Buse Isbilir
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bupbuse.bsky.social
Buse Isbilir
@bupbuse.bsky.social
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Postdoc in Bharat group at MRC-LMB | cryo-EM | cryo-ET
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Reposted by Buse Isbilir
lalouxlab.bsky.social
Apply now to attend the next EMBO Bacterial Networks meeting #EMBOBacNet

🗓️13-18 September 2026
📍Sant Feliu de Guixols, Spain

📝Program and registration info: meetings.embo.org/event/26-bac...

👩‍🔬Organised with co-chair @s-lab.bsky.social and ECR @coralietesseur.bsky.social

#MicroSky
November 20, 2025 at 10:16 AM
Reposted by Buse Isbilir
tbharat-lab.bsky.social
Assembly, architecture and functional roles of microbial surface layers

Review article published in @natrevmicro.nature.com with @bupbuse.bsky.social, Andriko von Kügelgen and @vikramalva.bsky.social.

S-layers are everywhere!

www.nature.com/articles/s41...
Assembly, architecture and functional roles of microbial surface layers - Nature Reviews Microbiology
Surface layers (S-layers) are ubiquitous protein assemblies that coat prokaryotic cells, with their functional roles increasingly coming into focus. In this Review, Isbilir and colleagues discuss rece...
www.nature.com
November 13, 2025 at 8:14 PM
Reposted by Buse Isbilir
tbharat-lab.bsky.social
An Asgard archaeon with internal membrane compartments

Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!

www.biorxiv.org/content/10.1...
November 7, 2025 at 10:44 AM
Reposted by Buse Isbilir
tbharat-lab.bsky.social
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging

Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social

www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
August 31, 2025 at 6:44 PM
Reposted by Buse Isbilir
tbharat-lab.bsky.social
Incredibly grateful to my mentors, lab members, and generous collaborators who continue to inspire our work. Thankful also to microbial cell surfaces and tomography, which continue to deliver the excitement everyday!
June 5, 2025 at 4:24 PM
Reposted by Buse Isbilir
vikramalva.bsky.social
Excited to share our new preprint on HLp—a bacterial histone from Leptospira perolatii that forms stable tetramers and wraps ~60 bp of DNA: "DNA Wrapping by a Tetrameric Bacterial Histone" www.biorxiv.org/content/10.1...

@mpi-bio-fml.bsky.social
A snapshot of the HLp tetramer (purple) wrapping ~60 bp of DNA (green).
May 12, 2025 at 9:17 AM
Reposted by Buse Isbilir
tbharat-lab.bsky.social
Finally in peer-reviewed form: Mapping the ultrastructural topology of the corynebacterial cell surface. @bupbuse.bsky.social @vikramalva.bsky.social

Please also see concurrent beautiful work from @adriasogues.bsky.social and Han Remaut.
plosbiology.org PLOS Biology @plosbiology.org · Apr 22
The unique #CellEnvelope of #corynebacteria: @tbharat-lab.bsky.social &co map the C. glutamicum cell surface, revealing a patchy S-layer & specific assembly of the PS2 protein, thereby informing our understanding of cell envelopes that contain #MycolicAcids @plosbiology.org 🧪 plos.io/3Y6JNgw
Left: The atomic model of the PS2 lattice is shown in top and side views. PS2 hexamers are repeated in the 2D sheet with a lattice constant of 176 Å. Each PS2 monomer is colored and labeled separately within the hexamer, which repeats throughout the lattice (with pore dimensions highlighted). The side view of the lattice shows the smooth surface facing the extracellular space and rough surface with coiled-coil segments protruding towards the MM. Top right: Interactions at the hexameric and trimeric interfaces are stabilized by both hydrophilic and hydrophobic residues. Bottom right: A cell envelope tomogram was used to overlay the cryo-EM structure of the S-layer onto the in situ S-layer tomographic density. A single slice of the tomogram is shown, with the overlayed PS2 S-layer lattice in green and the tomogram in grayscale. The S-layer (surface layer), MM (mycomembrane), IM (inner membrane), and Cytosol are labeled.
April 23, 2025 at 2:07 PM
Reposted by Buse Isbilir
tbharat-lab.bsky.social
Structural biology inside multicellular specimens using electron cryotomography
by @idocaspy.bsky.social @zhexinwang.bsky.social

out now in Quarterly Reviews of Biophysics
doi.org/10.1017/S003...
Structural biology inside multicellular specimens using electron cryotomography | Quarterly Reviews of Biophysics | Cambridge Core
Structural biology inside multicellular specimens using electron cryotomography - Volume 58
doi.org
February 3, 2025 at 1:54 PM