Benoit Ballester
@benoitballester.bsky.social
Bioinformatics researcher exploring the non-coding genome. Searching for enhancer secrets. Proudly affiliated with @Inserm and @TAGC_U1090.
Preprint here: www.biorxiv.org/content/10.1...
Exonic enhancers are a widespread class of dual-function regulatory elements
Exonic enhancers (EEs) occupy an under-appreciated niche in gene regulation. By integrating transcription factor binding, chromatin accessibility, and high-throughput enhancer-reporter assays, we demo...
www.biorxiv.org
April 1, 2025 at 5:37 PM
Preprint here: www.biorxiv.org/content/10.1...
Thousands of exons carry enhancer activity. We validate EEs with STARR-seq, luciferase assays, and CRISPRi, and show disease relevance via TCGA and GWAS data.
A paradigm shift in how we interpret coding regions!
#FunctionalElements #ExonsAsEnhancers
A paradigm shift in how we interpret coding regions!
#FunctionalElements #ExonsAsEnhancers
April 1, 2025 at 5:37 PM
Thousands of exons carry enhancer activity. We validate EEs with STARR-seq, luciferase assays, and CRISPRi, and show disease relevance via TCGA and GWAS data.
A paradigm shift in how we interpret coding regions!
#FunctionalElements #ExonsAsEnhancers
A paradigm shift in how we interpret coding regions!
#FunctionalElements #ExonsAsEnhancers
Exons can regulate genes too!
Our new work shows that many coding exons are also enhancers, identified across species, and diseases. These exonic enhancers (EEs) can modulate gene expression and present regulatory variants.
#ExonEnhancers #BioRxiv #FunctionalGenomics
Our new work shows that many coding exons are also enhancers, identified across species, and diseases. These exonic enhancers (EEs) can modulate gene expression and present regulatory variants.
#ExonEnhancers #BioRxiv #FunctionalGenomics
April 1, 2025 at 5:37 PM
Exons can regulate genes too!
Our new work shows that many coding exons are also enhancers, identified across species, and diseases. These exonic enhancers (EEs) can modulate gene expression and present regulatory variants.
#ExonEnhancers #BioRxiv #FunctionalGenomics
Our new work shows that many coding exons are also enhancers, identified across species, and diseases. These exonic enhancers (EEs) can modulate gene expression and present regulatory variants.
#ExonEnhancers #BioRxiv #FunctionalGenomics
Exons ≠ just protein code. We identify 13,000+ exonic enhancers in humans and show their regulatory activity with ChIP-seq, STARR-seq, and CRISPRi.
Mutations in EEs impact expression & patient survival in cancer.
#DualFunctionDNA #CancerGenomics #cisRegulation
Mutations in EEs impact expression & patient survival in cancer.
#DualFunctionDNA #CancerGenomics #cisRegulation
April 1, 2025 at 5:37 PM
Exons ≠ just protein code. We identify 13,000+ exonic enhancers in humans and show their regulatory activity with ChIP-seq, STARR-seq, and CRISPRi.
Mutations in EEs impact expression & patient survival in cancer.
#DualFunctionDNA #CancerGenomics #cisRegulation
Mutations in EEs impact expression & patient survival in cancer.
#DualFunctionDNA #CancerGenomics #cisRegulation