The Human Biomonitoring for Ireland (HBM4IRE) project assessed the feasibility of establishing a national Human Biomonitoring (HBM) programme in Ireland to better understand cumulative chemical exposure from various sources. Despite EU regulations, concerns remain about long-term health impacts, and HBM offers a more accurate method of measuring human exposure through biological samples. The study confirmed Ireland’s capacity to implement such a programme, aligning with EU initiatives like the Green Deal and Zero Pollution Action Plan. Key outcomes include Ireland’s first priority chemical list, a proposed governance framework involving agencies like the EPA and HSE, and recommendations for stakeholder engagement, data transparency, and sustained funding. The findings support evidence-based policymaking, improved public health protections, and international collaboration. HBM4IRE also provides a roadmap for future efforts, including addressing.