Alexandre D'Halluin
@alexandredhalluin.bsky.social
Postdoctoral researcher - UMR8261 Expression Génétique Microbienne in Condon's lab.
Interested in post-transcriptionnal regulations, -omics, regulatory RNA, RNases, and sporulating bacteria 🦠
Alumni from Tine Arnvig's lab and David Hot's lab.
Interested in post-transcriptionnal regulations, -omics, regulatory RNA, RNases, and sporulating bacteria 🦠
Alumni from Tine Arnvig's lab and David Hot's lab.
🎉 Very excited to see our latest research published in @narjournal.bsky.social !! 🎉
We unvieled the mechanistic of the RNase Rae1 in B. subtilis, cleaving mRNA upstream of stalled ribosomes at their tail end !
Congrats to all the teammates for the huge work ! ✨
academic.oup.com/nar/article/...
We unvieled the mechanistic of the RNase Rae1 in B. subtilis, cleaving mRNA upstream of stalled ribosomes at their tail end !
Congrats to all the teammates for the huge work ! ✨
academic.oup.com/nar/article/...
The endoribonuclease Rae1 from Bacillus subtilis cleaves mRNA upstream of stalled ribosomes
Abstract. The ribosome-associated endoribonuclease 1 (Rae1) cleaves messenger RNAs (mRNAs) in a translation-dependent manner. Here, we identify a new Rae1
academic.oup.com
September 4, 2025 at 5:58 PM
🎉 Very excited to see our latest research published in @narjournal.bsky.social !! 🎉
We unvieled the mechanistic of the RNase Rae1 in B. subtilis, cleaving mRNA upstream of stalled ribosomes at their tail end !
Congrats to all the teammates for the huge work ! ✨
academic.oup.com/nar/article/...
We unvieled the mechanistic of the RNase Rae1 in B. subtilis, cleaving mRNA upstream of stalled ribosomes at their tail end !
Congrats to all the teammates for the huge work ! ✨
academic.oup.com/nar/article/...
Reposted by Alexandre D'Halluin
Here we show that >90% of Paenibacillus encode a unique Spo0B with a transmembrane domain! TM domain helps it interact with its phosphorelay partners. Congrats to @isabellalin.bsky.social who left for grad school this week and her mentor @cassidyprints.bsky.social! www.biorxiv.org/content/10.1...
Conservation of sporulation genes and a transmembrane-containing Spo0B variant in Paenibacillus
Sporulation is a strategy employed by many bacteria to survive harsh environmental conditions. The genus Paenibacillus includes spore-forming species notorious for spoiling pasteurized dairy products ...
www.biorxiv.org
August 28, 2025 at 11:27 PM
Here we show that >90% of Paenibacillus encode a unique Spo0B with a transmembrane domain! TM domain helps it interact with its phosphorelay partners. Congrats to @isabellalin.bsky.social who left for grad school this week and her mentor @cassidyprints.bsky.social! www.biorxiv.org/content/10.1...
Reposted by Alexandre D'Halluin
Latest from the lab! Analysis of everyone’s favorite regulatory mechanism in bacteria — the RF2 programmed frameshift! Likely present in the ancestor of bacteria, use of this mechanism is influenced by stop codon usage! Big congrats to @cassidyprints.bsky.social
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Conservation and evolution of the programmed ribosomal frameshift in prfB across the bacterial domain | mBio
Translation termination is catalyzed by one of two release factors in bacteria, RF1 or RF2. It has been known for decades that RF2 levels in Escherichia coli are regulated by a programmed ribosomal frameshift within the prfB gene that encodes RF2. We ...
journals.asm.org
August 19, 2025 at 7:44 PM
Latest from the lab! Analysis of everyone’s favorite regulatory mechanism in bacteria — the RF2 programmed frameshift! Likely present in the ancestor of bacteria, use of this mechanism is influenced by stop codon usage! Big congrats to @cassidyprints.bsky.social
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Reposted by Alexandre D'Halluin
Thrilled to share my first last-author paper, just out in #mSystems! A work about #RBPs, #RNA and #sporulation in #subtilis.
Special thanks to my brilliant student T. Kaboré who's signing hist first PhD paper and to the whole team @galinier-lab.bsky.social
👉 journals.asm.org/doi/10.1128/...
Special thanks to my brilliant student T. Kaboré who's signing hist first PhD paper and to the whole team @galinier-lab.bsky.social
👉 journals.asm.org/doi/10.1128/...
Remodeling of RNA-binding proteome and RNA-mediated regulation as a new layer of control of sporulation | mSystems
Understanding how bacteria survive extreme conditions is key to tackling challenges
in health, food safety, and industry. This study reveals a previously unexplored layer
of control in Bacillus subtil...
journals.asm.org
August 18, 2025 at 11:30 AM
Thrilled to share my first last-author paper, just out in #mSystems! A work about #RBPs, #RNA and #sporulation in #subtilis.
Special thanks to my brilliant student T. Kaboré who's signing hist first PhD paper and to the whole team @galinier-lab.bsky.social
👉 journals.asm.org/doi/10.1128/...
Special thanks to my brilliant student T. Kaboré who's signing hist first PhD paper and to the whole team @galinier-lab.bsky.social
👉 journals.asm.org/doi/10.1128/...
Reposted by Alexandre D'Halluin
We are also hiring a 4-year postdoc to study the dynamics and evolution of mobile genetic elements in the C-fixing Icelandic host-spring microbiomes
Join our exciting multidisciplinary team funded by @ukri.org BBSRC sLoLa
www.jobs.manchester.ac.uk/Job/JobDetai...
Join our exciting multidisciplinary team funded by @ukri.org BBSRC sLoLa
www.jobs.manchester.ac.uk/Job/JobDetai...
August 13, 2025 at 10:41 AM
We are also hiring a 4-year postdoc to study the dynamics and evolution of mobile genetic elements in the C-fixing Icelandic host-spring microbiomes
Join our exciting multidisciplinary team funded by @ukri.org BBSRC sLoLa
www.jobs.manchester.ac.uk/Job/JobDetai...
Join our exciting multidisciplinary team funded by @ukri.org BBSRC sLoLa
www.jobs.manchester.ac.uk/Job/JobDetai...
Reposted by Alexandre D'Halluin
EMBL is back on hiring faculty! Check out the multiple open positions:
3 PIs- 2 in Heidelberg incl. our Molecular Systems Biology Unit, 1 in Grenoble
1 Head of Unit (Cell Biology & Biophysics, Heidelberg).
Closing deadlines in Sep!
+3 more positions to come out then, stay tuned...
@embl.org
3 PIs- 2 in Heidelberg incl. our Molecular Systems Biology Unit, 1 in Grenoble
1 Head of Unit (Cell Biology & Biophysics, Heidelberg).
Closing deadlines in Sep!
+3 more positions to come out then, stay tuned...
@embl.org
August 12, 2025 at 5:25 AM
EMBL is back on hiring faculty! Check out the multiple open positions:
3 PIs- 2 in Heidelberg incl. our Molecular Systems Biology Unit, 1 in Grenoble
1 Head of Unit (Cell Biology & Biophysics, Heidelberg).
Closing deadlines in Sep!
+3 more positions to come out then, stay tuned...
@embl.org
3 PIs- 2 in Heidelberg incl. our Molecular Systems Biology Unit, 1 in Grenoble
1 Head of Unit (Cell Biology & Biophysics, Heidelberg).
Closing deadlines in Sep!
+3 more positions to come out then, stay tuned...
@embl.org
Reposted by Alexandre D'Halluin
Feels surreal to say but: I'm hiring! If you are or know anyone interested in looking at the evolution of phage resistance using A. baumannii and natural transformation, then this might be for you
www.jobbnorge.no/en/available...
www.jobbnorge.no/en/available...
Research position in Microbial Pharmacology and Population Biology (282139) | UiT The Arctic University of Norway
Job title: Research position in Microbial Pharmacology and Population Biology (282139), Employer: UiT The Arctic University of Norway, Deadline: Wednesday, August 27, 2025
www.jobbnorge.no
August 8, 2025 at 7:52 AM
Feels surreal to say but: I'm hiring! If you are or know anyone interested in looking at the evolution of phage resistance using A. baumannii and natural transformation, then this might be for you
www.jobbnorge.no/en/available...
www.jobbnorge.no/en/available...
Reposted by Alexandre D'Halluin
Our RAPPL purification of ribosomes and associated material is out. I am really proud of this study in collaboration primary with Pavlovic-Djuranovic lab, as well as Jovanovic and Hashem Labs. This method aims to replace 60 years old purification of ribosomes by sucrose cushions
rdcu.be/ezlhg
rdcu.be/ezlhg
A rapid, simple, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies
Nature Communications - Here, the authors present a method to rapidly isolate actively translating ribosomes in a time- and cost-effective manner using poly-lysine. The method is compatible with a...
rdcu.be
August 5, 2025 at 1:19 PM
Our RAPPL purification of ribosomes and associated material is out. I am really proud of this study in collaboration primary with Pavlovic-Djuranovic lab, as well as Jovanovic and Hashem Labs. This method aims to replace 60 years old purification of ribosomes by sucrose cushions
rdcu.be/ezlhg
rdcu.be/ezlhg
Sharing a new preprint thanks to @tinearnvig.bsky.social , Terry, Catherine and Declan !
We unveiled a dual mechanism of regulation of an Mtb Mbox riboswitch, combining translation regulation of a new conserved peptide and rho-dependent termination of transcription.
www.biorxiv.org/content/10.1...
We unveiled a dual mechanism of regulation of an Mtb Mbox riboswitch, combining translation regulation of a new conserved peptide and rho-dependent termination of transcription.
www.biorxiv.org/content/10.1...
A Mycobacterium tuberculosis Mbox controls a conserved, small upstream ORF via a translational expression platform and rho-dependent termination of transcription
Magnesium (Mg2+) is vital for bacterial survival, and its homeostasis is tightly regulated. Intracellular pathogens like Mycobacterium tuberculosis (Mtb) often face host-mediated magnesium limitation,...
www.biorxiv.org
August 5, 2025 at 9:44 AM
Sharing a new preprint thanks to @tinearnvig.bsky.social , Terry, Catherine and Declan !
We unveiled a dual mechanism of regulation of an Mtb Mbox riboswitch, combining translation regulation of a new conserved peptide and rho-dependent termination of transcription.
www.biorxiv.org/content/10.1...
We unveiled a dual mechanism of regulation of an Mtb Mbox riboswitch, combining translation regulation of a new conserved peptide and rho-dependent termination of transcription.
www.biorxiv.org/content/10.1...
Reposted by Alexandre D'Halluin
🍹Sharma, Jiao et al. report glycosylation of small RNAs within #exosomes and propose a glycosylation-dependent mechanism for #RNA targeting into exosomes, which may influence intercellular communication and RNA stability in the extracellular environment.
👉https://rdcu.be/eykKY
bit.ly/455vyuP
👉https://rdcu.be/eykKY
bit.ly/455vyuP
Extracellular exosomal RNAs are glyco-modified - Nature Cell Biology
Sharma, Jiao and colleagues report glycosylation of small RNAs within exosomes and propose a glycosylation-dependent mechanism for RNA targeting into exosomes, which may influence intercellular commun...
bit.ly
July 29, 2025 at 12:07 PM
🍹Sharma, Jiao et al. report glycosylation of small RNAs within #exosomes and propose a glycosylation-dependent mechanism for #RNA targeting into exosomes, which may influence intercellular communication and RNA stability in the extracellular environment.
👉https://rdcu.be/eykKY
bit.ly/455vyuP
👉https://rdcu.be/eykKY
bit.ly/455vyuP
Reposted by Alexandre D'Halluin
Frontiers to retract 122 articles, links thousands in other publishers’ journals to “unethical” network
Frontiers to retract 122 articles, links thousands in other publishers’ journals to “unethical” network
The publisher Frontiers has begun retracting a batch of 122 articles across five journals after an investigation found a network of authors and editors engaged in “unethical actions” such as manipu…
retractionwatch.com
July 29, 2025 at 10:40 AM
Frontiers to retract 122 articles, links thousands in other publishers’ journals to “unethical” network
Reposted by Alexandre D'Halluin
Happy to share the preprint from here in Shanghai, together with @danielfalush.bsky.social, as well as with team Zhizhou Jia, Huqi Wen, and Zhe Xie!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Evolution of hunt, kill, devour in a Vibrio ecospecies
Bacterial ecospecies are a recently recognized genetic structure that is hypothesized to arise by complex adaptation to a new niche. Using coadaptation analysis as a foundation for laboratory experime...
www.biorxiv.org
June 30, 2025 at 7:45 AM
Happy to share the preprint from here in Shanghai, together with @danielfalush.bsky.social, as well as with team Zhizhou Jia, Huqi Wen, and Zhe Xie!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Alexandre D'Halluin
Targeting the translation machinery: The RNase Y specificity factor Y-complex coordinates ribosome degradation https://www.biorxiv.org/content/10.1101/2025.06.27.661978v1
June 30, 2025 at 5:17 AM
Targeting the translation machinery: The RNase Y specificity factor Y-complex coordinates ribosome degradation https://www.biorxiv.org/content/10.1101/2025.06.27.661978v1
Reposted by Alexandre D'Halluin
📢 Le CNRS lance sa campagne de recrutement !
+350 postes à pourvoir dans toute la France 🌍
Inscrivez-vous aux concours et participez à l’aventure scientifique. #RejoignezleCNRS
👉 carrieres.cnrs.fr/concours-ext...
#Recherche #Emploi
+350 postes à pourvoir dans toute la France 🌍
Inscrivez-vous aux concours et participez à l’aventure scientifique. #RejoignezleCNRS
👉 carrieres.cnrs.fr/concours-ext...
#Recherche #Emploi
June 30, 2025 at 2:23 PM
📢 Le CNRS lance sa campagne de recrutement !
+350 postes à pourvoir dans toute la France 🌍
Inscrivez-vous aux concours et participez à l’aventure scientifique. #RejoignezleCNRS
👉 carrieres.cnrs.fr/concours-ext...
#Recherche #Emploi
+350 postes à pourvoir dans toute la France 🌍
Inscrivez-vous aux concours et participez à l’aventure scientifique. #RejoignezleCNRS
👉 carrieres.cnrs.fr/concours-ext...
#Recherche #Emploi
New preprint from our lab !
We have identify a new target of the endoribonuclease Rae1, flyY, and found that this RNase strictly cleaves its known targets 12nt upstream of stalled ribosomes, at their tailed end.
We have identify a new target of the endoribonuclease Rae1, flyY, and found that this RNase strictly cleaves its known targets 12nt upstream of stalled ribosomes, at their tailed end.
The endoribonuclease Rae1 from Bacillus subtilis cleaves mRNA upstream of stalled ribosomes https://www.biorxiv.org/content/10.1101/2025.06.04.657793v1
June 6, 2025 at 5:48 AM
New preprint from our lab !
We have identify a new target of the endoribonuclease Rae1, flyY, and found that this RNase strictly cleaves its known targets 12nt upstream of stalled ribosomes, at their tailed end.
We have identify a new target of the endoribonuclease Rae1, flyY, and found that this RNase strictly cleaves its known targets 12nt upstream of stalled ribosomes, at their tailed end.
Reposted by Alexandre D'Halluin
🧪
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Mobile integrons encode phage defense systems
Integrons are bacterial genetic elements that capture, stockpile, and modulate the expression of genes encoded in integron cassettes. Mobile integrons (MIs) are borne on plasmids, acting as a vehicle ...
www.science.org
May 8, 2025 at 8:27 PM
🧪
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Reposted by Alexandre D'Halluin
Quality over quantity: Small RNA pauses translation elongation to lift protein activity: Molecular Cell www.cell.com/molecular-ce...
Quality over quantity: Small RNA pauses translation elongation to lift protein activity
Small noncoding RNAs typically regulate the translation initiation of target mRNAs
and thereby protein production. In this issue of Molecular Cell, Thongdee et al. discovered
a novel regulatory mechan...
www.cell.com
May 8, 2025 at 11:01 AM
Quality over quantity: Small RNA pauses translation elongation to lift protein activity: Molecular Cell www.cell.com/molecular-ce...
Reposted by Alexandre D'Halluin
Interesting study shows CsrA forms a dynamic, membraneless compartment with sRNAs, mRNAs & the degradosome in E. coli, flipping its role between activating and repressing virulence. A new layer of RNA-based regulation! #microbiology #RNA #CsrA
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Formation of a membraneless compartment regulates bacterial virulence - Nature Communications
The RNA-binding protein CsrA switches from upregulation to downregulation of virulence genes in bacteria through unclear mechanisms. Here, the authors show that the switch between promoting and repres...
www.nature.com
April 25, 2025 at 9:10 AM
Interesting study shows CsrA forms a dynamic, membraneless compartment with sRNAs, mRNAs & the degradosome in E. coli, flipping its role between activating and repressing virulence. A new layer of RNA-based regulation! #microbiology #RNA #CsrA
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Alexandre D'Halluin
🚨 New preprint! 🚨
We just dropped our latest work on RNA-binding proteins and sporulation in B. subtilis! Turns out, the RBP landscape gets a major remix during sporulation 👀. Check it out here 👉 www.biorxiv.org/content/10.1...
#RBP #RNA #sporulation #Bacillus #microbiology @lcbofficiel.bsky.social
We just dropped our latest work on RNA-binding proteins and sporulation in B. subtilis! Turns out, the RBP landscape gets a major remix during sporulation 👀. Check it out here 👉 www.biorxiv.org/content/10.1...
#RBP #RNA #sporulation #Bacillus #microbiology @lcbofficiel.bsky.social
Remodeling of RNA-Binding Proteome and RNA-mediated regulation as a new layer of control of sporulation
Sporulation allows certain bacteria to survive extreme conditions for extended periods posing challenges to public health and food safety. Transcriptional level of regulation relying on σ factors has ...
www.biorxiv.org
April 15, 2025 at 9:27 AM
🚨 New preprint! 🚨
We just dropped our latest work on RNA-binding proteins and sporulation in B. subtilis! Turns out, the RBP landscape gets a major remix during sporulation 👀. Check it out here 👉 www.biorxiv.org/content/10.1...
#RBP #RNA #sporulation #Bacillus #microbiology @lcbofficiel.bsky.social
We just dropped our latest work on RNA-binding proteins and sporulation in B. subtilis! Turns out, the RBP landscape gets a major remix during sporulation 👀. Check it out here 👉 www.biorxiv.org/content/10.1...
#RBP #RNA #sporulation #Bacillus #microbiology @lcbofficiel.bsky.social
Reposted by Alexandre D'Halluin
Congratulations to all the candidates who qualified for the #CNRS #CRCN competition in #Section21. Such brilliant science and incredible enthusiasm! Keep it up!
c3n-cn.fr/2025/01/31/c...
c3n-cn.fr/2025/01/31/c...
Classements d’admissibilité au concours CNRS 2025
Comme en 2021, en 2022, en 2023 et en 2024 la C3N s’engage à rendre publiques les listes des candidates et des candidats admissibles classés par ordre de mérite.
c3n-cn.fr
April 11, 2025 at 7:17 PM
Congratulations to all the candidates who qualified for the #CNRS #CRCN competition in #Section21. Such brilliant science and incredible enthusiasm! Keep it up!
c3n-cn.fr/2025/01/31/c...
c3n-cn.fr/2025/01/31/c...
Reposted by Alexandre D'Halluin
If you want to do single-cell RNA-seq on your favourite bacterium, here‘s our detailed Nature Protocol for microbial MATQ-seq. It profiles up to 600 genes in a single bacterial cell!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Transcriptomic profiling of individual bacteria by MATQ-seq - Nature Protocols
Bacterial single-cell sequencing via MATQ-seq combines index sorting, random priming and rRNA depletion, and offers high cell retention and transcript capture rates, making it ideal for experiments wi...
www.nature.com
April 9, 2025 at 9:15 AM
If you want to do single-cell RNA-seq on your favourite bacterium, here‘s our detailed Nature Protocol for microbial MATQ-seq. It profiles up to 600 genes in a single bacterial cell!
www.nature.com/articles/s41...
www.nature.com/articles/s41...