Alessandro Donada
@alessandrodonada.bsky.social
"Definitely still a young hematology researcher (don’t ask how young) @Institut Curie | Big on participative research & quant bio 🧪 | Wannabe Intern at the Ministry of Silly Walks
This project was led with @perielab.bsky.social, with the help of amazing collaborators — shoutout to Gurvan, CSCO, Tiziana, and the clinicians.
Want to know more? Send me a message, or comment here!
Want to know more? Send me a message, or comment here!
a man in a helmet is looking at the camera with the words `` desire to know more intensifies '' written below him .
ALT: a man in a helmet is looking at the camera with the words `` desire to know more intensifies '' written below him .
media.tenor.com
June 25, 2025 at 9:22 AM
This project was led with @perielab.bsky.social, with the help of amazing collaborators — shoutout to Gurvan, CSCO, Tiziana, and the clinicians.
Want to know more? Send me a message, or comment here!
Want to know more? Send me a message, or comment here!
🔮 Where are we headed next?
Can we restore epigenetic structure in AML more robustly?
How stable is clonal memory over time, stress, or environment?
Let’s find out.
Can we restore epigenetic structure in AML more robustly?
How stable is clonal memory over time, stress, or environment?
Let’s find out.
a man with a beard is sitting in front of a blue wall with the words `` back to the lab again '' .
ALT: a man with a beard is sitting in front of a blue wall with the words `` back to the lab again '' .
media.tenor.com
June 25, 2025 at 9:22 AM
🔮 Where are we headed next?
Can we restore epigenetic structure in AML more robustly?
How stable is clonal memory over time, stress, or environment?
Let’s find out.
Can we restore epigenetic structure in AML more robustly?
How stable is clonal memory over time, stress, or environment?
Let’s find out.
🧨 In AML, the memory breaks down.
→ Cell families get chaotic
→ Division becomes erratic
→ Concordance is lost
But: tweaking the epigenetic landscape brings some order back.
🙏 Huge thanks to patients & clinicians who made this part possible.
→ Cell families get chaotic
→ Division becomes erratic
→ Concordance is lost
But: tweaking the epigenetic landscape brings some order back.
🙏 Huge thanks to patients & clinicians who made this part possible.
a man in a suit is carrying a stack of pizza boxes .
ALT: a man in a suit is carrying a stack of pizza boxes .
media.tenor.com
June 25, 2025 at 9:22 AM
🧨 In AML, the memory breaks down.
→ Cell families get chaotic
→ Division becomes erratic
→ Concordance is lost
But: tweaking the epigenetic landscape brings some order back.
🙏 Huge thanks to patients & clinicians who made this part possible.
→ Cell families get chaotic
→ Division becomes erratic
→ Concordance is lost
But: tweaking the epigenetic landscape brings some order back.
🙏 Huge thanks to patients & clinicians who made this part possible.
🧬 What about gene expression?
No one-size-fits-all “family module,” but expression fingerprints are shared within families. And I want to thank the @CSCO platform at Curie for the data & Tiziana Tocci for the beautiful network analysis!
No one-size-fits-all “family module,” but expression fingerprints are shared within families. And I want to thank the @CSCO platform at Curie for the data & Tiziana Tocci for the beautiful network analysis!
a cartoon character says " no no no finger prints " in front of a piano
ALT: a cartoon character says " no no no finger prints " in front of a piano
media.tenor.com
June 25, 2025 at 9:22 AM
🧬 What about gene expression?
No one-size-fits-all “family module,” but expression fingerprints are shared within families. And I want to thank the @CSCO platform at Curie for the data & Tiziana Tocci for the beautiful network analysis!
No one-size-fits-all “family module,” but expression fingerprints are shared within families. And I want to thank the @CSCO platform at Curie for the data & Tiziana Tocci for the beautiful network analysis!
📐 Could this all be random?
We built a mathematical model (thanks to Gurvan Hermange) to test it.
Still:
✅ Homogeneity in fate
✅ Concordance in division
...emerged as essential features.
We built a mathematical model (thanks to Gurvan Hermange) to test it.
Still:
✅ Homogeneity in fate
✅ Concordance in division
...emerged as essential features.
a black and white photo of a man with curly blonde hair and a mustache .
ALT: a black and white photo of a man with curly blonde hair and a mustache .
media.tenor.com
June 25, 2025 at 9:22 AM
📐 Could this all be random?
We built a mathematical model (thanks to Gurvan Hermange) to test it.
Still:
✅ Homogeneity in fate
✅ Concordance in division
...emerged as essential features.
We built a mathematical model (thanks to Gurvan Hermange) to test it.
Still:
✅ Homogeneity in fate
✅ Concordance in division
...emerged as essential features.
🧬 It’s not just division.
Cell families also:
→ Look alike
→ Choose similar fates
The big differences are between families, not within.
🧭 Lineage tracing was key.
Cell families also:
→ Look alike
→ Choose similar fates
The big differences are between families, not within.
🧭 Lineage tracing was key.
a group of bald men wearing black tank tops and sunglasses are standing next to each other
ALT: a group of bald men wearing black tank tops and sunglasses are standing next to each other
media.tenor.com
June 25, 2025 at 9:22 AM
🧬 It’s not just division.
Cell families also:
→ Look alike
→ Choose similar fates
The big differences are between families, not within.
🧭 Lineage tracing was key.
Cell families also:
→ Look alike
→ Choose similar fates
The big differences are between families, not within.
🧭 Lineage tracing was key.
👪 Cells from the same ancestor — “families” — often:
✔️ Divide the same number of times
✔️ At the same time ⏱️
And this holds across donors, media & time.
✔️ Divide the same number of times
✔️ At the same time ⏱️
And this holds across donors, media & time.
a cartoon of homer simpson driving a pink car with his family in the back seat
ALT: a cartoon of homer simpson driving a pink car with his family in the back seat
media.tenor.com
June 25, 2025 at 9:22 AM
👪 Cells from the same ancestor — “families” — often:
✔️ Divide the same number of times
✔️ At the same time ⏱️
And this holds across donors, media & time.
✔️ Divide the same number of times
✔️ At the same time ⏱️
And this holds across donors, media & time.
🔬 We took a multi-omic, single-cell approach:
Live-cell imaging
Single-cell RNA-seq (thanks CSCO platform!)
Custom assay for lineage + division + differentiation --> introducing MultiGen (DOI: 10.3791/64918)
Live-cell imaging
Single-cell RNA-seq (thanks CSCO platform!)
Custom assay for lineage + division + differentiation --> introducing MultiGen (DOI: 10.3791/64918)
patrick star from spongebob squarepants says " we have technology " in front of a computer
ALT: patrick star from spongebob squarepants says " we have technology " in front of a computer
media.tenor.com
June 25, 2025 at 9:22 AM
🔬 We took a multi-omic, single-cell approach:
Live-cell imaging
Single-cell RNA-seq (thanks CSCO platform!)
Custom assay for lineage + division + differentiation --> introducing MultiGen (DOI: 10.3791/64918)
Live-cell imaging
Single-cell RNA-seq (thanks CSCO platform!)
Custom assay for lineage + division + differentiation --> introducing MultiGen (DOI: 10.3791/64918)
Why HSPCs? 🩸
These stem cells build the entire blood system.
If they remember division patterns, it affects how we understand both healthy haematopoiesis and disease.
These stem cells build the entire blood system.
If they remember division patterns, it affects how we understand both healthy haematopoiesis and disease.
a man in a suit and tie with the words we will have to build it below him
ALT: a man in a suit and tie with the words we will have to build it below him
media.tenor.com
June 25, 2025 at 9:22 AM
Why HSPCs? 🩸
These stem cells build the entire blood system.
If they remember division patterns, it affects how we understand both healthy haematopoiesis and disease.
These stem cells build the entire blood system.
If they remember division patterns, it affects how we understand both healthy haematopoiesis and disease.
Cells divide, differentiate, and move on… right?
🧠 But we asked: do blood stem cells remember their past divisions?
Spoiler: they do — and it’s surprisingly organized.
🧠 But we asked: do blood stem cells remember their past divisions?
Spoiler: they do — and it’s surprisingly organized.
an older man playing a guitar with the words that was a long below him
ALT: an older man playing a guitar with the words that was a long below him
media.tenor.com
June 25, 2025 at 9:22 AM
Cells divide, differentiate, and move on… right?
🧠 But we asked: do blood stem cells remember their past divisions?
Spoiler: they do — and it’s surprisingly organized.
🧠 But we asked: do blood stem cells remember their past divisions?
Spoiler: they do — and it’s surprisingly organized.
Such a beautiful example of how little we know and understand about biology! In my Top3 together with tardigrades and the naked mole rat!
June 10, 2025 at 12:00 PM
Such a beautiful example of how little we know and understand about biology! In my Top3 together with tardigrades and the naked mole rat!