This enhanced MS2 quality enabled structural analysis of gastric mucins from three different species, highlighting the structural elucidation enabled by the approach.

Bonus: We also monitor the modified amino acid, giving us 2x the specificity (glycan + amino acid).

Happy to answer any questions!

We need high quality MS2 to discriminate isomers (either structural or compositional).
The higher charge states, enabled by supercharging, allow us to decipher structure and verify composition.

We recently introduced a new mobile phase for native glycan analysis. Now, we find the same reagents supercharge O-glycans, giving us up to 50% higher charge states.

Now, to exploit it...

Oxidatively released O-glycans grant additional information compared to released glycans, specifically, which amino acid is modified.

The problem? The reducing ends (glycolic or lactic acid) fix a charge on the reducing end, drastically reducing singly charged MS2 quality.

I think asking is antithetical to how these AI companies operate.

They provide a build from source option, but I disagree about containers.
It takes some time to get used to, but I'm a convert. It's a great way to get reproducible software processes.

Absolutely, and membership is still cheap. People could easily make an argument for their employer paying their dues and saving money. Back when I was a contractor I got a society membership benefit, so I paid ASMS dues myself (and had my employer pay the much-more-expensive ACS dues)

I have a feeling it might be an Agilent QTOF.

As an early tester of the column, I was very satisfied with its ability to resolve glycan structural isomers.

Product page, but worth talking to a sales rep at your next conference:
apollomics.com/product2/sho...