Here are links to two seminal papers on the application of biogenic tyrosine click to generate ADCs (pubs.acs.org/doi/10.1021/...) and BiTEs (pubs.acs.org/doi/10.1021/...).

I thank the reviewers for their constructive comments that helped us to improve the paper, and the editor for assisting us in the finalization of this paper!

Massive thanks to @chemicalscience.rsc.org, the primary Diamond OA journal for the chemical community!

SPOCQ has the great advantage over SPAAC as the proteinogenic amino acid tyrosine offers direct access to a rapid and clean method for protein modification, especially to convert therapeutic antibodies into antibody-drug conjugates (ADCs) and bispecific T cell engagers (BiTEs) (vide infra).

With deltaH++ of <2 kcal/mol, these cycloadditions are entropy-driven. This has been mentioned before in the literature, but now we have the exact numbers, obtained by stopped-flow spectroscopic analysis!

Reaction rates in SPAAC but not in SPOCQ correlated to sp-sp3 bond angles!

We used a stable quinone and a range of strained cyclic alkenes and alkynes such as BCN and DBCO for in-depth kinetic studies of the SPOCQ click reaction (which is typically >100 times faster than the more commonly used SPAAC).

For this paper, Yuri Damen grew crystals for the most-applied strained alkyne click reagents: DBCO (3077 literature references, left structure) and BCN (1680 literature references, right structure). Look how gorgeous they are!

sp-sp3 bond angles are 154.2/154.5 in BCN and 152.3/154.2 in DBCO.

Congrats Johannes!

Jahoor, er kon wel wat geregeld worden, en of mijn moeder even wilde wachten in de keuken. Ze hebben nog nooit zo lekker geluncht bij de voedselbank in Zwolle. #held #JonnieBoer #RIP

Asked = already