Big kudos to all authors of this broad collaborative approach.

And cheers to a bright future for hard X-ray nanoimaging of biological soft tissues.

@crick.ac.uk @psich.bsky.social @esrf.fr
Ana Diaz, @adrianawanner.bsky.social, @andreas-t-schaefer.bsky.social

www.nature.com/articles/s41...

This milestone unfolds the capacity of X-rays for mapping tissue ultrastructure without having to slice, which can have an impact on how we find answers in systems neuroscience and in the study of biological tissues more broadly.
tinyurl.com/XRnanocrick

We brought together a set of solutions tackling sample preparation, imaging algorithms and data processing and analysis that ultimately allowed obtaining <40nm resolution on brain tissue using X-ray ptychographic tomography. We could detect dendrites, mitochondria, myelinated axons... and synapses.

Organised by

@andreas-t-schaefer.bsky.social, @em-stp.bsky.social, @carlesbosch.bsky.social, @safekhan.bsky.social, @jkbrmn.bsky.social, @yuxinzhang.bsky.social & Anika Tailor

Register here:
tinyurl.com/crickxrays25

#CrickXrays25 is organised by @andreas-t-schaefer.bsky.social, @em-stp.bsky.social, @carlesbosch.bsky.social, @safekhan.bsky.social, @jkbrmn.bsky.social & @yuxinzhang.bsky.social with the assistance of Anika Tailor.

Hard X-ray imaging of biological soft tissues.
tinyurl.com/crickxrays25

Join us!

With invited speakers from @esrf.fr, Northwestern U, ALBA, [email protected], @psich.bsky.social, Argonne National Lab, @uni-goettingen.de, TU Denmark, @yaleschoolofmed.bsky.social, @crick.ac.uk, @ucl.ac.uk and from industry partners @dectris.bsky.social and @webknossos.org.

tinyurl.com/crickxrays25

Kudos to all authors involved and thanks to all the institutions that have hosted and supported this research, including the #synchrotrons DESY and @esrf.fr.

@crick.ac.uk @embl.org @imperialcollegeldn.bsky.social @uclnpp.bsky.social

And to the whole team: Alfred Laugros, Jayde Livingstone, Manuel Berning, Sina Tootoonian, Mihaly Kollo, Andrea Nathansen and @normanrz.com

@crick.ac.uk @uclnpp.bsky.social @esrf.fr @psich.bsky.social @zeiss-microscopy.bsky.social @webknossos.org @mpibrain.bsky.social

Enjoy the read!

Cheers to @tobiasackels.bsky.social, with whom we started the correlative workflow, to Anne Bonnin, key in the SXRT datasets that articulate the pipeline, and to Carsten Waltenberg for milling a targeted cylinder containing the neuronal circuits.

@psich.bsky.social @zeiss-microscopy.bsky.social

Big shoutout to all collaborators. Starting with @yuxinzhang.bsky.social, whose PhD evolved building this story. To @andreas-t-schaefer.bsky.social, core PI in this endeavour, and @apacureanu.bsky.social and Peter Cloetens for the expert insight on X-ray holotomography.

@crick.ac.uk @esrf.fr

We developed our correlative multimodal imaging pipeline to incorporate Synchrotron X-ray Holographic Nanotomography.
With it we mapped a targeted 0.3mm^3 volumes with subcellular detail, non-destructively.

With sufficient reliability to gather 3 replicates of the same neuronal circuit.

In a nutshell, neurons of the same olfactory circuit in the mammalian brain share firing properties while showing individual traits.

Check out the tweetorial below by @yuxinzhang.bsky.social for the main findings:

bsky.app/profile/yuxi...

That’s amazing, congrats everyone!!