the expression of any gene/protein of interest, in a wide range of (if not all) mesophilic prokaryotes
By using SIBR2.0-Cas12a we achieved ~70% editing efficiency, in just 48h after electroporation
November 25, 2024 at 1:47 PM
By using SIBR2.0-Cas12a we achieved ~70% editing efficiency, in just 48h after electroporation
We show that SIBR2.0 is great for inhibiting the translation of the T7 RNAP in E. coli and then we used it to tightly and inducibly express the Cas12a protein in C. necator
November 25, 2024 at 1:47 PM
We show that SIBR2.0 is great for inhibiting the translation of the T7 RNAP in E. coli and then we used it to tightly and inducibly express the Cas12a protein in C. necator
his means that even with the alternative translation site present in the SIBR sequence, a non-functional and split protein will be produced
November 25, 2024 at 1:47 PM
his means that even with the alternative translation site present in the SIBR sequence, a non-functional and split protein will be produced
This is where magic happened!! We developed SIBR2.0 :)
SIBR2.0 includes the introduction of SIBR within the coding sequence of the gene of interest, splitting it into 2 distinct exons
SIBR2.0 includes the introduction of SIBR within the coding sequence of the gene of interest, splitting it into 2 distinct exons
November 25, 2024 at 1:47 PM
This is where magic happened!! We developed SIBR2.0 :)
SIBR2.0 includes the introduction of SIBR within the coding sequence of the gene of interest, splitting it into 2 distinct exons
SIBR2.0 includes the introduction of SIBR within the coding sequence of the gene of interest, splitting it into 2 distinct exons
The alternative site for translation appeared to be gene- and organism- specific
November 25, 2024 at 1:47 PM
The alternative site for translation appeared to be gene- and organism- specific