Alison Chaves
@alisonchaves.bsky.social
Doing proteomics until I still can.
https://github.com/41ison
https://github.com/41ison
If you want to know the details. It is here: revistapesquisa.fapesp.br/en/use-of-fa...
Use of fake email addresses leads to retraction of 45 articles by Brazilian scientists
Brazilian biologist Guilherme Malafaia Pinto now ranks highly on a list that no scientist wants to be on: the researchers who have had the most scientific articles retracted (when a published paper is...
revistapesquisa.fapesp.br
November 30, 2025 at 7:43 PM
If you want to know the details. It is here: revistapesquisa.fapesp.br/en/use-of-fa...
The guy mentioned had 45 retraction, all on the same journal (Science of the Total Environment) before someone said, "Well, there is something wrong here." Academy and founding agencies reward this hyperprolific behavior. Mass spec field is not an exception.
November 30, 2025 at 2:25 PM
The guy mentioned had 45 retraction, all on the same journal (Science of the Total Environment) before someone said, "Well, there is something wrong here." Academy and founding agencies reward this hyperprolific behavior. Mass spec field is not an exception.
You can remove it, no problem. It is just attached by a magnetic part on the top. Only recommendation is to take care of the loop. When you remove the foam insulator, you will see why. The loop is a serpentine metal part that can tie a kind of knot and hold the needle causing collision.
November 29, 2025 at 10:53 PM
You can remove it, no problem. It is just attached by a magnetic part on the top. Only recommendation is to take care of the loop. When you remove the foam insulator, you will see why. The loop is a serpentine metal part that can tie a kind of knot and hold the needle causing collision.
In your picture, the needle seat is marked in the red box. The needle goes in the blue arrow. You can check this keeping the autosampler doors open, manually remove the insulator (black polystyrene part), then click the button wash in the low panel of the autosampler.
November 29, 2025 at 10:46 PM
In your picture, the needle seat is marked in the red box. The needle goes in the blue arrow. You can check this keeping the autosampler doors open, manually remove the insulator (black polystyrene part), then click the button wash in the low panel of the autosampler.
Yes, it is the sample pick up. It should not be able to see the metal. This is because the needle is constantly hitting the internal walls of the needle seat.
November 29, 2025 at 10:40 PM
Yes, it is the sample pick up. It should not be able to see the metal. This is because the needle is constantly hitting the internal walls of the needle seat.
You can't remove the block. This is a 3 parts block with an electronic plate at the bottom. If you try to remove, it may not work later. You need to use the torque tool provided with the Vanquish or the finger tight tool. That is it, patience.
November 29, 2025 at 10:32 PM
You can't remove the block. This is a 3 parts block with an electronic plate at the bottom. If you try to remove, it may not work later. You need to use the torque tool provided with the Vanquish or the finger tight tool. That is it, patience.
Once we are here, another thing that we should be ware of is the needle position at the needle seat. In this system, you need to realign the needle move periodically (usually they only tell you when the problem is there). See our picture and check your needle.
November 29, 2025 at 10:22 PM
Once we are here, another thing that we should be ware of is the needle position at the needle seat. In this system, you need to realign the needle move periodically (usually they only tell you when the problem is there). See our picture and check your needle.
You found the culprit. If you have the same error in the leak test and are not able to find the broken line, check the part marked in red in the figure with a magnifying glass:
We had one of these fractured in the loop connection.
We had one of these fractured in the loop connection.
November 29, 2025 at 10:13 PM
You found the culprit. If you have the same error in the leak test and are not able to find the broken line, check the part marked in red in the figure with a magnifying glass:
We had one of these fractured in the loop connection.
We had one of these fractured in the loop connection.
It can be a few things. Crunched fused silica lines, fracture on the tip of the peaks that were tighten, trap not well tighten. Check the peeks and the trap first. Then I would suggest to decrease the maximum pressure in the system to 1,250 bar.
November 29, 2025 at 10:05 PM
It can be a few things. Crunched fused silica lines, fracture on the tip of the peaks that were tighten, trap not well tighten. Check the peeks and the trap first. Then I would suggest to decrease the maximum pressure in the system to 1,250 bar.
Yep, the trap is inside, and you can use it in any direction.
November 29, 2025 at 8:31 PM
Yep, the trap is inside, and you can use it in any direction.
We replace this cartridge quite often. The 2 cm ones seem to be more robust, but this is cheaper.
November 29, 2025 at 8:29 PM
We replace this cartridge quite often. The 2 cm ones seem to be more robust, but this is cheaper.
Here is the link: github.com/bbuchfink/di...
GitHub - bbuchfink/diamond: Accelerated BLAST compatible local sequence aligner.
Accelerated BLAST compatible local sequence aligner. - bbuchfink/diamond
github.com
November 26, 2025 at 10:26 PM
Here is the link: github.com/bbuchfink/di...
It is quite common. Usually, physicians feel like they own the data. The statement "data will be made available under reasonable request" should be purged from any serious journal.
November 23, 2025 at 3:04 PM
It is quite common. Usually, physicians feel like they own the data. The statement "data will be made available under reasonable request" should be purged from any serious journal.
yep, a lot of time.
November 21, 2025 at 12:30 PM
yep, a lot of time.
These guys need to stop using containers. Just make things unnecessarily more complicated to run.
November 21, 2025 at 11:33 AM
These guys need to stop using containers. Just make things unnecessarily more complicated to run.
Well done, guys. I always recommend chimerys.
November 10, 2025 at 10:20 AM
Well done, guys. I always recommend chimerys.
This is the typical solution for noise. Otherwise, you can put the pumps behind a wall.
Noise reduction box for your vacuum pump share.google/INWVOMRy4bH9...
Noise reduction box for your vacuum pump share.google/INWVOMRy4bH9...
Noise reduction box for your vacuum pump
The soundproofing is optimal with a significant 75% reduction in noise perception.The interior part of our noise reduction boxes has open cells construction that delivers an incredible noise absorptio...
share.google
November 6, 2025 at 11:21 PM
This is the typical solution for noise. Otherwise, you can put the pumps behind a wall.
Noise reduction box for your vacuum pump share.google/INWVOMRy4bH9...
Noise reduction box for your vacuum pump share.google/INWVOMRy4bH9...
We are working in something that has to do with it. Statistics using report.parquet from DIA-NN using different robust estimators. limma and msqrob2 use M-estimator for robust regression. We are implementing S- and MM-estimators, as well.
October 29, 2025 at 12:52 AM
We are working in something that has to do with it. Statistics using report.parquet from DIA-NN using different robust estimators. limma and msqrob2 use M-estimator for robust regression. We are implementing S- and MM-estimators, as well.
My 5 cents: If I have more than 2 comps I always remove the intercept term as follows: model.matrix(~ 0 + groups).
I will give it a try comparing the usual limma with "ls" and "robust" vs this limpa with "ls".
I will give it a try comparing the usual limma with "ls" and "robust" vs this limpa with "ls".
October 27, 2025 at 7:40 PM
My 5 cents: If I have more than 2 comps I always remove the intercept term as follows: model.matrix(~ 0 + groups).
I will give it a try comparing the usual limma with "ls" and "robust" vs this limpa with "ls".
I will give it a try comparing the usual limma with "ls" and "robust" vs this limpa with "ls".
It sounds like Alan Sokal strikes again 😂
October 27, 2025 at 12:50 AM
It sounds like Alan Sokal strikes again 😂