Céline Loot
@celineloot.bsky.social
Bacterial plasticity, integrons, phage defence
DR2-CNRS, cheffe de groupe @Institut Pasteur
DR2-CNRS, cheffe de groupe @Institut Pasteur
Pinned
Céline Loot
@celineloot.bsky.social
· May 9
Sedentary chromosomal integrons as biobanks of bacterial antiphage defense systems
Integrons are genetic systems that drive bacterial adaptation by acquiring, expressing, and shuffling gene cassettes. While mobile integrons are well known for spreading antibiotic resistance genes, t...
www.science.org
At least 10% of the V. cholerae integron cassettes have novel antiphage activities (see below). Considering that there are 25,000 distinct cassettes of unknown function, integrons constitute an extraordinary and almost inexhaustible reservoir of antiphage functions!
Reposted by Céline Loot
Our new paper is out in @narjournal.bsky.social We show that natural transformation enables bacteria to shuffle integron cassettes, boosting their phenotypic diversity.
academic.oup.com/nar/article/... 1/5
academic.oup.com/nar/article/... 1/5
Bacterial natural transformation drives cassette shuffling and simplifies recombination in chromosomal integrons
Abstract. Integrons act as biobanks of gene cassettes conferring functions crucial for bacterial defense, including protection against phages and antibioti
academic.oup.com
November 17, 2025 at 3:44 PM
Our new paper is out in @narjournal.bsky.social We show that natural transformation enables bacteria to shuffle integron cassettes, boosting their phenotypic diversity.
academic.oup.com/nar/article/... 1/5
academic.oup.com/nar/article/... 1/5
Reposted by Céline Loot
Exciting news!! Our latest paper is out in Nat. Microbiol. @natmicrobiol.nature.com
We show that a sub-lineage of 7th pandemic V. cholerae has acquired mobile genetic elements packed with phage defense systems—rendering it multi-phage resistant 😳 ..... 1/3
www.nature.com/articles/s41...
We show that a sub-lineage of 7th pandemic V. cholerae has acquired mobile genetic elements packed with phage defense systems—rendering it multi-phage resistant 😳 ..... 1/3
www.nature.com/articles/s41...
West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems - Nature Microbiology
The West African–South American lineage of Vibrio cholerae contains multiple distinct anti-phage defence systems that provide resistance to various phage families, including vibriophage ICP1, a key pr...
www.nature.com
May 22, 2025 at 6:14 PM
Exciting news!! Our latest paper is out in Nat. Microbiol. @natmicrobiol.nature.com
We show that a sub-lineage of 7th pandemic V. cholerae has acquired mobile genetic elements packed with phage defense systems—rendering it multi-phage resistant 😳 ..... 1/3
www.nature.com/articles/s41...
We show that a sub-lineage of 7th pandemic V. cholerae has acquired mobile genetic elements packed with phage defense systems—rendering it multi-phage resistant 😳 ..... 1/3
www.nature.com/articles/s41...
Reposted by Céline Loot
🧫🦠🔬🧬 @science.org Sedentary chromosomal integrons as biobanks of bacterial antiphage defense systems | Science www.science.org/doi/10.1126/...
@baptistedarracq.bsky.social @eloilittner.bsky.social @celineloot.bsky.social @epcrocha.bsky.social et al.
#microbiology #phage #immunity #phagesky #MicroSky
@baptistedarracq.bsky.social @eloilittner.bsky.social @celineloot.bsky.social @epcrocha.bsky.social et al.
#microbiology #phage #immunity #phagesky #MicroSky
Sedentary chromosomal integrons as biobanks of bacterial antiphage defense systems
Integrons are genetic systems that drive bacterial adaptation by acquiring, expressing, and shuffling gene cassettes. While mobile integrons are well known for spreading antibiotic resistance genes, t...
www.science.org
May 11, 2025 at 1:25 AM
🧫🦠🔬🧬 @science.org Sedentary chromosomal integrons as biobanks of bacterial antiphage defense systems | Science www.science.org/doi/10.1126/...
@baptistedarracq.bsky.social @eloilittner.bsky.social @celineloot.bsky.social @epcrocha.bsky.social et al.
#microbiology #phage #immunity #phagesky #MicroSky
@baptistedarracq.bsky.social @eloilittner.bsky.social @celineloot.bsky.social @epcrocha.bsky.social et al.
#microbiology #phage #immunity #phagesky #MicroSky
Reposted by Céline Loot
WARNING: We have a postdoc position to work on the follow-up of this work. If you're interested, the deadline is tomorrow !!! (but I'll wait a few more days for interested candidates). research.pasteur.fr/fr/job/postd...
May 9, 2025 at 1:07 PM
WARNING: We have a postdoc position to work on the follow-up of this work. If you're interested, the deadline is tomorrow !!! (but I'll wait a few more days for interested candidates). research.pasteur.fr/fr/job/postd...
At least 10% of the V. cholerae integron cassettes have novel antiphage activities (see below). Considering that there are 25,000 distinct cassettes of unknown function, integrons constitute an extraordinary and almost inexhaustible reservoir of antiphage functions!
Sedentary chromosomal integrons as biobanks of bacterial antiphage defense systems
Integrons are genetic systems that drive bacterial adaptation by acquiring, expressing, and shuffling gene cassettes. While mobile integrons are well known for spreading antibiotic resistance genes, t...
www.science.org
May 9, 2025 at 3:26 PM
At least 10% of the V. cholerae integron cassettes have novel antiphage activities (see below). Considering that there are 25,000 distinct cassettes of unknown function, integrons constitute an extraordinary and almost inexhaustible reservoir of antiphage functions!
Reposted by Céline Loot
🧪
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Mobile integrons encode phage defense systems
Integrons are bacterial genetic elements that capture, stockpile, and modulate the expression of genes encoded in integron cassettes. Mobile integrons (MIs) are borne on plasmids, acting as a vehicle ...
www.science.org
May 8, 2025 at 8:27 PM
🧪
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Reposted by Céline Loot
White smoke, we have a new pope and also 16 new anti-phages systems in sedentary integrons (SCIs) !
In collaboration with the Rocha lab, we show in our new paper that cassettes of these large platforms encode many known anti-phage defenses, and uncovered 16 new ones.
www.science.org/doi/10.1126/...
In collaboration with the Rocha lab, we show in our new paper that cassettes of these large platforms encode many known anti-phage defenses, and uncovered 16 new ones.
www.science.org/doi/10.1126/...
Sedentary chromosomal integrons as biobanks of bacterial antiphage defense systems
Integrons are genetic systems that drive bacterial adaptation by acquiring, expressing, and shuffling gene cassettes. While mobile integrons are well known for spreading antibiotic resistance genes, t...
www.science.org
May 9, 2025 at 11:41 AM
White smoke, we have a new pope and also 16 new anti-phages systems in sedentary integrons (SCIs) !
In collaboration with the Rocha lab, we show in our new paper that cassettes of these large platforms encode many known anti-phage defenses, and uncovered 16 new ones.
www.science.org/doi/10.1126/...
In collaboration with the Rocha lab, we show in our new paper that cassettes of these large platforms encode many known anti-phage defenses, and uncovered 16 new ones.
www.science.org/doi/10.1126/...
Reposted by Céline Loot
And its out! Last work of the lab: "Evolutionary Constraints on RNA Polymerase Gene Positioning in the Genome of
Fast-Growing Bacteria."
We show how the genomic location of RNA polymerase impacts cell physiology. www.biorxiv.org/cgi/content/...
Fast-Growing Bacteria."
We show how the genomic location of RNA polymerase impacts cell physiology. www.biorxiv.org/cgi/content/...
Evolutionary Constraints on RNA Polymerase Gene Positioning in the Genome of Fast-Growing Bacteria..
The influence of gene order within the chromosome on cellular homeostasis and genome evolution remains unclear. Bacterial chromosomes are structured along the replication origin ( oriC )-terminus ( te...
www.biorxiv.org
April 15, 2025 at 4:50 AM
And its out! Last work of the lab: "Evolutionary Constraints on RNA Polymerase Gene Positioning in the Genome of
Fast-Growing Bacteria."
We show how the genomic location of RNA polymerase impacts cell physiology. www.biorxiv.org/cgi/content/...
Fast-Growing Bacteria."
We show how the genomic location of RNA polymerase impacts cell physiology. www.biorxiv.org/cgi/content/...
Reposted by Céline Loot
👋 Hi antiphage defense community, we are soon releasing an update of DefenseFinder.
We are doing our best to include all the great discoveries from the community, but with so much going on we might miss things.
Please answer wt preprints/papers with new systems or mail/git them to us. 🙏
We are doing our best to include all the great discoveries from the community, but with so much going on we might miss things.
Please answer wt preprints/papers with new systems or mail/git them to us. 🙏
January 13, 2025 at 1:54 PM
👋 Hi antiphage defense community, we are soon releasing an update of DefenseFinder.
We are doing our best to include all the great discoveries from the community, but with so much going on we might miss things.
Please answer wt preprints/papers with new systems or mail/git them to us. 🙏
We are doing our best to include all the great discoveries from the community, but with so much going on we might miss things.
Please answer wt preprints/papers with new systems or mail/git them to us. 🙏
Reposted by Céline Loot
Very happy to see Institut Pasteur on Bluesky !!!
Welcome to the official Bluesky page of the Institut Pasteur!
🔬 Explore with us the frontiers of biomedical research
🧪 Follow us to uncover groundbreaking discoveries
💡 Share knowledge for global health
🌍 Join a community passionate about progress and open science.
🔬 Explore with us the frontiers of biomedical research
🧪 Follow us to uncover groundbreaking discoveries
💡 Share knowledge for global health
🌍 Join a community passionate about progress and open science.
January 14, 2025 at 7:31 PM
Very happy to see Institut Pasteur on Bluesky !!!
Reposted by Céline Loot
Welcome to the official Bluesky page of the Institut Pasteur!
🔬 Explore with us the frontiers of biomedical research
🧪 Follow us to uncover groundbreaking discoveries
💡 Share knowledge for global health
🌍 Join a community passionate about progress and open science.
🔬 Explore with us the frontiers of biomedical research
🧪 Follow us to uncover groundbreaking discoveries
💡 Share knowledge for global health
🌍 Join a community passionate about progress and open science.
January 14, 2025 at 9:10 AM
Welcome to the official Bluesky page of the Institut Pasteur!
🔬 Explore with us the frontiers of biomedical research
🧪 Follow us to uncover groundbreaking discoveries
💡 Share knowledge for global health
🌍 Join a community passionate about progress and open science.
🔬 Explore with us the frontiers of biomedical research
🧪 Follow us to uncover groundbreaking discoveries
💡 Share knowledge for global health
🌍 Join a community passionate about progress and open science.
Reposted by Céline Loot
nature.com/articles/s41... a collaborative success of
CelineLoot (+ the rest of the PGB lab) with
@epcrocha.bsky.social
and the Biomics hub from the Pasteur
CelineLoot (+ the rest of the PGB lab) with
@epcrocha.bsky.social
and the Biomics hub from the Pasteur
January 5, 2024 at 9:52 AM
nature.com/articles/s41... a collaborative success of
CelineLoot (+ the rest of the PGB lab) with
@epcrocha.bsky.social
and the Biomics hub from the Pasteur
CelineLoot (+ the rest of the PGB lab) with
@epcrocha.bsky.social
and the Biomics hub from the Pasteur
Reposted by Céline Loot
Check out our last work with Celine Loot, Ole Skovgaard, @amazeld.bsky.social and @sanmillan.bsky.social. We have deleted the Superintegron from V. cholerae! A long-awaited feat in the field!! Despite losing 3% of the genome, the strain remains phenotipically identical across Manu conditions!! 😵
Chromosomal Integrons are Genetically and Functionally Isolated Units of Genomes
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
November 21, 2023 at 1:22 PM
Check out our last work with Celine Loot, Ole Skovgaard, @amazeld.bsky.social and @sanmillan.bsky.social. We have deleted the Superintegron from V. cholerae! A long-awaited feat in the field!! Despite losing 3% of the genome, the strain remains phenotipically identical across Manu conditions!! 😵