Alistair Russell
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alistairrussell.bsky.social
Alistair Russell
@alistairrussell.bsky.social
190 followers 89 following 52 posts
Associate professor at UCSD studying influenza and interferons
Posts represent only my own personal opinions, for better or for worse.

russell-lab.com
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alistairrussell.bsky.social
By hooking up our promoter of interest upstream of a promoter driving a CRISPR guide, when our promoter is active a longer "signal" RNA is generated.
Description of CRITR-seq approach. A reporter promoter upstream of the U6 promoter for guide production drives the production of an mRNA that also contains the guide sequence. Sequencing is used to identify integration events, and activity of the test promoter (gDNA and mRNA, respectively). The effect of the guide at that test promoter can then be read out as the ratio of mRNA to gDNA. Edits that decrease activity at the test promoter decrease this ratio, increase, increase.
August 27, 2025 at 6:13 PM