Bio-protocol
@bio-protocol.bsky.social
Bio-protocol (www.bio-protocol.org) was founded in 2011 by a group of researchers at Stanford University with a singular mission: Improving research reproducibility by publishing high-quality protocols in the life sciences.
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Bio-protocol's new issue is now live! 🎉
bio-protocol.org/en/archive?v...
bio-protocol.org/en/archive?v...
A Step-by-Step Computational Protocol for Functional Annotation and Structural Modelling of Insect Chemosensory Proteins:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
December 4, 2025 at 12:11 PM
A Step-by-Step Computational Protocol for Functional Annotation and Structural Modelling of Insect Chemosensory Proteins:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
Spheroid Sheets: A Scalable Platform for Producing Tissue Membrane Constructs
Work by Quang Bach Le, Hariharan Ezhilarasu, Weng Wan Chan and Deepak Choudhury at Agency for Science, Technology and Research.
#LifeSciences #Reproducibility #BiologicalEngineering
Work by Quang Bach Le, Hariharan Ezhilarasu, Weng Wan Chan and Deepak Choudhury at Agency for Science, Technology and Research.
#LifeSciences #Reproducibility #BiologicalEngineering
December 3, 2025 at 11:55 AM
Spheroid Sheets: A Scalable Platform for Producing Tissue Membrane Constructs
Work by Quang Bach Le, Hariharan Ezhilarasu, Weng Wan Chan and Deepak Choudhury at Agency for Science, Technology and Research.
#LifeSciences #Reproducibility #BiologicalEngineering
Work by Quang Bach Le, Hariharan Ezhilarasu, Weng Wan Chan and Deepak Choudhury at Agency for Science, Technology and Research.
#LifeSciences #Reproducibility #BiologicalEngineering
A Comparative Protocol for Preserving Deep-Water Marine Invertebrate Tissues: DNA/RNA Shield vs. Liquid Nitrogen for Dual Extraction of High-Quality Nucleic Acids:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
December 3, 2025 at 2:57 AM
A Comparative Protocol for Preserving Deep-Water Marine Invertebrate Tissues: DNA/RNA Shield vs. Liquid Nitrogen for Dual Extraction of High-Quality Nucleic Acids:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
Mouse Vestibulo-Ocular Reflex Testing for Otolith Organs and Horizontal Semicircular Canal
Work by Tong Zhao, Shijie Xiao, Qingsong Liu and Fangyi Chen at Southern University of Science and Technology and Jinxuan Liu at Giant Technologies Co.
#LifeSciences #Reproducibility #Neuroscience
Work by Tong Zhao, Shijie Xiao, Qingsong Liu and Fangyi Chen at Southern University of Science and Technology and Jinxuan Liu at Giant Technologies Co.
#LifeSciences #Reproducibility #Neuroscience
December 2, 2025 at 10:08 AM
Mouse Vestibulo-Ocular Reflex Testing for Otolith Organs and Horizontal Semicircular Canal
Work by Tong Zhao, Shijie Xiao, Qingsong Liu and Fangyi Chen at Southern University of Science and Technology and Jinxuan Liu at Giant Technologies Co.
#LifeSciences #Reproducibility #Neuroscience
Work by Tong Zhao, Shijie Xiao, Qingsong Liu and Fangyi Chen at Southern University of Science and Technology and Jinxuan Liu at Giant Technologies Co.
#LifeSciences #Reproducibility #Neuroscience
A Computational Workflow for Membrane Protein–Ligand Interaction Studies: Focus on α5-Containing GABA (A) Receptors:https://bio-protocol.org/en/bpdetail?id=5515&type=0&source=Bluesky
December 1, 2025 at 4:30 AM
A Computational Workflow for Membrane Protein–Ligand Interaction Studies: Focus on α5-Containing GABA (A) Receptors:https://bio-protocol.org/en/bpdetail?id=5515&type=0&source=Bluesky
Improved Immunohistochemistry of Mouse Eye Sections Using Davidson's Fixative and Melanin Bleaching
Work by Anne Nathalie Longakit etal. at University of British Columbia.
#LifeSciences #Reproducibility #CancerBiology
Work by Anne Nathalie Longakit etal. at University of British Columbia.
#LifeSciences #Reproducibility #CancerBiology
November 28, 2025 at 8:25 AM
Improved Immunohistochemistry of Mouse Eye Sections Using Davidson's Fixative and Melanin Bleaching
Work by Anne Nathalie Longakit etal. at University of British Columbia.
#LifeSciences #Reproducibility #CancerBiology
Work by Anne Nathalie Longakit etal. at University of British Columbia.
#LifeSciences #Reproducibility #CancerBiology
In Vivo Retroviral Transduction of Cardiac Myofibroblasts Using Intramyocardial Injection Immediately Post-myocardial Infarction
Work by Satsuki Ono etal. at Kyushu University and Michio Nakaya at Nagoya University.
#LifeSciences #Reproducibility #Medicine
Work by Satsuki Ono etal. at Kyushu University and Michio Nakaya at Nagoya University.
#LifeSciences #Reproducibility #Medicine
November 28, 2025 at 1:50 AM
In Vivo Retroviral Transduction of Cardiac Myofibroblasts Using Intramyocardial Injection Immediately Post-myocardial Infarction
Work by Satsuki Ono etal. at Kyushu University and Michio Nakaya at Nagoya University.
#LifeSciences #Reproducibility #Medicine
Work by Satsuki Ono etal. at Kyushu University and Michio Nakaya at Nagoya University.
#LifeSciences #Reproducibility #Medicine
Rapid and solvent-free, 2-hydroxyethyl methacrylate (HEMA)-acrylamide (AAm) copolymer-based optical clearing of tissue for fluorescent imaging
Work by Yanran Wang etal. at Guangdong Medical University.
#LifeSciences #Reproducibility #CellBiology
Work by Yanran Wang etal. at Guangdong Medical University.
#LifeSciences #Reproducibility #CellBiology
November 27, 2025 at 8:44 AM
Rapid and solvent-free, 2-hydroxyethyl methacrylate (HEMA)-acrylamide (AAm) copolymer-based optical clearing of tissue for fluorescent imaging
Work by Yanran Wang etal. at Guangdong Medical University.
#LifeSciences #Reproducibility #CellBiology
Work by Yanran Wang etal. at Guangdong Medical University.
#LifeSciences #Reproducibility #CellBiology
Immunopeptidomics Workflow for Isolation and LC-MS/MS Analysis of MHC Class I-Bound Peptides Under Hypoxic Conditions
Work by Hala Estephan, Ester M. Hammond and Eleni Adamopoulou at University of Oxford.
#LifeSciences #Reproducibility #CancerBiology
Work by Hala Estephan, Ester M. Hammond and Eleni Adamopoulou at University of Oxford.
#LifeSciences #Reproducibility #CancerBiology
November 27, 2025 at 2:02 AM
Immunopeptidomics Workflow for Isolation and LC-MS/MS Analysis of MHC Class I-Bound Peptides Under Hypoxic Conditions
Work by Hala Estephan, Ester M. Hammond and Eleni Adamopoulou at University of Oxford.
#LifeSciences #Reproducibility #CancerBiology
Work by Hala Estephan, Ester M. Hammond and Eleni Adamopoulou at University of Oxford.
#LifeSciences #Reproducibility #CancerBiology
Quantitative Analysis of the Arabidopsis Leaf Secretory Proteome via TMT-Based Mass Spectrometry
Work by Sakharam Waghmare etal. at University of Glasgow.
#LifeSciences #Reproducibility #PlantScience
Work by Sakharam Waghmare etal. at University of Glasgow.
#LifeSciences #Reproducibility #PlantScience
November 26, 2025 at 8:37 AM
Quantitative Analysis of the Arabidopsis Leaf Secretory Proteome via TMT-Based Mass Spectrometry
Work by Sakharam Waghmare etal. at University of Glasgow.
#LifeSciences #Reproducibility #PlantScience
Work by Sakharam Waghmare etal. at University of Glasgow.
#LifeSciences #Reproducibility #PlantScience
Characterizing Tissue Oxygen Tension During Neurogenesis in Human Cerebral Organoids
Work by Yuan-Hsuan Liu and Hsiao-Mei Wu at Research Center for Applied Sciences.
#LifeSciences #Reproducibility #Neuroscience
Work by Yuan-Hsuan Liu and Hsiao-Mei Wu at Research Center for Applied Sciences.
#LifeSciences #Reproducibility #Neuroscience
November 26, 2025 at 1:51 AM
Characterizing Tissue Oxygen Tension During Neurogenesis in Human Cerebral Organoids
Work by Yuan-Hsuan Liu and Hsiao-Mei Wu at Research Center for Applied Sciences.
#LifeSciences #Reproducibility #Neuroscience
Work by Yuan-Hsuan Liu and Hsiao-Mei Wu at Research Center for Applied Sciences.
#LifeSciences #Reproducibility #Neuroscience
Have you registered yet? 🔗https://s.bio-protocol.org/8ad9797eee9af623
Our upcoming webinar: Functional Diversity of Type I Interferons in Immune Responses
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Kathrin Sutter, Ph.D.
Moderator: Marina Kort Ph.D.
#Webinar #LifeSciences #Immunology
Our upcoming webinar: Functional Diversity of Type I Interferons in Immune Responses
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Kathrin Sutter, Ph.D.
Moderator: Marina Kort Ph.D.
#Webinar #LifeSciences #Immunology
📢 Bio-protocol Webinars present: Functional Diversity of Type I Interferons in Immune Responses
🔗 Register: s.bio-protocol.org/8ad9797eee9a...
Join us on:
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Dr. Kathrin Sutter
Moderator: Dr. Marina Kort
This #webinar is supported by BioLegend.
🔗 Register: s.bio-protocol.org/8ad9797eee9a...
Join us on:
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Dr. Kathrin Sutter
Moderator: Dr. Marina Kort
This #webinar is supported by BioLegend.
November 25, 2025 at 9:30 PM
Have you registered yet? 🔗https://s.bio-protocol.org/8ad9797eee9af623
Our upcoming webinar: Functional Diversity of Type I Interferons in Immune Responses
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Kathrin Sutter, Ph.D.
Moderator: Marina Kort Ph.D.
#Webinar #LifeSciences #Immunology
Our upcoming webinar: Functional Diversity of Type I Interferons in Immune Responses
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Kathrin Sutter, Ph.D.
Moderator: Marina Kort Ph.D.
#Webinar #LifeSciences #Immunology
Monitoring Endocytosis of Integral Membrane Proteins Using Western Blot-Based Detection of Biotinylated Antibody Uptake
Work by Alexandra Graninger and Prasanna Satpute-Krishnan at Uniformed Services University of the Health Sciences.
#LifeSciences #Reproducibility #CellBiology
Work by Alexandra Graninger and Prasanna Satpute-Krishnan at Uniformed Services University of the Health Sciences.
#LifeSciences #Reproducibility #CellBiology
November 25, 2025 at 2:53 AM
Monitoring Endocytosis of Integral Membrane Proteins Using Western Blot-Based Detection of Biotinylated Antibody Uptake
Work by Alexandra Graninger and Prasanna Satpute-Krishnan at Uniformed Services University of the Health Sciences.
#LifeSciences #Reproducibility #CellBiology
Work by Alexandra Graninger and Prasanna Satpute-Krishnan at Uniformed Services University of the Health Sciences.
#LifeSciences #Reproducibility #CellBiology
Click-qPCR: A Simple Tool for Interactive qPCR Data Analysis
Work by Azusa Kubota and Atsushi Tajima at Kanazawa University.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Work by Azusa Kubota and Atsushi Tajima at Kanazawa University.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
November 24, 2025 at 9:05 AM
Click-qPCR: A Simple Tool for Interactive qPCR Data Analysis
Work by Azusa Kubota and Atsushi Tajima at Kanazawa University.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Work by Azusa Kubota and Atsushi Tajima at Kanazawa University.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Quantifying Intestinal Glucose Absorption Using Isolated Vascularly Perfused Rat Small Intestine
Work by Cecilie Bæch-Laursen, Sabine Bæch-Laursen and Jens Juul Holst at University of Copenhagen.
#LifeSciences #Reproducibility #Biochemistry
Work by Cecilie Bæch-Laursen, Sabine Bæch-Laursen and Jens Juul Holst at University of Copenhagen.
#LifeSciences #Reproducibility #Biochemistry
November 24, 2025 at 3:38 AM
Quantifying Intestinal Glucose Absorption Using Isolated Vascularly Perfused Rat Small Intestine
Work by Cecilie Bæch-Laursen, Sabine Bæch-Laursen and Jens Juul Holst at University of Copenhagen.
#LifeSciences #Reproducibility #Biochemistry
Work by Cecilie Bæch-Laursen, Sabine Bæch-Laursen and Jens Juul Holst at University of Copenhagen.
#LifeSciences #Reproducibility #Biochemistry
Bio-protocol's new issue is now live! 🎉
bio-protocol.org/en/archive?v...
bio-protocol.org/en/archive?v...
November 21, 2025 at 2:15 AM
Bio-protocol's new issue is now live! 🎉
bio-protocol.org/en/archive?v...
bio-protocol.org/en/archive?v...
Image-Based Profiling in Live Cells Using Live Cell Painting
Work by Thaís Moraes-Lacerda etal. at Universidade Estadual de Campinas (UNICAMP) and Shantanu Singh at Broad Institute of MIT and Harvard.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Work by Thaís Moraes-Lacerda etal. at Universidade Estadual de Campinas (UNICAMP) and Shantanu Singh at Broad Institute of MIT and Harvard.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
November 20, 2025 at 5:20 AM
Image-Based Profiling in Live Cells Using Live Cell Painting
Work by Thaís Moraes-Lacerda etal. at Universidade Estadual de Campinas (UNICAMP) and Shantanu Singh at Broad Institute of MIT and Harvard.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Work by Thaís Moraes-Lacerda etal. at Universidade Estadual de Campinas (UNICAMP) and Shantanu Singh at Broad Institute of MIT and Harvard.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Fluorescence Lifetime-Based Separation of FAST-Labeled Cellular Compartment:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 19, 2025 at 8:51 AM
Fluorescence Lifetime-Based Separation of FAST-Labeled Cellular Compartment:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
A Reliable In Planta Inoculation and Antifungal Screening Protocol for Rhizoctonia solani-Induced Sheath Blight in Rice:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 19, 2025 at 2:39 AM
A Reliable In Planta Inoculation and Antifungal Screening Protocol for Rhizoctonia solani-Induced Sheath Blight in Rice:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
Generation of 3D Human iPSC-Derived Multi-Cell Type Neurospheres for Studying Neuron, Astrocyte, and Microglia Crosstalk:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 18, 2025 at 8:52 AM
Generation of 3D Human iPSC-Derived Multi-Cell Type Neurospheres for Studying Neuron, Astrocyte, and Microglia Crosstalk:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
A Practical CRISPR-Based Method for Rapid Genome Editing in Caulobacter crescentus
Work by Xuezhou Yuan, Xin Yu, Wei Zhao and Jingxian Sun at Shenzhen Institutes of Advanced Technology.
#LifeSciences #Reproducibility #Microbiology
Work by Xuezhou Yuan, Xin Yu, Wei Zhao and Jingxian Sun at Shenzhen Institutes of Advanced Technology.
#LifeSciences #Reproducibility #Microbiology
November 18, 2025 at 1:58 AM
A Practical CRISPR-Based Method for Rapid Genome Editing in Caulobacter crescentus
Work by Xuezhou Yuan, Xin Yu, Wei Zhao and Jingxian Sun at Shenzhen Institutes of Advanced Technology.
#LifeSciences #Reproducibility #Microbiology
Work by Xuezhou Yuan, Xin Yu, Wei Zhao and Jingxian Sun at Shenzhen Institutes of Advanced Technology.
#LifeSciences #Reproducibility #Microbiology
Optimized Protocol for the Collection, Cryopreservation, and In Vitro Cultivation of Human Gut Microbiota for Toxicomicrobiomics Applications:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 17, 2025 at 9:05 AM
Optimized Protocol for the Collection, Cryopreservation, and In Vitro Cultivation of Human Gut Microbiota for Toxicomicrobiomics Applications:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
📢 Bio-protocol Webinars present: Functional Diversity of Type I Interferons in Immune Responses
🔗 Register: s.bio-protocol.org/8ad9797eee9a...
Join us on:
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Dr. Kathrin Sutter
Moderator: Dr. Marina Kort
This #webinar is supported by BioLegend.
🔗 Register: s.bio-protocol.org/8ad9797eee9a...
Join us on:
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Dr. Kathrin Sutter
Moderator: Dr. Marina Kort
This #webinar is supported by BioLegend.
November 17, 2025 at 9:03 AM
📢 Bio-protocol Webinars present: Functional Diversity of Type I Interferons in Immune Responses
🔗 Register: s.bio-protocol.org/8ad9797eee9a...
Join us on:
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Dr. Kathrin Sutter
Moderator: Dr. Marina Kort
This #webinar is supported by BioLegend.
🔗 Register: s.bio-protocol.org/8ad9797eee9a...
Join us on:
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Dr. Kathrin Sutter
Moderator: Dr. Marina Kort
This #webinar is supported by BioLegend.
Protocol for the Isolation and Analysis of Extracellular Vesicles From Peripheral Blood: Red Cell, Endothelial, and Platelet-Derived Extracellular Vesicles:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 17, 2025 at 2:28 AM
Protocol for the Isolation and Analysis of Extracellular Vesicles From Peripheral Blood: Red Cell, Endothelial, and Platelet-Derived Extracellular Vesicles:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
Production of Genetically Engineered Extracellular Vesicles for Targeted Protein Delivery:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 14, 2025 at 8:08 AM
Production of Genetically Engineered Extracellular Vesicles for Targeted Protein Delivery:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...